A further insight into the adsorption mechanism of protein on hydroxyapatite by FTIR-ATR spectrometry

Lin, Ziyu; Hu, Ren; Zhou, Jian-Zhang; Ye, Yiwen; Xu, Zhao-Xi; Lin, Cuiying

doi:10.1016/j.saa.2016.09.050

Cited by 24 publications

(11 citation statements)

References 34 publications

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“…The new peaks at 1114 cm −1 could be attributed to symmetric stretching of PO 4 3− group. The peak at 1024 cm −1 was assigned to anti‐symmetric stretching of PO 4 3− group . The peak at 948 cm −1 was due to asymmetric P‐O stretching mode, and the peak appeared at 632 cm −1 was because of symmetric P‐O stretching mode .…”

Section: Resultsmentioning

confidence: 91%

Phosphate uptake behavior and mechanism analysis of facilely synthesized nanocrystalline Zn‐Fe layered double hydroxide with chloride intercalation

Yang

Liu

Lǚ

et al. 2018

Surface & Interface Analysis

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Zn-Fe layered double hydroxide with chloride intercalation (ZFCL) was synthesized by a coprecipitation method at room temperature. ZFCL was characterized by N 2 adsorptiondesorption isotherms, X-ray diffraction, scanning electron microscope, Zeta-sizer analyzer, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The results showed that ZFCL had large surface area and layered structure. The maximum adsorption capacity of ZFCL was 150.6 mg/g at 25°C. That was higher than most other adsorbent which were reported. The kinetic data were described better by the pseudo-second-order adsorption kinetic rate model. The adsorption isotherm on the adsorbent was described by Langmuir, Freundlich, and Sips models at pH 6 and followed the fitting order: Sips >Freundlich>Langmuir. Thermodynamic analyses indicated that the phosphate adsorption on ZFCL was endothermic and spontaneous in nature. The sequence of coexisting cations and anions competing with phosphate was Ca 2+ > Mg 2+ > Na + and SO 4 2− > NO 3 − > Cl − . ZFCL can be regenerated by the sequential use of NaOH and ZnCl 2 . The adsorption capacity remained high as 108.6 mg/g after regeneration of 3 times. The results of zeta potential, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy analyses indicated that the phosphate adsorption mechanisms involved ion exchange, Zn 3 (PO 4 ) 2 precipitation, and the formation of inner-sphere complex via replacement of surface hydroxyl groups by phosphate.

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Section: Resultsmentioning

confidence: 91%

Phosphate uptake behavior and mechanism analysis of facilely synthesized nanocrystalline Zn‐Fe layered double hydroxide with chloride intercalation

Yang

Liu

Lǚ

et al. 2018

Surface & Interface Analysis

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“…The minority elements in the bone can be located as substitutional or interstitial atoms. The structural and vibrational properties of biogenic HAp [27,28] ν -COOH (G) 3,570 abc 3,571 [29] ν O-H (Absorbed H 2 O) (H) 3,360 ac 3,367 [27] ν C-H (I) 2,925 a 2,923, 2,850 [27] (J) 2,854 a Amide I (C¼O) (K) 1,642 ab 1,670 [30] 1,642 [31] Amide II (L) 1,543 1,547 [32] ν 3as CO 3 2− (M) 1,454 abc 1,430, 1,457 [31] (N) 1,412 ab 1,417 [29] 1,415-1,416, 1,460-1461 [27] ν 1s CO 3 2− (O) 1,088 abc 1,089 [33] ν 3 PO 4 3− (O) 1,088 abc 1,099 [31] (P) 1,014 abc 1,043 [27] 1,033-1,035 [28] ν 1 PO 4 3− (Q) 960 abc 960-963 [27][28][29] 978 [30] ν 2as CO 3 2− (R) 872 ab 875 [31] 871-873 [27,29] ν O-H Libration modes of HAp (S) 630 abc 632-633 [28,30] ν 4 PO 4…”

Section: Discussionmentioning

confidence: 99%

Effect of the crystal size on the infrared and Raman spectra of bio hydroxyapatite of human, bovine, and porcine bones

Londoño‐Restrepo

Zubieta‐Otero

Jeronimo‐Cruz

et al. 2019

J Raman Spectroscopy

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The optical properties of hydroxyapatites (HAps) and bio HAps have been studied using Raman and infrared (IR) spectroscopies to describe their crystalline quality. However, the size of the HAp crystals and their crystalline order effects have not been considered yet. This paper focuses on the study of the effect of the change in the crystallites size have on the width of the IR and Raman spectra for defatted and deproteinized bones as well as incinerated biogenic HAp obtained from bovine, porcine, and human bones. Bone samples were analyzed through Raman and IR spectroscopies, X‐ray diffraction (XRD), transmission electron microscopy (TEM), inductively coupled plasma (ICP), and scanning electron microscopy (SEM). The Raman and IR spectra for raw samples showed broad bands but, after calcination at 720 °C, became narrow and well defined. TEM images showed that all raw crystallites are ordered nanoplates contrary to the so far well‐established concept that biogenic HAps have low crystalline quality. XRD data confirmed that raw samples display broad peaks that correlated with the HRTEM images of ordered nanocrystals. This fact confirmed that the broad Raman and IR bands of raw clean bones come from nanocrystal plates. SEM analysis confirmed the increase in the crystal size after calcination from nanomicron to submicron dimensions due to a coalescence phenomenon. These results imply that the interpretation of Raman and IR spectra in the case of HAp nanoparticles has been erroneous. These results contribute to the design of biomaterials for tissue engineering based on biogenic HAp for bone regeneration.

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“…As subunits of the protein matrix of teeth, amino acids perform many functions, including forming a biomimetic composite with the necessary configuration to compensate for surface and volume defects in enamel and dentition. Key factors in the formation of a stable bond between the organic (protein) matrix and the phosphate complexes are not only the presence of amino acid side chains, but also the presence of uncompensated charge on the surface of the mineral complexes [ 20 , 21 , 22 ] and the conditions chosen for the synthesis processes: solvent, temperature, and pH value [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Effect of Nanocrystalline Calcium Carbonate-Substituted Hydroxyapatite and L-Lysine and L-Arginine Surface Interactions on the Molecular Properties of Dental Biomimetic Composites

et al. 2021

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Differences in the surface interactions of non-stoichiometric nanocrystalline B-type carbonate-substituted hydroxyapatite (n-cHAp) with the amino acids L-Lysine hydrochloride (L-LysHCl) and L-Arginine hydrochloride (L-ArgHCl) in acidic and alkaline media were determined using structural and spectroscopic analysis methods. The obtained data confirm that hydroxyapatite synthesized using our technique, which was used to develop the n-cHAp/L-LysHCl and n-cHAp/L-ArgHCl composites, is nanocrystalline. Studies of molecular composition of the samples by Fourier transform infrared spectroscopy under the change in the charge state of L-Lysine in environments with different alkalinity are consistent with the results of X-ray diffraction analysis, as evidenced by the redistribution of the modes’ intensities in the spectra that is correlated with the side chains, i.e., amide and carboxyl groups, of the amino acid. During the formation of a biomimetic composite containing L-Lysine hydrochloride and n-cHAp, the interaction occurred through bonding of the L-Lysine side chain and the hydroxyl groups of hydroxyapatite, which created an anionic form of L-Lysine at pH ≤ 5. In contrast, in biocomposites based on L-Arginine and n-cHAp, the interaction only slightly depends on pH value, and it proceeds by molecular orientation mechanisms. The X-ray diffraction and infrared spectroscopy results confirm that changes in the molecular composition of n-cHAp/L-ArgHCl biomimetic composites are caused by the electrostatic interaction between the L-ArgHCl molecule and the carbonate-substituted calcium hydroxyapatite. In this case, the bond formation was detected by Fourier transform infrared (FTIR) spectroscopy; the vibrational modes attributed to the main carbon chain and the guanidine group of L-Arginine are shifted during the interaction. The discovered interaction mechanisms between nanocrystalline carbonate-substituted hydroxyapatite that has physicochemical properties characteristic of the apatite in human dental enamel and specific amino acids are important for selecting the formation conditions of biomimetic composites and their integration with the natural dental tissue.

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A further insight into the adsorption mechanism of protein on hydroxyapatite by FTIR-ATR spectrometry

Cited by 24 publications

References 34 publications

Phosphate uptake behavior and mechanism analysis of facilely synthesized nanocrystalline Zn‐Fe layered double hydroxide with chloride intercalation

Phosphate uptake behavior and mechanism analysis of facilely synthesized nanocrystalline Zn‐Fe layered double hydroxide with chloride intercalation

Effect of the crystal size on the infrared and Raman spectra of bio hydroxyapatite of human, bovine, and porcine bones

Investigation of the Effect of Nanocrystalline Calcium Carbonate-Substituted Hydroxyapatite and L-Lysine and L-Arginine Surface Interactions on the Molecular Properties of Dental Biomimetic Composites

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