A further characterization of Drosophila cuticular monoenes using a mass spectrometry method to localize double bonds in complex mixtures

Pechine, J. M.; Perez, Felix; Antony, Claude; Jallon, Jean‐Marc

doi:10.1016/0003-2697(85)90344-6

Cited by 43 publications

(27 citation statements)

References 7 publications

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“…In contrast to analysis by GC/MS, it is not possible to resolve individual isomers with DART-MS; therefore, each ion signal for a hydrocarbon species corresponds to the total signal from all isomers. Despite this difference, DART profiles are consistent with many of the previous findings with GC/MS (6,9,30,31). Both methods show that the male profile is less complex than that of the female, with males expressing shorter chain unsaturated hydrocarbons and females expressing longer chain hydrocarbons with varying degrees of saturation.…”

Section: Profiling Of Male and Female Fliessupporting

confidence: 87%

Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry

Yew

Cody

Kravitz

2008

Proc. Natl. Acad. Sci. U.S.A.

114

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In mammals and insects, pheromones strongly influence social behaviors such as aggression and mate recognition. In Drosophila melanogaster, pheromones in the form of cuticular hydrocarbons play prominent roles in courtship. GC/MS is the primary analytical tool currently used to study Drosophila cuticular hydrocarbons. Although GC/MS is highly reproducible and sensitive, it requires that the fly be placed in a lethal solution of organic solvent, thereby impeding further behavioral studies. We present a technique for the analysis of hydrocarbons and other surface molecules from live animals by using direct analysis in real-time (DART) MS. Cuticular hydrocarbons were sampled from the surface of a restrained, awake behaving fly by using several brief, carefully controlled depressions of the abdomen with a small steel probe. DART mass spectral analysis of the probe detected ions with mass-to-charge ratio (m/z) of the protonated molecule corresponding to many of the previously identified unsaturated hydrocarbons. Six additional cuticular hydrocarbons also were identified. Consistent with previous GC/MS studies, male and female differences in chemical composition were evident. Spatial differences in the expression profile also were observed on males. Sampling from an individual female first as a virgin and then 45 and 90 min after successful copulation showed that mass signals likely to correspond to cis-vaccenyl acetate, tricosene, and pentacosene increased in relative intensity after courtship. This method provides near-instantaneous analysis of an individual animal's chemical profile in parallel with behavioral studies and could be extended to other models of pheromone-mediated behavior.behavior ͉ cis-vaccenyl acetate ͉ courtship ͉ pheromones ͉ Drosophila

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Section: Profiling Of Male and Female Fliessupporting

confidence: 87%

Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry

Yew

Cody

Kravitz

2008

Proc. Natl. Acad. Sci. U.S.A.

114

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“…CHs were extracted from 5-day-old individual flies by gas chromatography following hexane extraction according to the method of Pechiné et al (1985) and modified by Ferveur (1991). Analyses were performed with a Varian CP3380 chromatograph and equipped with a Cp-sil 25 mx 0.25-mm capillary column, using hydrogen as the carrier gas.…”

Section: -1mentioning

confidence: 99%

“…Peak detection was carried out using a flame ionization detector that yielded retention times and areas under each peak. All the main D. melanogaster CHs have been identified and characterized (Antony and Jallon 1982;Pechiné et al 1985Pechiné et al , 1988Jallon and Pechiné 1989). Twenty-four CHs were systematically detected in female flies and 14 in male flies, both with chain lengths ranging from 23 to 29 carbons.…”

Section: -1mentioning

confidence: 99%

A Mutation With Major Effects on Drosophila melanogaster Sex Pheromones

et al. 2005

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Sex pheromones are intraspecific chemical signals that are crucial for mate attraction and discrimination. In Drosophila melanogaster, the predominant hydrocarbons on the cuticle of mature female and male flies are radically different and tend to stimulate or inhibit male courtship, respectively. This sexual difference depends largely upon the number of double bonds (one in males and two in females) added by desaturase enzymes. A mutation was caused by a PGal4 transposon inserted in the desat1 gene that codes for the desaturase crucial for setting these double bonds. Homozygous mutant flies produced 70-90% fewer sex pheromones than control flies, and the pheromonal difference between the sexes was almost abolished. A total of 134 excision alleles were induced by pulling out all or a part of the transposon. The pheromonal profile was generally rescued in excision alleles with a completely or largely removed transposon whereas it remained mutant in alleles with a larger piece of the transposon. Five desat1 transcripts were detected during larval-to-adult development. Their levels were precisely quantified in 24-hr-old adults, a critical period for the production of sex pheromones. Three transcripts significantly varied between control females and males; however, the predominant transcript showed no difference. In mutant flies, the predominant transcript was highly decreased with the two sexually dimorphic transcripts.These two transcripts were also absent in the sibling species D. simulans, which shows no sexually dimorphic hydrocarbons. We also induced a larval-lethal allele that lacked all transcripts and failed to complement the defective hydrocarbon phenotype of mutant alleles.

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“…D. melanogaster is sexually dimorphic for hydrocarbons, with males having high levels of the 23-carbon compound cis-7-tricosene (7-T). D. melanogaster females have very little 7-T but high levels of two other hydrocarbons: the 27-carbon compound cis,cis-7,11-heptacosadiene (7,11-HD) and the 29-carbon compound cis,-cis-7,11-nonacosadiene (7,11-ND) (5,23,24). D. simulans, on the other hand, is sexually monomorphic, with both sexes having 7-T as the predominant hydrocarbon (5,14).…”

mentioning

confidence: 99%

Localization of pheromonal sexual dimorphism in Drosophila melanogaster and its effect on sexual isolation.

Coyne

Oyama

1995

Proc. Natl. Acad. Sci. U.S.A.

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Drosophila melanogaster is sexually dimorphic for cuticular hydrocarbons, with males and females having strikingly different profiles of the long-chain compounds that act as contact pheromones. Gas-chromatographic analysis of sexual mosaics reveals that the sex specificity of hydrocarbons is located in the abdomen. This explains previous observations that D. melanogaster males display the strongest courtship toward mosaics with female abdomens. We also show that males of the sibling species Drosophila simulans preferentially court D. melanogaster mosaics with male abdomens. Because the primary male hydrocarbon in D. melanogaster is also the primary female hydrocarbon in D. simulans, this supports the idea that interspecific differences in cuticular hydrocarbons contribute to sexual isolation.

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A further characterization of Drosophila cuticular monoenes using a mass spectrometry method to localize double bonds in complex mixtures

Cited by 43 publications

References 7 publications

Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry

Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry

A Mutation With Major Effects on Drosophila melanogaster Sex Pheromones

Localization of pheromonal sexual dimorphism in Drosophila melanogaster and its effect on sexual isolation.

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