A Fungal-Mediated Cryptic Selenium Cycle Linked to Manganese Biogeochemistry

Rosenfeld, Carla E.; Sabuda, Mary; Hinkle, Margaret A.G.; James, Bruce R.; Santelli, Cara M.

doi:10.1021/acs.est.9b06022

Cited by 34 publications

(38 citation statements)

References 60 publications

(122 reference statements)

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“…Abiotic controls were also prepared using the two filtered wastewater types. AY growth media abiotic controls were not established for this study, as previous work has consistently identified negligible (i.e., ≤1%) Se removal in abiotic systems without fungi ( Rosenfeld et al, 2017 , 2020 ). Ethanol-killed biomass controls were also assembled for the two filtered wastewater types.…”

Section: Methodsmentioning

confidence: 99%

“…Air-dried biomass and filters were digested along with the MCE filter in an individual perfluoroalkoxy (PFA) bottle (Thermo Fisher Scientific) by adding 4.5 mL trace metal grade nitric acid and 0.5 mL hydrogen peroxide, as described previously ( Rosenfeld et al, 2017 , 2020 ). Samples were diluted 1:1 and analyzed by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) in the Analytical Geochemistry Laboratory at the University of Minnesota.…”

Section: Methodsmentioning

confidence: 99%

“…As the carbon amendment products are proprietary, the exact composition is unknown, but the carbohydrate-based MicroC R 4000 amendment is a clear, low viscosity fluid and the glycerin-based MicroC R 2000 contains ∼71% glycerin, less than 0.1% methanol, and 0.3% fatty acid. In previous studies with these fungi, most Se removal occurred within approximately one week, depending on culture conditions (Rosenfeld et al, 2017(Rosenfeld et al, , 2020. Additionally, 7 days is a more common water treatment timeline compared to 21 days.…”

Section: Carbon-amended Experimentsmentioning

confidence: 97%

“…The Se(IV/VI) reduction rates and biomass weights measured here in AY growth media indicated that although the biomass was approximately the same weight at the end of both Se(IV and VI) experiments, more Se(IV) was removed and incorporated into the solid phase for Se(IV) experiments (Table 1; Figures 4, 6; Supplementary Tables S2-S4). Solid phase Se was likely generated through production of biomass-affiliated Se(0) nanoparticles as well as production of organoselenium compounds within the biomass (Rosenfeld et al, 2020). Future research should be directed at resolving the fungal metabolic pathways involved in both selenate and selenite reduction to provide more targeted approaches at improving the bioremediation strategy.…”

Section: Assessing and Improving The Se Mycoremediation Potentialmentioning

confidence: 99%

“…While most knowledge of microbially mediated Se transformations is related to bacterial processes, previous studies have shown that common environmental fungi are capable of reducing Se oxyanions under atmospheric conditions ( Gharieb et al, 1995 ; Rosenfeld et al, 2017 , 2018 , 2020 ; Kieliszek, 2018 ; Liang et al, 2019 ). Fungal cells have been shown to produce many more intracellular nanoparticles of Se than the average bacterium capable of Se reduction (e.g., Debieux et al, 2011 ; Butler et al, 2012 ; Rosenfeld et al, 2017 ) and are associated with higher quantities of extracellularly bound Se(0).…”

Section: Introductionmentioning

confidence: 99%

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Fungal Bioremediation of Selenium-Contaminated Industrial and Municipal Wastewaters

et al. 2020

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Selenium (Se) is an essential element for most organisms yet can cause severe negative biological consequences at elevated levels. The oxidized forms of Se, selenate [Se(VI)] and selenite [Se(IV)], are more mobile, toxic, and bioavailable than the reduced forms of Se such as volatile or solid phases. Thus, selenate and selenite pose a greater threat to ecosystems and human health. As current Se remediation technologies have varying efficiencies and costs, novel strategies to remove elevated Se levels from environments impacted by anthropogenic activities are desirable. Some common soil fungi quickly remove Se (IV and VI) from solution by aerobic reduction to solid or volatile forms. Here, we perform bench-scale culture experiments of two Se-reducing Ascomycota to determine their Se removal capacity in growth media conditions containing either Se(IV) or Se(VI) as well as in Se-containing municipal (∼25 µg/L Se) and industrial (∼2000 µg/L Se) wastewaters. Dissolved Se was measured throughout the experiments to assess Se concentration and removal rates. Additionally, solid-associated Se was quantified at the end of each experiment to determine the amount of Se removed to solid phases (e.g., Se(0) nanoparticles, biomass-adsorbed Se, or internal organic selenoproteins). Results show that under optimal conditions, fungi more efficiently remove Se(IV) from solution compared to Se(VI). Additionally, both fungi remove a higher percentage of Se from the filtered municipal wastewater compared to the industrial wastewater, though cultures in industrial wastewater retained a greater amount of solid-associated Se. Additional wastewater experiments were conducted with supplemental carbohydrate-or glycerin-based carbon products and additional nitrogenand phosphorous-containing nutrients in some cases to enhance fungal growth. Relative to unamended wastewater experiments, supplemental carbohydrates promote Se removal from municipal wastewater but minimally impact industrial wastewater removal. This demonstrates that carbon availability and source impacts fungal Se reduction and removal from solution. Calculations to assess the leaching potential of solid-associated Se from fungal biomass show that wastewater Se release will not exceed regulatory limits. This study highlights the considerable potential for the mycoremediation of Se-contaminated wastewaters.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Carbon-amended Experimentsmentioning

confidence: 97%

Section: Assessing and Improving The Se Mycoremediation Potentialmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fungal Bioremediation of Selenium-Contaminated Industrial and Municipal Wastewaters

et al. 2020

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Myco‐remediation of selenium contaminated environment and future prospects: An overview

Siddharthan,

Thangaraj,

Paulraj

et al. 2023

Environmental Quality Mgmt

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Selenium is a trace element which is an essential micronutrient for all organisms. Though it is essential, if taken in excess leads to toxicity. Hence have a detrimental effect on the health. Selenium exists in various oxidation states out of which selenite and selenate are toxic and forms the major part of the pollutant in the ecosystem. Therefore, there is an urgent need to address this issue in an eco‐friendly, efficient, and substantial way to protect the environment from further deterioration. In this regard, fungi have developed unique metabolic pathways to nullify the toxic effects of these pollutants and the process is known as mycoremediation. Mycoremediation is a cost‐effective, environmentally friendly, and efficient approach of environmental remediation that addresses organic, inorganic, and developing toxins (such as antibiotics and pharmaceuticals) by using fungus or their components. An alternative, environmentally friendly method for cleaning up contaminants in environmental matrices is provided by mycoremediation. This is due to the fact that most organic toxins are broken down rather than removed, which lowers the possibility of pollutants entering the food chain through bioaccumulation. Fungi possess robust morphology and various catabolic enzymes capable of using pollutant as a growth substrate. They transform the toxic selenium species via a series of biochemical reaction such as oxidation, reduction, methylation, and demethylation into nontoxic elemental selenium and selenium nanoparticles. Mycoremediation is a process subset that employs to fungi to degrade and restore contaminated Eco‐System. For this reason, a potential framework of “mycoremediation” was proposed to make it easier for this promising to report global environmental issues.

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Harnessing the role of selenium in soil–plant-microbe ecosystem: ecophysiological mechanisms and future prospects

et al. 2022

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A Fungal-Mediated Cryptic Selenium Cycle Linked to Manganese Biogeochemistry

Cited by 34 publications

References 60 publications

Fungal Bioremediation of Selenium-Contaminated Industrial and Municipal Wastewaters

Fungal Bioremediation of Selenium-Contaminated Industrial and Municipal Wastewaters

Myco‐remediation of selenium contaminated environment and future prospects: An overview

Harnessing the role of selenium in soil–plant-microbe ecosystem: ecophysiological mechanisms and future prospects

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