A fungal Argonaute interferes with RNA interference

Nguyen, Quyet; Iritani, Akihide; Ohkita, Shuhei; Ba, Vu Van; Yokoya, Kana; Matsubara, Ai; Ikeda, Ken; Suzuki, Nobuhiro; Nakayashiki, Hitoshi

doi:10.1093/nar/gky078

Cited by 2 publications

(3 citation statements)

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“…The new genus will also include the previously described Pyricularia oryzae ourmia‐like virus (Nguyen et al . ) which shows 46.25% identity with PsOulV1.…”

Section: Resultsmentioning

confidence: 95%

Isolation, molecular characterization and virome analysis of culturable wood fungal endophytes in esca symptomatic and asymptomatic grapevine plants

Nerva

Turina

Zanzotto

et al. 2019

Environmental Microbiology

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Summary Europe is the world largest grape producer, but in recent years, the report of diseases due to infection by grapevine trunk pathogens (GTPs) is becoming one of the main constraints for viticulture. Among grapevine trunk diseases (GTDs), esca syndrome is one of the most complex, characterized by simultaneous infection of several fungi, which leads to important reduction in yield and quality. Previous characterization of fungal isolates associated with esca syndrome leads to the recognition of at least three important players: Phaeocremonium minimum, Phaemoniella chlamydospora and Fomitiporia mediterranea. Here we isolated and characterized molecularly fungal endophytes directly from field wood tissues of plants showing or not esca symptoms. In addition, to better characterize such collection, a deep RNA sequencing (100 M reads in paired‐end) to screen for mycovirus presence was performed. Thirty‐nine viral genomes were detected, 38 of which were putative new viral species; some of these viruses infected GTPs, including P. minimum and F. mediterranea. In this work, we reported for the first time a curated collection of grapevine fungal endophytes identifying the associated mycoviruses some of which could be employed in future biotechnological exploitation as biological control agents for sustainable plant protection.

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“…The new genus will also include the previously described Pyricularia oryzae ourmia‐like virus (Nguyen et al . ) which shows 46.25% identity with PsOulV1.…”

Section: Resultsmentioning

confidence: 95%

Isolation, molecular characterization and virome analysis of culturable wood fungal endophytes in esca symptomatic and asymptomatic grapevine plants

Nerva

Turina

Zanzotto

et al. 2019

Environmental Microbiology

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“…Similar to AtAGO10, rice AGO18 ( Oryza sativa (Os)AGO18) competes with OsAGO1 for association with miR168, thereby increasing OsAGO1 protein level that is essential for antiviral RNAi in rice (Wu et al ., ). One AGO gene in fungus, Magnaporthe oryzae AGO2, interferes with RNAi likely by sequestering a broad range of siRNAs from MoAGO3 (Nguyen et al ., ). Similarly, the data herein suggest that NbAGO10 could interfere with NbAGO1‐mediated antiviral RNAi, possibly by competing with NbAGO1 for the binding of BaMV very small interfering (vsi)RNA (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…). AtAGO10, OsAGO18 and MoAGO2 have been experimentally verified for their slicer activities in vitro (Ji et al ., ; Wu et al ., ; Nguyen et al ., ), whereas NbAGO10 has the potential to cleave the target transcripts because it contains the conserved slicer catalytic sites, the DDH motif (Fig. S5).…”

Section: Discussionmentioning

confidence: 99%

Nicotiana benthamiana Argonaute10 plays a pro‐viral role in Bamboo mosaic virus infection

Huang

Tsai

et al. 2019

New Phytologist

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Summary RNA silencing is a major defense mechanism against invading viruses in plants. Argonaute proteins (AGOs) are the key players in RNA silencing. The number of AGO family members involved varies depending on the plant species and they play distinct or sometimes redundant roles in antiviral defense. By using a virus‐induced gene silencing technique, it was found that Nicotiana benthamiana AGO1 restricted Bamboo mosaic virus (BaMV) accumulation, but NbAGO10, the closest paralog of NbAGO1, positively regulated BaMV accumulation. Immunoprecipitation assay revealed BaMV virus‐derived small interfering RNAs (vsiRNAs) in NbAGO10 complexes. Transient overexpression of NbAGO10 increased BaMV RNA accumulation, but with co‐expression of NbAGO1, BaMV RNA accumulation was reduced, which suggests that NbAGO10 may have competed with NbAGO1 for sequestering BaMV vsiRNA and prevented the formation of RNA‐induced silencing complexes. In addition, overexpression of NbAGO10 decreased BaMV vsiRNA accumulation. A host enzyme, small RNA degrading nuclease 1 (SDN1), also was found to interact with NbAGO10 on in vivo pull‐down assay. Silencing of SDN1 elevated BaMV vsiRNA level and decreased BaMV RNA accumulation in N. benthamiana, indicating that NbAGO10 might recruit SDN1 for BaMV vsiRNA degradation. The results herein suggested that NbAGO10 plays a pro‐viral role by BaMV vsiRNA sequestration and degradation.

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A fungal Argonaute interferes with RNA interference

Cited by 2 publications

References 1 publication

Isolation, molecular characterization and virome analysis of culturable wood fungal endophytes in esca symptomatic and asymptomatic grapevine plants

Isolation, molecular characterization and virome analysis of culturable wood fungal endophytes in esca symptomatic and asymptomatic grapevine plants

Nicotiana benthamiana Argonaute10 plays a pro‐viral role in Bamboo mosaic virus infection

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