A functionally-distinct carboxylic acid reductase PcCAR4 unearthed from a repertoire of type IV CARs in the white-rot fungus Pycnoporus cinnabarinus

Ling, Jonathan Guyang; Mansor, Muhamad Hawari; Murad, Abdul Munir Abdul; Khalid, Rozida Mohd.; Quay, Doris Huai Xia; Winkler, Margit; Bakar, Farah Diba Abu

doi:10.1016/j.jbiotec.2019.09.008

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…The ideal pH for Mw CAR was found to be 6.5 (Supporting Information FigS3 and S4). Further CARs used in this study were Mm CAR ( Mycobacterium marinum , B2HN69.1), [21] Pc CAR4 ( Pycnoporus cinnabarinus , OM908756), [25] and Tt CAR ( Thermothelomyces thermophilus , XP_003665695.1), [26] the latter two co‐expressed with a PPTase from E. coli (ACA78686.1).…”

Section: Methodsmentioning

confidence: 99%

Aldehyde Reductase Activity of Carboxylic Acid Reductases

Winkler,

Breuer,

Schober

2024

ChemBioChem

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Carboxylic acid reductase enzymes (CARs) are well known for the reduction of a wide range of carboxylic acids to the respective aldehydes. One of the essential CAR domains ‐ the reductase domain (R‐domain) ‐ was recently shown to catalyze the standalone reduction of carbonyls, including aldehydes, which are typically considered to be the final product of carboxylic acid reduction by CAR. We discovered that the respective full‐length CARs were equally able to reduce aldehydes. Herein we aimed to shed light on the impact of this activity on aldehyde production and acid reduction in general. Our data explains previously inexplicable results and a new CAR from Mycolicibacterium wolinskyi is presented.

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Section: Methodsmentioning

confidence: 99%

Aldehyde Reductase Activity of Carboxylic Acid Reductases

Winkler,

Breuer,

Schober

2024

ChemBioChem

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Biocatalytic Carboxylate Reduction – Recent Advances and New Enzymes

Winkler

Ling

2022

ChemCatChem

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Carboxylate reductases (CARs) are valuable catalysts for the selective one‐step reduction of carboxylic acids to their corresponding aldehydes. In recent years, numerous new CARs have been made available, studied and applied in the context of biocatalytic syntheses. The preparation of aldehydes as end products for the flavor and fragrance sector and the integration of CARs in cascade reactions with aldehydes as the key intermediates represent the two major fields of applications. This review gives a comprehensive overview of the current toolbox of recombinant CARs and their numerous carboxylate substrates. Non‐natural functions of CARs are highlighted and recent insight into the structure‐function relationship of CARs are summarized.

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