A functional study of two dsRNA binding protein genes in Laodelphax striatellus

Abstract

These results indicate that the loss of LsTRBP can significantly inhibit dsChi-mediated genetic interference. These findings not only suggest that the LsTRBP gene in L. striatellus plays an important role in regulating RNAi but also imply that the metabolic regulation of dsRNAs with respect to genetic interference in L. striatellus is similar to that in higher animal species.

RNA Interference Depletion of the Halloween Gene Disembodied Implies its Potential Application for Management of Planthopper Sogatella furcifera and Laodelphax striatellus

“…In the brown planthopper Nilaparvata lugens , for example, dietary introduction of dsRNAs can not only effectively silence target genes that are mainly expressed in gut [8]–[10], or widely expressed in various tissues [11], [12], but also knock down target genes which are expressed in other tissues far from the midgut [13]. Similar results have been documented in S. furcifera and L. striatellus [14]–[16]. These results demonstrate that RNAi has great potential in planthopper management.…”

“…Moreover, it is more convenient and easy to manipulate than microinjection [8]. Oral delivery of in vitro -synthesized dsRNA has previously been used to induce gene silencing in a variety of pest insects in Lepidoptera [39]–[42], Coleoptera [43]–[47], Hymenoptera [48], Diptera [44], [49]–[53], Hemiptera [8]–[13], [16], [44], [54]–[56], Orthoptera [57] and Isoptera [58]. In the present paper, we cloned and characterized putative disembodied ( dib ) genes in two planthoppers S. furcifera and L. striatellus , and tested whether oral delivery of ds dib effectively down-regulated the target genes.…”

Biochemical and molecular characterisation and cross‐resistance in field and laboratory chlorpyrifos‐resistant strains of Laodelphax striatellus (Hemiptera: Delphacidae) from eastern China

“…Briefly, 30 fifth‐instar nymphs from the JH‐chl strain were carefully transferred into a glass tube (12 × 3 cm) with one end covered by nylon mesh and the other end sealed by stretched sealing film (Pechiney Plastic Packaging Company, Chicago, IL). dsRNAs were mixed with liquid artificial diet prepared according to Fu et al at a concentration of 0.25 µg µL −1 , and 40 µL of the mixed diet was added to the centre of the sealed end. Then, another piece of sealing film was applied to sandwich the liquid diet.…”

Non-Invasive Delivery of dsGST Is Lethal to the Sweet Potato Whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae)

“…Oral delivery of dsRNA has enormous potential applications in the management of agricultural insect pests. Diet-mediated dsRNA-induced gene silencing has been previously evidenced in a wide variety of agricultural insect pests of the order Lepidoptera [46][47][48][49], Diptera [13,[50][51][52][53], Orthoptera [54], Hymenoptera [55], Hemiptera [15,[56][57][58][59][60][61][62][63][64][65], Coleoptera [63,[66][67][68][69], and Isoptera [16]. So far, dsRNA delivery to various insect has been accomplished through oral feeding, microinjection, soaking, and transgenics [12,70,71,46].…”