A functional SNP rs895819 on pre-miR-27a is associated with bipolar disorder by targeting NCAM1

Yang, Yifeng; Lu, Wenwen; Ning, Man; Zhou, Xin; Wan, Xinyao; Mi, Qianglong; Yang, Xiao‐Jun; Zhang, Di; Zhang, Yuanyuan; Jiang, Biao; Liu, Jia; Zou, Yan

doi:10.1038/s42003-022-03263-6

Cited by 5 publications

(1 citation statement)

References 56 publications

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“…For example, the rs1321204 site on the serotonin receptor 1B (HTR1B) gene regulates the expression of HTR1B by affecting the binding of miR-96 to HTR1B, thus affecting the aggressive behavior of mice [ 28 ]. SNP rs895819, located on pre-miR-27a, is involved in the regulation of bipolar disorder by targeting neural cell adhesion molecule 1 (NCAM1) [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing

Yang,

Zhang,

Chao

et al. 2023

IJMS

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In pig production, pigs often show more aggressive behavior after mixing, which adversely affects animal welfare and growth performance. The Jumonji and structural domain-rich AT interaction domain 2 (JARID2) gene plays an important role in neurodevelopment in mice and various psychiatric disorders in humans. The JARID2 gene may impact the aggressive behavior of pigs. By observing the behavior of 500 weaned pigs during the first 72 h after mixing, the ear tissue samples of the 12 most aggressive and 12 least aggressive pigs were selected for DNA resequencing based on the intensity of their aggressive behavior. Large group correlation analysis indicated that the rs3262221458 site located in the 3′-UTR region of the porcine JARID2 gene has a strong relationship with the aggressive behavior of weaned pigs. Pigs with the mutant TT genotype of rs3262221458 have more aggressive behavior than those pigs with the GG and GT genotypes. The dual luciferase assay indicated that the luciferase activity of the plasmids containing the G allele of rs326221458 was significantly less than that of plasmids containing the T allele of rs326221458 and control groups. The binding ability of miR-9828-3p to sequences containing the T allele was less than that of sequences containing the G allele. The overexpression of miR-9828-3p in porcine neuroglial cells (PNGCs) and PK15 cells significantly decreased the mRNA and protein levels of the JARID2 gene. In addition, miR-9828-3p inhibited the proliferation of PNGCs. After inhibiting miR-9828-3p, the mRNA and protein expression levels of JARID2 increased, and the proliferation of PNGCs showed an opposite trend to the cells that forced the expression of miR-9828-3p. In addition, interference with the JARID2 gene by siRNA can effectively inhibit the proliferation of PNGCs. In summary, we found that the rs326221458 locus regulates the expression of the JARID2 gene by affecting the binding of miR-9828-3p and the JARID2 gene, thereby affecting the aggressive behavior of weaned pigs after mixing.

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Section: Discussionmentioning

confidence: 99%