A functional metagenomics study of soil carbon and nitrogen degradation networks and limiting factors on the Tibetan plateau

Yang, Chong; Zhang, Hong; Zhao, Xinquan; Liu, Pan; Wang, Lushan; Wang, Wenying

doi:10.3389/fmicb.2023.1170806

Cited by 6 publications

(1 citation statement)

References 71 publications

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“…Soil carbon and nitrogen showed the greatest impact on the structure of the soil microbial community ( Figure 6 ), suggesting that the addition of EM enhanced the influence of C, N on soil microorganisms. In degraded grasslands, the decrease in soil organic carbon and nitrogen is found to be the primary cause of reduced microbial activity ( Zeng et al, 2021 ; Yang et al, 2023 ). In this study, the addition of EM increased soil carbon and nitrogen ( Table 1 ), and the structure of soil microbial communities surely changed following the changes of carbon and nitrogen ( Guo et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Effective microorganisms input efficiently improves the vegetation and microbial community of degraded alpine grassland

Li,

Wei,

Shao

et al. 2024

Front. Microbiol.

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Soil beneficial microorganism deficiency in the degraded grasslands have emerged as the major factors negatively impacting soil quality and vegetation productivity. EM (effective microorganisms) has been regarded as a good ameliorant in improving microbial communities and restoring degraded soil of agricultural systems. However, knowledge was inadequate regarding the effects of adding EM on the degraded alpine grassland. Four levels of EM addition (0, 150, 200, 250 mL m–2) were conducted to investigate the effects of EM addition on soil properties and microorganisms of degraded alpine grassland. The addition of EM increased aboveground biomass, soil organic carbon, total nitrogen, available phosphorus, and microbial biomass, but decreased soil electric conductivity. Meanwhile, the relative biomasses of gram-negative bacteria decreased, while the ectomycorrhizal fungi and arbuscular mycorrhizal fungi increased after EM addition. The relationship between microbial communities and environmental factors has been changed. The restore effect of EM increased with the increase of addition time. These results indicated that EM addition could be a good practice to restore the health of the degraded alpine grassland ecosystem.

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Section: Discussionmentioning

confidence: 99%

Effective microorganisms input efficiently improves the vegetation and microbial community of degraded alpine grassland

Li,

Wei,

Shao

et al. 2024

Front. Microbiol.

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Metagenomic analysis reveals the effect of revegetation types on the function of soil microorganisms in carbon and nitrogen metabolism in the open-cast mining area

Zhang,

et al. 2024

Plant Soil

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AimsVegetation type is one of the most important factors contributing to the changes in soil properties during the rehabilitation process in mining areas. There is still insu cient awareness of the relationship between soil properties and the function of soil microbes in the carbon and nitrogen cycles under different revegetation type in uences. MethodsSeven reclaimed vegetation plots which has been restored for over 30 years in the Pingshuo opencast coal mine, China were established (RP: Robinia pseudoacacia, UP: Ulmus pumila, LG: Larix gmelinii, PC: Populus simonii Carr., PT: Pinus tabuliformis, PA: Picea asperata, and PS: Pinus sylvestris). The soil properties and enzyme activity were detected, while the metagenomic approach was used to analyze the potential mechanisms of soil microorganisms work in carbon and nitrogen metabolism. ResultsTotal N was obviously highest in the PC habitat (2.347 ± 0.017%), followed by RP (2.188 ± 0.059 %). The major facilitator Superfamily COG0477 and dehydrogenase the COG2133 were signi cantly enriched in the PR habitat. The acetolactate synthase COG0028 was signi cantly enriched in RP, followed by LG and PC (P < 0.05). β-glucosidase (EC3.2.1.21) were dominated in soil microbial communities among 7 vegetation types, and signi cantly richer in RP and LG, followed by PC and PT (P < 0.05). GTs and GHswere signi cantly richer in UP and LG, respectively. ConclusionRobinia pseudoacacia and Larix gmelinii may be good species choice for improving the productivity and stability of the soil ecosystem in mining areas. Highlights• The revegetation species affect soil nutrient conditions and enzyme activities.• COG0477, COG2133 and COG0028 are abundant in R. pseudoacacia habits.• β-glucosidase genes dominate among all of the revegetation habits.• R. pseudoacacia and L. gmelinii are excellent species for reclamation in mining area.

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Combined metagenomics and metabolomic analysis of microbial community structure and metabolic function in continuous soybean cropping soils of Songnen Plain, China

Xu,

Jin,

et al. 2024

Chem. Biol. Technol. Agric.

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Continuous cropping has a negative effect on soybean yield. In this study, a positioning experiment was conducted starting in 2015, with three treatments: maize–soybean rotation (SMR), 2-year maize, 2-year soybean rotation cropping (SC2), and 8-year soybean continuous cropping (SC8). We determined soybean yields (2015–2022) and analyzed soil microbial communities, functions, and metabolites composition in the 0–20 cm tillage layer using metagenomics technology and GC–MS technology during soybean flowering in 2022. Results indicated that continuous cropping (SC8) significantly reduced soybean yield compared to crop rotation (SMR) during the experimental period, while SC8 showed higher yield than SC2 in 2022. Compared to SMR, SC8 significantly increased soil N content and significantly decreased pH and TP, AP, and AK content. However, the pH and AK contents of SC8 were significantly higher than those of SC2. LeFSe analysis showed that Friedmanniella, Microlunatus, Nitrososphaera, Rubrobacter, Geodermatophilus, Nitriliruptor were enriched in SC8. Gaiella, Sphaerobacter, Methyloceanibacter were enriched in SC2. Sphingomonas, Cryobacterium, Marmoricola, Haliangium, Arthrobacter, Ramlibacter, Rhizobacter, Pseudolabrys, Methylibium, Variovorax were enriched in SMR. And the relative abundance of Cryobacterium, Marmoricola, Haliangium, Arthrobacter, Ramlibacter, Rhizobacter, Methylibium, Variovorax was significantly positively correlated with yield, while the relative abundance of Gaiella and Sphaerobacter was significantly negatively correlated with yield. SC8 significantly increased the abundance of genes in nitrogen metabolism and significantly decreased the abundance of genes related to phosphorus and potassium metabolism compared with SMR. However, the abundance of genes in potassium metabolism was significantly higher in SC8 than in SC2. Metabolomic analysis showed that compared to SMR, SC8 decreased the abundance of carbohydrates, ketones, and lipid. However, the abundance of carbohydrates, ketones, and lipid was significantly higher in SC8 than in SC2. Mantel test showed that soil pH and AK significantly affected soil microbial community, function, and metabolite composition. Correlation analysis showed significant correlation between soil metabolites and microorganisms, metabolic functions. Graphical Abstract

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A functional metagenomics study of soil carbon and nitrogen degradation networks and limiting factors on the Tibetan plateau

Cited by 6 publications

References 71 publications

Effective microorganisms input efficiently improves the vegetation and microbial community of degraded alpine grassland

Effective microorganisms input efficiently improves the vegetation and microbial community of degraded alpine grassland

Metagenomic analysis reveals the effect of revegetation types on the function of soil microorganisms in carbon and nitrogen metabolism in the open-cast mining area

Combined metagenomics and metabolomic analysis of microbial community structure and metabolic function in continuous soybean cropping soils of Songnen Plain, China

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