A functional in vitro model of rat blood–brain barrier for molecular analysis of efflux transporters

Perrière, Nicolas; Yousif, Salah; Cazaubon, Sylvie; Chaverot, Nathalie; Bourasset, Fanchon; Cisternino, Salvatore; Declèves, Xavier; Hori, Satoko; Terasaki, Tetsuya; Deli, Mária A.; Scherrmann, Jean Michel; Temsamani, Jamal; Roux, F.; Couraud, Pierre Olivier

doi:10.1016/j.brainres.2007.02.091

Cited by 137 publications

(126 citation statements)

References 47 publications

Supporting

Mentioning

113

Contrasting

Order By: Relevance

“…This finding is in agreement with data on the favourable effect of hydrocortisone on epithelial cells described previously (Wu et al 1986;Van Scott et al 1988). Hydrocortisone is a potent inducer of the formation of barrier properties in cultured endothelial cells (Perrière et al 2007;Nakagawa et al 2009), and the increase in the cell index of RPMI 2650 layers may be also related to the formation of a tighter barrier. Cholera toxin, which acts through cAMP as a second messenger, improves cell growth and inhibits the differentiation-inducing activity of serum (Lechner 1984;Wu et al 1986).…”

Section: Resultssupporting

confidence: 93%

Retinoic acid and hydrocortisone strengthen the barrier function of human RPMI 2650 cells, a model for nasal epithelial permeability

et al. 2012

View full text Add to dashboard Cite

The nasal pathway represents an alternative route for non-invasive systemic administration of drugs. The main advantages of nasal drug delivery are the rapid onset of action, the avoidance of the first-pass metabolism in the liver and the easy applicability. In vitro cell culture systems offer an opportunity to model biological barriers. Our aim was to develop and characterize an in vitro model based on confluent layers of the human RPMI 2650 cell line. Retinoic acid, hydrocortisone and cyclic adenosine monophosphate, which influence cell attachment, growth and differentiation have been investigated on the barrier formation and function of the nasal epithelial cell layers. Real-time cell microelectronic sensing, a novel label-free technique was used for dynamic monitoring of cell growth and barrier properties of RPMI 2650 cells. Treatments enhanced the formation of adherens and tight intercellular junctions visualized by electron microscopy, the presence and localization of junctional proteins ZO-1 and b-catenin demonstrated by fluorescent immunohistochemistry, and the barrier function of nasal epithelial cell layers. The transepithelial resistance of the RPMI 2650 cell model reached 50 to 200 X 9 cm 2 , the permeability coefficient for 4.4 kDa FITC-dextran was 9.3 to 17 9 10 -6 cm/s, in agreement with values measured on nasal mucosa from in vivo and ex vivo experiments. Based on these results human RPMI 2650 cells seem to be a suitable nasal epithelial model to test different pharmaceutical excipients and various novel formulations, such as nanoparticles for toxicity and permeability.

show abstract

Section: Resultssupporting

confidence: 93%

Retinoic acid and hydrocortisone strengthen the barrier function of human RPMI 2650 cells, a model for nasal epithelial permeability

et al. 2012

View full text Add to dashboard Cite

show abstract

“…In comparison, the benzodiazepine drug diazepam that is used as anxiolytic and has large BBB uptake 45 shows 10-fold higher permeability than LY using the hCMEC/D3 cell line (data not shown). This means that compound 16 shows an intermediate in vitro permeability to the brain.…”

Section: Resultsmentioning

confidence: 98%

Synthesis and Antiprotozoal Activity of N-Alkoxy Analogues of the Trypanocidal Lead Compound 4,4′-Bis(imidazolinylamino)diphenylamine with Improved Human Blood−Brain Barrier Permeability

et al. 2010

View full text Add to dashboard Cite

“…cultures of both primary and immortalised cells of rat, mouse and human origin (Beaulieu et al, 1997;Begley et al, 1996;Miller et al, 2000;Perriere et al, 2007;Seetharaman et al, 1998;Tatsuta et al, 1992;Virgintino et al, 2002). In terms of Pgp expression, the BEC phenotype appears to be preserved in hCMEC/D3 cells over a wide range of passages, and P-gp expression was comparable to cultured primary BECs, albeit slightly reduced.…”

Section: Discussionmentioning

confidence: 99%

Polarized P-glycoprotein expression by the immortalised human brain endothelial cell line, hCMEC/D3, restricts apical-to-basolateral permeability to rhodamine 123

et al. 2009

View full text Add to dashboard Cite

Polarized P-glycoprotein expression by the immortalised human brain endothelial cell line, hCMEC/D3, restricts apical-to-basolateral permeability to rhodamine 123 Leon M. Tai This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. PMSF, phenylmethylsulphonyl fluoride; rh123, rhodamine 123; EM, electron microscopy. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT-1 - A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

show abstract

A functional in vitro model of rat blood–brain barrier for molecular analysis of efflux transporters

Cited by 137 publications

References 47 publications

Retinoic acid and hydrocortisone strengthen the barrier function of human RPMI 2650 cells, a model for nasal epithelial permeability

Retinoic acid and hydrocortisone strengthen the barrier function of human RPMI 2650 cells, a model for nasal epithelial permeability

Synthesis and Antiprotozoal Activity of N-Alkoxy Analogues of the Trypanocidal Lead Compound 4,4′-Bis(imidazolinylamino)diphenylamine with Improved Human Blood−Brain Barrier Permeability

Polarized P-glycoprotein expression by the immortalised human brain endothelial cell line, hCMEC/D3, restricts apical-to-basolateral permeability to rhodamine 123

Contact Info

Product

Resources

About