A Functional Genomics Strategy to Identify Genes That Regulate the Production of Biologically Active Metabolites in Plants

Falcone, Deane L.; Rogers, Dennis T.; Yun, Kil-Young; Diniello, Gabriela; Fu, May C.; Artiushin, Irina A.; Littleton, John M.

doi:10.1007/978-94-017-2679-5_98

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…Several strategies have been proposed recently to address the challenges and complexity [2, 11, 13]. …”

Section: Rational Approach: Strategies and Technical Frameworkmentioning

confidence: 99%

“…The regulation and manipulation of complimentary pathways provides the means to sustain the targeted pathways, while the blocking of competing pathways is critical for redirecting metabolic flux. One example of these strategies was illustrated by the combination of activation tagging mutagenesis with high-throughput screening for biological active metabolites, which enabled the isolation of genetic material relevant to the synthesis of specific natural products [13]. This approach involved the generation of a callus library via plant cell mutagenesis prepared by activation T-DNA tagging [15], the development of a high-throughput screen assay for the targeted metabolites, and the functional genomics analysis of the overexpressing cell lines.…”

Section: Rational Approach: Strategies and Technical Frameworkmentioning

confidence: 99%

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To Stretch the Boundary of Secondary Metabolite Production in Plant Cell‐Based Bioprocessing: Anthocyanin as a Case Study

Zhang

Franco

Curtin

et al. 2004

BioMed Research International

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Plant cells and tissue cultures hold great promise for controlled production of a myriad of useful secondary metabolites on demand. The current yield and productivity cannot fulfill the commercial goal of a plant cell-based bioprocess for the production of most secondary metabolites. In order to stretch the boundary, recent advances, new directions and opportunities in plant cell-based bioprocessing have been critically examined for the 10 years from 1992 to 2002. A review of the literature indicated that most of the R&D work was devoted predominantly to studies at an empirical level. A rational approach to molecular plant cell bioprocessing based on the fundamental understanding of metabolic pathways and their regulations is urgently required to stimulate further advances; however, the strategies and technical framework are still being developed. It is the aim of this review to take a step forward in framing workable strategies and technologies for molecular plant cell-based bioprocessing. Using anthocyanin biosynthesis as a case study, an integrated postgenomic approach has been proposed. This combines the functional analysis of metabolic pathways for biosynthesis of a particular metabolite from profiling of gene expression and protein expression to metabolic profiling. A global correlation not only can thus be established at the three molecular levels, but also places emphasis on the interactions between primary metabolism and secondary metabolism; between competing and/or complimentary pathways; and between biosynthetic and post-biosynthetic events.

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“…Several strategies have been proposed recently to address the challenges and complexity [2, 11, 13]. …”

Section: Rational Approach: Strategies and Technical Frameworkmentioning

confidence: 99%

Section: Rational Approach: Strategies and Technical Frameworkmentioning

confidence: 99%

To Stretch the Boundary of Secondary Metabolite Production in Plant Cell‐Based Bioprocessing: Anthocyanin as a Case Study

Zhang

Franco

Curtin

et al. 2004

BioMed Research International

View full text Add to dashboard Cite

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Natural Products Genomics: A novel approach for the discovery of anti-cancer therapeutics

Gunjan

Rogers

et al. 2011

Journal of Pharmacological and Toxicological Methods

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The future of plant drug discovery

Littleton

2007

Expert Opinion on Drug Discovery

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To have a future in the pharmaceutical industry, plant drug discovery must compete with combinatorial chemistry and high-throughput pharmacologic screening (HTPS). Plant functional genomics coupled with HTPS may achieve this; thus, functional biology can identify 'libraries' of candidate plant species from which individuals can be prioritized by 'differential HTPS'. The full genomic potential of a species for bioactivity can be accessed by cellular mutagenesis and elicitation, with HTPS identifying clones with novel activity. The comparison of 'positive' clonal phenotypes with their almost identical 'negatives' facilitates the identification of active compounds and 'genomic' products. The logical conclusion is to use combinatorial genomics together with HTPS to direct plant cellular 'evolution' continuously towards metabolites with specific pharmacologic activity. Mankind would then become the orchestrator of plant secondary metabolism, rather than its passive beneficiary.

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A Functional Genomics Strategy to Identify Genes That Regulate the Production of Biologically Active Metabolites in Plants

Cited by 3 publications

References 4 publications

To Stretch the Boundary of Secondary Metabolite Production in Plant Cell‐Based Bioprocessing: Anthocyanin as a Case Study

To Stretch the Boundary of Secondary Metabolite Production in Plant Cell‐Based Bioprocessing: Anthocyanin as a Case Study

Natural Products Genomics: A novel approach for the discovery of anti-cancer therapeutics

The future of plant drug discovery

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