A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus

Bol, John F.; Vloten-Doting, Lous Van; Jaspars, E.M.J.

doi:10.1016/0042-6822(71)90007-9

Cited by 207 publications

(70 citation statements)

References 21 publications

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“…2 (a) shows an electrophoretic separation of the virus particles produced in protoplasts inoculated with B + M + Tb. The virus components were produced in a ratio that differed from the component composition obtained with intact tobacco plants (Bol et al, 1971). The nucleoprotein patterns obtained after inoculation with B + M (Fig.…”

Section: Synthesis Of Viral Coat Protein and Nucleoproteinmentioning

confidence: 99%

“…To initiate infection of tobacco or bean plants, a mixture of the three genome segments, RNAs 1, 2 and 3, has to be supplemented with a small amount of coat protein, or its subgenomic messenger, RNA 4, which is present in a fourth class of particle, top component a (Ta) (Bol et al, 1971). It has been suggested that a specific binding of coat protein near the 3'-termini of the viral RNAs is required for a proper recognition of the genome segments by the viral replicase (Houwing & Jaspars, 1978;Koper-Zwarthoff & Bol, 1980).…”

Section: Introductionmentioning

confidence: 99%

“…20000), collected by centrifugation and resuspended in 0.1 ml of a 10 mM-phosphate buffer pH 7. This suspension was loaded on to a cylindrical 3 % polyacrylamide gel and viral nucleoproteins were separated by electrophoresis as described previously (Bol et al, 1971).…”

Section: Introductionmentioning

confidence: 99%

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Localization of Genetic Information Involved in the Replication of Alfalfa Mosaic Virus

Nassuth

Alblas

Bol

1981

Journal of General Virology

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SUMMARYCowpea protoplasts were inoculated with different combinations of the four nucleoprotein components of alfalfa mosaic virus (A1MV), and the synthesis of viral particles, coat protein and RNA was analysed. Synthesis of viral particles and coat protein was detectable by polyacrylamide gel electrophoresis only when the inoculum contained the components B, M and Tb, accommodating RNAs 1, 2 and 3 respectively.Upon inoculation with B + M + Tb, the synthesis of viral RNA was detectable by annealing of radiolabelled RNA from protoplasts to virus-specific double-stranded RNA. On inoculation with B + Tb or M ÷ Tb no significant amount of viral RNA was produced. After inoculation with B + M, however, the production of virus-specifiC RNA was about 40 % of that induced by complete infection, indicating that genetic information encoded by RNAs 1 and 2 is involved in viral RNA replication. As a mixture of the genomic RNAs did not induce the synthesis of virus-specific RNA, it is concluded that coat protein is required for the RNA synthesis induced by the mixture of B and M nucleoproteins.The observation that no coat protein is produced upon inoculation with B + M + Ta components indicates that the replicase activity induced by B + M does not replicate RNA 4, the messenger for coat protein present in Ta particles.

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Section: Synthesis Of Viral Coat Protein and Nucleoproteinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Localization of Genetic Information Involved in the Replication of Alfalfa Mosaic Virus

Nassuth

Alblas

Bol

1981

Journal of General Virology

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“…CP is translated from a subgenomic messenger, RNA4. A mixture of the three genomic RNAs is not infectious unless a few CP molecules are added per RNA molecule (Bol et al, 1971). This phenomenon is called genome activation.…”

Section: Introductionmentioning

confidence: 99%

Alfalfa mosaic virus RNA3 mutants do not replicate in transgenic plants expressing RNA3-specific genes

Dore

Dun

Pinck

et al. 1991

Journal of General Virology

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The RNA3 of alfalfa mosaic virus (A1MV) encodes the P3 protein and the viral coat protein (CP). RNA3 molecules transcribed in vitro replicated in protoplasts and plants when inoculated in mixtures with AIMV RNA 1, RNA2 and CP. Transcripts with a deletion or inversion in the P3 gene replicated well in protoplasts but not in transgenic plants transformed with the P3 gene. Transgenic plants expressing the CP gene became infected after inoculation with a mixture of RNA 1, RNA2 and wild-type RNA3 transcripts without addition of CP to the inoculum. Transcripts with a deletion in the CP gene replicated at a reduced level in protoplasts but not in CP-transformed plants. This suggests that P3 and CP are both required for cell-tocell spread of AIMV and that mutations in the inoculum RNA could not be complemented in trans by the wild-type chimeric nuclear genes.

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“…We chose this system to produce c(g amounts of the gene products of the tripartite genome of alfalfa mosaic virus (AMV) [4]. One of the problems encountered when scaling up the in vitro protein synthesis was the large amount of relatively expensive micrococcal nuclease required according to the procedures in [l-3].…”

Section: Introductionmentioning

confidence: 99%

Excess of micrococcal nuclease may harm the exogenous mRNA in the mRNA‐dependent rabbit reticulocyte cell‐free system

Neeleman

Vloten-Doting

1978

FEBS Letters

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A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus

Cited by 207 publications

References 21 publications

Localization of Genetic Information Involved in the Replication of Alfalfa Mosaic Virus

Localization of Genetic Information Involved in the Replication of Alfalfa Mosaic Virus

Alfalfa mosaic virus RNA3 mutants do not replicate in transgenic plants expressing RNA3-specific genes

Excess of micrococcal nuclease may harm the exogenous mRNA in the mRNA‐dependent rabbit reticulocyte cell‐free system

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