2022
DOI: 10.1002/jev2.12207
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A functional corona around extracellular vesicles enhances angiogenesis, skin regeneration and immunomodulation

Abstract: Nanoparticles can acquire a plasma protein corona defining their biological identity. Corona functions were previously considered for cell-derived extracellular vesicles (EVs). Here we demonstrate that nano-sized EVs from therapy-grade human placental-expanded (PLX) stromal cells are surrounded by an imageable and functional protein corona when enriched with permissive technology. Scalable EV separation from cell-secreted soluble factors via tangential flow-filtration (TFF) and subtractive tandem mass-tag (TMT… Show more

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Cited by 118 publications
(123 citation statements)
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“…Of interest, a very recent study has shown that EVs loaded with Smad2/3-siRNA from subcutaneous adipose tissue stem cells and bone marrow MSCs ameliorate vascular dysfunction and regress inflammation-mediated atherosclerosis [ 329 ]. Finally, EVs from human placental-expanded stromal cells bear a functional corona with immunomodulatory and angiogenic effects with potential application for peripheral artery disease [ 330 ].…”
Section: Extracellular Vesicles As Therapeutic Toolsmentioning
confidence: 99%
“…Of interest, a very recent study has shown that EVs loaded with Smad2/3-siRNA from subcutaneous adipose tissue stem cells and bone marrow MSCs ameliorate vascular dysfunction and regress inflammation-mediated atherosclerosis [ 329 ]. Finally, EVs from human placental-expanded stromal cells bear a functional corona with immunomodulatory and angiogenic effects with potential application for peripheral artery disease [ 330 ].…”
Section: Extracellular Vesicles As Therapeutic Toolsmentioning
confidence: 99%
“…Indeed, as demonstrated for synthetic nanoparticles (Busatto et al, 2019), EVs are able to recruit on their surface numerous proteins which are present in circulation. The nature of such "protein corona" has recently started to be investigated as for other biogenic nanoparticles (Asztalos et al, 2011;Buzas et al, 2018;Paolini, Orizio et al, 2018;Toth et al, 2021;Wolf et al, 2022;Yerneni et al, 2021). Such investigation promises to disclose new properties of EVs since EV protein corona might be involved in EV-mediated cellular communication or can even provide EVs with new regulatory functions.…”
Section:  Discussionmentioning
confidence: 99%
“…Highly purifying EV separation methods always result in lower EV yield. On the other hand, although impurities may affect the functional activity of EVs, the protein coronae around EVs have been found to enhance the therapeutic efficacy of the EVs, especially for regenerative or immunomodulatory purposes [ 233 , 234 , 235 ]. Hence, the EV enrichment method must be chosen wisely according to the therapeutic use.…”
Section: Translation Of Ev-based Therapeuticsmentioning
confidence: 99%