A fully reversible 25-hydroxy steroid kinase involved in oxygen-independent cholesterol side-chain oxidation

Abstract

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Inside Cover: Stable Isotope Phosphate Labelling of Diverse Metabolites is Enabled by a Family of 18O‐Phosphoramidites (Angew. Chem. Int. Ed. 5/2022)

“…[26] Furthermore, several proteomics-based assays with 18 O x -ATP (x = 2-4) were developed, identifying substrates of specific kinases of interest. [27][28][29][30][31] These various applications underline the tremendous potential of 18 O-labelled phosphates to serve as tool compounds for addressing chemical and biological questions. Notably, most methods rely on synthetically well-studied 18 O x -ATP as a probe.…”

Genes and enzymes involved in the biodegradation of the quaternary carbon compound pivalate in the denitrifying Thauera humireducens strain PIV‐1

“…Under anoxic conditions, the current knowledge of quaternary carbon compound degradation pathways is limited to studies with denitrifying bacteria that degrade quaternary carbon-containing steroids such as androgens or cholesterol. During degradation of the latter, the water-dependent hydroxylation of an unactivated primary carbon at the isoprenoid is achieved by an enzyme cascade involving a tertiary alcohol intermediate (Dermer and Fuchs, 2012;Jacoby et al, 2018), and its ATPdependent conversion to an allylic alcohol (Wang et al, 2013;Jacoby et al, 2020a;Jacoby et al, 2021). This cascade allows for the oxygen-independent hydroxylation of an unactivated methyl group next to a tertiary carbon; however, it is not applicable to the quaternary carbons at C10 and C13 of the androstane skeleton (Warnke et al, 2017).…”

Stable Isotope Phosphate Labelling of Diverse Metabolites is Enabled by a Family of 18O‐Phosphoramidites**

“…demonstrated the application of γ‐ 18 O 4 ‐ATP in nucleo to follow protein phosphorylation rates [26] . Furthermore, several proteomics‐based assays with 18 O x ‐ATP ( x =2–4) were developed, identifying substrates of specific kinases of interest [27–31] …”

“…[26] Furthermore,several proteomics-based assays with 18 O x -ATP (x = 2-4) were developed, identifying substrates of specific kinases of interest. [27][28][29][30][31] These various applications underline the tremendous potential of 18 O-labelled phosphates to serve as tool compounds for addressing chemical and biological questions. Notably,most methods rely on synthetically well-studied 18 O x -ATPa sap robe.O ther 18 O-labelled phosphates or phosphoanhydrides are scarce.T he high potential of 18 O-labelled phosphates beyond 18 O-ATP is insufficiently exploited in large part because of a" dearth of synthetic methods".…”

“… [26] Furthermore, several proteomics‐based assays with 18 O x ‐ATP ( x =2–4) were developed, identifying substrates of specific kinases of interest. [ 27 , 28 , 29 , 30 , 31 ]…”