A fully multiplexed CMOS biochip for DNA analysis

Swanson, Paul N.; Gelbart, Richard; Atlas, E.L.; Li, Yang; Grogan, Tammy; Butler, William F.; Ackley, D. E.; Sheldon, Edward L.

doi:10.1016/s0925-4005(99)00478-5

Cited by 51 publications

(33 citation statements)

References 8 publications

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“…Further, this technology allows many advantages over systems that discriminate among simple sequence repeat alleles by size. A chip-based hybridization assay is highly amenable to miniaturization and easily compatible within a device that could also include DNA purification and amplification units, thereby providing a high degree of portability (16,17). This method also provides significant advantages in speed and convenience by eliminating the need for preparing and running sieving gels.…”

Section: Discussionmentioning

confidence: 99%

Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip

Radtkey

Feng²,

Muralhidar³

et al. 2000

Nucleic Acids Research

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We describe a method for the discrimination of short tandem repeat (STR) alleles based on active microarray hybridization. An essential factor in this method is electronic hybridization of the target DNA, at high stringency, in <5 min. High stringency is critical to avoid slippage of hybrids along repeat tracts at allelespecific test sites in the array. These conditions are attainable only with hybridization kinetics realized by electronic concentration of DNA. A sandwich hybrid is assembled, in which proper base stacking of juxtaposed terminal nucleotides results in a thermodynamically favored complex. The increased stability of this complex relative to non-stacked termini and/or base pair mismatches is used to determine the identification of STR alleles. This method is capable of simultaneous and precise identification of alleles containing different numbers of repeats, as well as mutations within these repeats. Given the throughput capabilities of microarrays our system has the potential to enhance the use of microsatellites in forensic criminology, diagnostics and genetic mapping.

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Section: Discussionmentioning

confidence: 99%

Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip

Radtkey

Feng²,

Muralhidar³

et al. 2000

Nucleic Acids Research

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“…protein assay) recognition processes using different surface modification methods. In another study, Swanson and colleagues reported a multiplexed CMOS sensor chip-based DNA hybridization and sequencing method [73]. Herein, CMOS chip was modified with 400 or more reaction sites, and each site was controlled by a hardware and software electronic circuit called a field-programmable gate array (FPGA).…”

Section: Cmos Sensor-based Dna Amplification and Gene Sequencingmentioning

confidence: 99%

Overview of CMOS image sensor use in molecular diagnostics

Devadhasan

Yoo

Kim

2015

Current Applied Physics

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“…Electronic assembly of DNA microarrays on active silicon chips has been demonstrated by several groups [48,55]. Since DNA molecules are negatively charged, they can be attracted to positively biased test sites.…”

Section: Dna Manipulation and Detection With Microelectronic Chipsmentioning

confidence: 99%

“…Electronically controlled on-chip in situ DNA construction has also successfully been demonstrated [56]. Moreover, in [55], electronics is further leveraged to achieve a stringency control. After hybridization, a repulsive force is applied by negatively biasing the test site, so that only perfectly matching strands are retained.…”

Section: Dna Manipulation and Detection With Microelectronic Chipsmentioning

confidence: 99%

Microelectronic Chips for Molecular and Cell Biology

et al. 2003

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The development of microfabricated devices manufactured in silicon, glass, or plastic materials is a well-known trend in the research of novel biological techniques and tools over the last two decades, resulting in a multitude of start-up companies serving the pharmaceutical, biotechnology, and diagnostics markets. However, the idea of implementing such devices on microelectronic substrates has been introduced only recently. This chapter aims to describe the state-of-the-art of microsystems for molecular and cell biology produced in general purpose CMOS (complementary metal oxide semiconductor) technology, emphasizing the advantages of this approach along with their challenges and limitations. This chapter discusses significant examples of fully tested devices in comparison with existing state-of-the-art techniques.

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A fully multiplexed CMOS biochip for DNA analysis

Cited by 51 publications

References 8 publications

Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip

Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip

Overview of CMOS image sensor use in molecular diagnostics

Microelectronic Chips for Molecular and Cell Biology

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