A fully integrated rapid on-chip antibiotic susceptibility test – A case study for Mycobacterium smegmatis

Ghorbanpoor, Hamed; Dizaji, Araz Norouz; Akcakoca, Iremnur; Blair, Ewen O.; Öztürk, Yasin; Hoskisson, Paul A.; Kocagoz, Tanil; Avcı, Hüseyin; Corrigan, Damion K.; Güzel, Fatma Doğan

doi:10.1016/j.sna.2022.113515

Cited by 9 publications

(7 citation statements)

References 70 publications

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“…44 Several recent studies have demonstrated impressive LOD, ranging from 2.8 to 10 fM, and even reaching up to 10 CFU mL −1 . These achievements were realized by utilizing DNA linear probes with biotin-labeled reporters, 45 microfluidic chip devices, 46 or nanocomposites based on molybdenum disulfide, 47 with detection times spanning between 80 minutes to 4 hours. While these outcomes are noteworthy, there is room for improvement by employing stem-loop probes.…”

Section: Resultsmentioning

confidence: 99%

DNA nanotechnology for nucleic acid analysis: sensing of nucleic acids with DNA junction-probes

Foguel,

Zamora,

Ojeda

et al. 2024

Analyst

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Section: Resultsmentioning

confidence: 99%

DNA nanotechnology for nucleic acid analysis: sensing of nucleic acids with DNA junction-probes

Foguel,

Zamora,

Ojeda

et al. 2024

Analyst

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“…In our previous study, we successfully performed AST assays for MSM after 1-day incubation, due to the much more rapid growth of this organism. 29,43 To specify the sensitivity of the proposed sensor, we examined EIS changes for different concentrations of TB (10 8 -10 3 ). The results demonstrate that the applied sensor platform detects TBs in the 10 8 -10 3 CFU/mL range with a significant trend (Figure 3a).…”

Section: Resultsmentioning

confidence: 99%

“…TB AST assays were, therefore, evaluated on the fourth day (96 h) post‐incubation. In our previous study, we successfully performed AST assays for MSM after 1‐day incubation, due to the much more rapid growth of this organism 29,43 …”

Section: Resultsmentioning

confidence: 99%

Simple and low‐cost antibiotic susceptibility testing for Mycobacterium tuberculosis using screen‐printed electrodes

Ghorbanpoor

Akcakoca

Dizaji

et al. 2023

Biotech and App Biochem

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One quarter of the global population is thought to be latently infected by Mycobacterium tuberculosis (TB) with it estimated that 1 in 10 of those people will go on to develop active disease. Due to the fact that M. tuberculosis (TB) is a disease most often associated with low‐ and middle‐income countries, it is critical that low‐cost and easy‐to‐use technological solutions are developed, which can have a direct impact on diagnosis and prescribing practice for TB. One area where intervention could be particularly useful is antibiotic susceptibility testing (AST). This work presents a low‐cost, simple‐to‐use AST sensor that can detect drug susceptibility on the basis of changing RNA abundance for the typically slow‐growing M. tuberculosis (TB) pathogen in 96 h using screen‐printed electrodes and standard molecular biology laboratory reactionware. In order to find out the sensitivity of applied sensor platform, a different concentration (108–103 CFU/mL) of M. tuberculosis was performed, and limit of detection and limit of quantitation were calculated as 103.82 and 1011.59 CFU/mL, respectively. The results display that it was possible to detect TB sequences and distinguish antibiotic‐treated cells from untreated cells with a label‐free molecular detection. These findings pave the way for the development of a comprehensive, low‐cost, and simple‐to‐use AST system for prescribing in TB and multidrug‐resistant tuberculosis.

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“…After thermal lysis, the samples were transferred into adjacent chambers where the resulting nucleic acid levels in response to the specific incubation conditions were measured via integrated Au microelectrodes. 232 A microfluidic PDMS chip for real-time monitoring of the growth dynamics and phenotypic alterations of M. smegmatis has been proposed as a tool for investigating drug-induced stress with single cell resolution. The device was composed of arrays of 0.9 μm high chambers for bacteria trapping and culture under different conditions, including drug exposure.…”

Section: Tuberculosismentioning

confidence: 99%

Microfluidic systems for infectious disease diagnostics

Lehnert,

Gijs

2024

Lab Chip

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A fully integrated rapid on-chip antibiotic susceptibility test – A case study for Mycobacterium smegmatis

Cited by 9 publications

References 70 publications

DNA nanotechnology for nucleic acid analysis: sensing of nucleic acids with DNA junction-probes

DNA nanotechnology for nucleic acid analysis: sensing of nucleic acids with DNA junction-probes

Simple and low‐cost antibiotic susceptibility testing for Mycobacterium tuberculosis using screen‐printed electrodes

Microfluidic systems for infectious disease diagnostics

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