A fully integrated microdevice for capturing, amplification, and colorimetric detection of foodborne pathogens

La, Hoang Chau; Lee, Nae Yoon

doi:10.1007/s00542-020-04877-8

Cited by 1 publication

(2 citation statements)

References 35 publications

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“…Important features of these devices include their portability, limit of detection, and time for analysis. To achieve high selectivity, some of these methods require extraction and amplification of nucleic acids, which adds a level of complexity to the devices. , Another biosensor, based on surface-enhanced Raman scattering, utilized tags consisting of gold nanorods decorated with both aptamers and Raman reporters . These tags were used in combination with antibody-conjugated nanoparticles for the selective detection and quantification of both E. coli O157:H7 and Salmonella typhimurium , with LODs of <10 CFU/mL.…”

Section: Discussionmentioning

confidence: 99%

“…Although detectable signals can be generated within a short time frame, the tests are laborious, expensive, and prone to yield false positive results . As an alternative to conventional methods, novel biosensors are being developed. − An important component of these biosensors, which impacts both selectivity and sensitivity, is the recognition element that can interact with the analyte of interest, i.e., pathogenic bacteria …”

Section: Introductionmentioning

confidence: 99%

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Immobilized Reporter Phage on Electrospun Polymer Fibers for Improved Capture and Detection of Escherichia coli O157:H7

Chen

Harrison

et al. 2021

ACS Food Sci. Technol.

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Escherichia coli O157:H7 is a foodborne Shiga-toxin-producing bacterium that leads to millions of cases of illness every year. The development of a portable biosensor that rapidly detects this pathogen can greatly reduce both the time required to identify the source of contamination within the food production chain and the scale of outbreaks. Here we propose a swab for the detection of E. coli O157:H7 made from electrospun poly-3-hydroxybutyrate (PHB) on which the reporter bacteriophage (phage), PP01engineered to express the bioluminescent protein Nanoluc upon infection of the pathogenhas been immobilized. In the development of the material, the properties of phage-immobilized electrospun porous, nonwoven PHB mats were compared to those of phage-immobilized flat PHB films produced by solvent casting. Dynamics of E. coli infections initiated with the phage-immobilized materials showed the electrospun mats had the highest infectivity, indicative of higher phage surface density (confirmed by microscopy) and activitytwo important properties for the sensitivity and response time of biosensors. In sample tests, successful detection of E. coli O157:H7 cells in both milk and Tryptic Soy Broth at concentrations of 105 CFU/mL within 1 h and 101 CFU/mL within 3 h demonstrates the promising potential of the system as a food safety assessment tool.

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Section: Discussionmentioning

confidence: 99%