A fully integrated distance readout ELISA-Chip for point-of-care testing with sample-in-answer-out capability

Liu, Dan; Li, Xingrui; Zhou, Jing; Liu, Shibo; Tian, Tian; Song, Yanling; Zhu, Zhi; Zhou, Lili; Ji, Tao; Yang, Chaoyong

doi:10.1016/j.bios.2017.04.044

Cited by 99 publications

(49 citation statements)

References 25 publications

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“…The field of integrated microfluidic biomarker analysis has been growing rapidly. Just in recent years, new microfluidic platforms for biomarker analysis have emerged with capabilities for colorimetric or electrochemical assays , ELISA , and other biosensors . However, a common disadvantage of most of these studies is that they were only capable of analyzing a single analyte per device.…”

Section: Introductionmentioning

confidence: 99%

Microchip electrophoresis separation of a panel of preterm birth biomarkers

Nielsen

Sonker

et al. 2018

Electrophoresis

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Preterm birth (PTB) is responsible for over one million infant deaths annually worldwide. Often, the first and only indication of PTB risk is the onset of early labor. Thus, there is an urgent need for an early PTB risk diagnostic that is inexpensive, reliable, and robust. Here, we describe the development of a microchip electrophoresis (μCE) method for separating a mixture of six PTB protein and peptide biomarkers present in maternal blood serum. μCE devices were photografted with a poly(ethylene glycol) diacrylate surface coating to regulate EOF and reduce nonspecific analyte adsorption. Separation conditions including buffer pH, buffer concentration, and applied electric field were varied to improve biomarker peak resolution while minimizing deleterious effects like Joule heating. In this way, it was possible to separate six PTB biomarkers, the first μCE separation of this biomarker panel. LODs were also measured for each of the six PTB biomarkers. In the future, this μCE separation can be integrated with upstream maternal blood serum sample preparation steps to yield a complete PTB risk diagnosis microdevice.

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Section: Introductionmentioning

confidence: 99%

Microchip electrophoresis separation of a panel of preterm birth biomarkers

Nielsen

Sonker

et al. 2018

Electrophoresis

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“…This turnaround time is much shorter than conventional ELISA, which normally takes 6 h to overnight, 19 and comparable to some point-of-care ELISA chips reported. 49…”

Section: Resultsmentioning

confidence: 99%

Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays

Yang

Zeng

2018

Lab Chip

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Rapid, sensitive analysis of protein biomarkers is of tremendous biological and clinical significance. Immunoassay is a workhorse tool for protein analysis and has been under continuous investigations to develop new methods and to improve the analytical performance. Herein we report a pneumatically gated microfluidic communicating vessel (μCOVE) chip for rapid and sensitive immunomagnetic ELISA. A distinct feature of our device is that it employs the communicating vessel principle as a simple means to generate fast transient hydrodynamic flow to enable effective flow washing without the need of excessive incubation, which greatly simplifies and expedites the assay workflow, compared to conventional microfluidic flow-based immunoassays. The stationary multi-phase microfluidic techniques have been developed for fast bead washing. However, they have some limitations, such as the need of careful control of interfacial properties, large bead quantity required for reliable interphase bead transport, and relatively high bead loss during surface tension-gated traverse. Our signal-phase μCOVE chip can overcome such limitations and facilitate the manipulation of magnetic beads to streamline the assay workflow. We showed that the μCOVE device affords highly sensitive quantification of CEA and EGFR proteins with the LOD down to the sub-picogram per mL level. Direct detection of EGFR in the crude A431 cell lysate was also demonstrated to further validate the ability of our device for rapid and quantitative analysis of complex biological samples. Overall, our work presents a unique platform that combines the merits of the stationary multi-phase systems and the flow-based microfluidics. This novel immunoassay microsystem holds promising potential for a broad range of biological and clinical applications, owing to its simplicity and high performance.

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“…mAb1-Au@PtNPs were prepared according to our previously published work [23]. Briefly, 10 μL of 1% (wt%) Tween 20 and 5 μL of 100 μM biotin-mPEG-SH were added to 1 mL Au@PtNPs.…”

Section: Methodsmentioning

confidence: 99%

Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips

et al. 2019

J Nanobiotechnol

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Background Immunochromatographic strips (ICSs) are a practical tool commonly used in point-of-care testing (POCT) applications. However, ICSs that are currently available have low sensitivity and require expensive equipment for quantitative analysis. These limitations prohibit their extensive use in areas where medical resources are scarce. Methods We developed a novel POCT platform by integrating a gas generation biosensor with Au@Pt Core/Shell nanoparticle (Au@PtNPs)-based ICSs (G-ICSs). The resulting G-ICSs enabled the convenient and quantitative assessment of a target protein using the naked eye, without the need for auxiliary equipment or complicated computation. To assess this platform, C-reactive protein (CRP), a biomarker commonly used for the diagnosis of acute, infectious diseases was chosen as a proof-of-concept test. Results The linear detection range (LDR) of the G-ICSs for CRP was 0.05–6.25 μg/L with a limit of detection (LOD) of 0.041 μg/L. The G-ICSs had higher sensitivity and wider LDR when compared with commonly used AuNPs and fluorescent-based ICSs. When compared with results from a chemiluminescent immunoassay, G-ICS concordance rates for CRP detection in serum samples ranged from 93.72 to 110.99%. Conclusions These results demonstrated that G-ICSs have wide applicability in family diagnosis and community medical institutions, especially in areas with poor medical resources. Electronic supplementary material The online version of this article (10.1186/s12951-019-0493-z) contains supplementary material, which is available to authorized users.

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A fully integrated distance readout ELISA-Chip for point-of-care testing with sample-in-answer-out capability

Cited by 99 publications

References 25 publications

Microchip electrophoresis separation of a panel of preterm birth biomarkers

Microchip electrophoresis separation of a panel of preterm birth biomarkers

Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays

Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips

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