A fully electronic sensor for the measurement of cDNA hybridization kinetics

Bandiera, L.; Cellere, G.; Cagnin, Stefano; Toni, A. De; Zanoni, Enrico; Lanfranchi, Gerolamo; Lorenzelli, Leandro

doi:10.1016/j.bios.2006.09.025

Cited by 28 publications

(13 citation statements)

References 28 publications

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“…Direct functionalization by covalent attachment of a self-assembled monolayer of DNA probes to the sensor surface (either gold or oxide based) is most commonly used [11,16,17,71,72]. Nonetheless, electrostatic immobilization of probe DNA has also been described [15,22,73,74]. …”

Section: Applications Of Field Effect Biosensorsmentioning

confidence: 99%

Field Effect Sensors for Nucleic Acid Detection: Recent Advances and Future Perspectives

Veigas

Fortunato

Baptista

2015

Sensors

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In the last decade the use of field-effect-based devices has become a basic structural element in a new generation of biosensors that allow label-free DNA analysis. In particular, ion sensitive field effect transistors (FET) are the basis for the development of radical new approaches for the specific detection and characterization of DNA due to FETs’ greater signal-to-noise ratio, fast measurement capabilities, and possibility to be included in portable instrumentation. Reliable molecular characterization of DNA and/or RNA is vital for disease diagnostics and to follow up alterations in gene expression profiles. FET biosensors may become a relevant tool for molecular diagnostics and at point-of-care. The development of these devices and strategies should be carefully designed, as biomolecular recognition and detection events must occur within the Debye length. This limitation is sometimes considered to be fundamental for FET devices and considerable efforts have been made to develop better architectures. Herein we review the use of field effect sensors for nucleic acid detection strategies—from production and functionalization to integration in molecular diagnostics platforms, with special focus on those that have made their way into the diagnostics lab.

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Section: Applications Of Field Effect Biosensorsmentioning

confidence: 99%

Field Effect Sensors for Nucleic Acid Detection: Recent Advances and Future Perspectives

Veigas

Fortunato

Baptista

2015

Sensors

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show abstract

“…With the recent advance in microelectronic technology, ISFET-based sensing mechanism plays an important role in the development of microsensors mainly due to the features of low cost, ease of miniaturization, as well as low output impedance (Bergveld, 2003). Borrowing from the wellestablished ISFET sensing mechanism, pH-ISFET-based biosensors has been actively proposed for various biosensing purposes such as glucose (Luo et al, 2004), lactate (Kharitonov et al, 2001), creatinine (Premanode and Toumazou, 2007), urea (Boubriak et al, 1995) or DNA (Bandiera et al, 2007). The fundamental configuration of biosensing of these kinds is based on the conjugation of a pH sensitive electrode with a biological recognition layer normally containing enzymes specific to the detection target.…”

Section: Introductionmentioning

confidence: 99%

Structural properties and sensing performance of high-k Nd2TiO5 thin layer-based electrolyte–insulator–semiconductor for pH detection and urea biosensing

Pan

Lin

et al. 2009

Biosensors and Bioelectronics

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“…Detection of the complementary 12-mer strand was attained with a detection limit of 1 nmol/L and resolution of single-base mismatches was also demonstrated. Similar work was demonstrated in real-time complete selective hybridization of cDNA with either 20-mer or 400-mer probe DNA and showed that the longer DNA chains had slower hybridization kinetics (Bandiera et al , 2007). Other workers utilized 3-aminopropyldimethylethoxysilane to immobilize probe DNA, demonstrating the detection of natural 19-mer sequences (Uslu et al , 2004), and so proposed that well-defi ned surface chemistries were essential for sensitivity and reproducibility.…”

Section: Label-free Electrochemical Detection Of Dnamentioning

confidence: 88%