A Fully Automated Sequential‐Injection Analyser for Dual Electrogenerated Chemiluminescence/Amperometric Detection

Abstract

This work describes the development of a dedicated, fully automated sequential-injection analysis (SIA) apparatus suitable for simultaneous electrogenerated chemiluminescence (ECL) and amperometric detection. The instrument is composed of a peristaltic pump, a multiposition selection valve, a home-made potentiostat, a thin-layer electrochemical/optical flow-through cell, and a light detector. Control of the experimental sequence and simultaneous data acquisition of the light and the current intensities were performed in LabVIEW6.1. The CL reagents and the sample were first aspirated as distinct zones into the holding coil of the analyser and, then, delivered to the cell; during their travel, the individual zones mixed and the ECL reaction was initiated as soon as the mixed regents reached the cell. The utility of the analyser was demonstrated for the detection of oxalate and H 2 O 2 based on their ECL reactions with Ru ( bpy ) 3 2 and luminol, respectively.

Electrogenerated Chemiluminescence and Its Biorelated Applications

“…Wilson et al , recently reported a flow injection electrochemiluminometer either with (Figure ) or without a magnet in the flow cell for enzyme immunoassays of explosives 2,4,6-trinitrotoluene (TNT) and pentaerthyritol tetranitrate (PETN). Also, a fully automated sequential injection analyzer for simultaneous ECL and amperometric detection has been described . The instrument is composed of a peristaltic pump, a multiposition selection valve, a homemade potentiostat, a thin-layer electrochemical/optical flow-through cell, and a light detector (Figure ).…”

“…(b) Schematic diagram of the electrochemical/optical flow-through cell. Reprinted with permission from ref. 2006 Hindawi.…”

“…Also, a fully automated sequential injection analyzer for simultaneous ECL and amperometric detection has been described. 292 The instrument is composed of a peristaltic pump, a multiposition selection valve, a homemade potentiostat, a thin-layer electrochemical/optical flow-through cell, and a light detector (Figure 22).…”

A LabVIEW®-based software for the control of the AUTORAD platform: a fully automated multisequential flow injection analysis Lab-on-Valve (MSFIA-LOV) system for radiochemical analysis

“…In recent years, published papers describe such LabVIEW ® -based software for the automation of specific analytical systems such as flow injection analysis (FIA) [ 8 ], sequential injection analysis (SIA) [ 9 ] or capillary electrophoresis (CE) [ 1 ]. Applications include automatic liquid handling, treatment, separation and, in some cases, detection of the interest analyte.…”