A full micro-fluidic system for single oocyte manipulation including an optical sensor for cell maturity estimation and fertilisation indication

Zeggari, Rabah; Wacogne, Bruno; Pieralli, Christian; Roux, Christophe; Gharbi, Tijani

doi:10.1016/j.snb.2007.02.045

Cited by 31 publications

(30 citation statements)

References 14 publications

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“…Although manual manipulation is used in this paper, our ulterior motive is to integrate the device in an automated labon-chip. This work is in line with our researches concerning labs-on-chips devoted to the qualification of human oocytes (Sadani et al 2005;Zeggari et al 2006Zeggari et al , 2007. The system consists of an SU-8 micro-beam which acts as a force sensor.…”

Section: Introductionsupporting

confidence: 76%

Measuring the mechanical behaviour of human oocytes with a very simple SU-8 micro-tool

Wacogne

Pieralli

Roux

et al. 2007

Biomed Microdevices

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The elasticity of human oocytes has been estimated by means of a very simple SU-8 microsystem. The device consists of a double SU-8 layer spin coated on a silicon wafer. A micro-beam is patterned by standard photolithography. The silicon wafer is back etched using deep reactive ion etching (DRIE) in order to release the micro-beam. Because the SU-8 resin exhibits a very low Young modulus, beams with stiffnesses of 12 N/m have been easily fabricated. Although the stiffness is not as low as that of Atomic Force Microscopes cantilevers, it was possible to estimate the "deformation-load" characteristics of a few oocytes. A "flattening parameter" of the oocytes is proposed as a function of the applied load and experimental values range from 0.86 microm(2)/Pa to 3.4 microm(2)/Pa for the oocytes under test. Note that this paper belongs to the very few communications concerning the mechanical behaviour of human oocytes.

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Section: Introductionsupporting

confidence: 76%

Measuring the mechanical behaviour of human oocytes with a very simple SU-8 micro-tool

Wacogne

Pieralli

Roux

et al. 2007

Biomed Microdevices

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“…To be effective, this new technology should be fully compatible with physician's protocols and constraints, but should also provide analysis methods, preferably on a multimodal basis, to improve the reliability of oocyte maturity diagnosis. Both mechanical and optical approaches are thought effective to establish a reliable diagnosis despite the variability of the oocytes' evolution [28,29]. The mechanical tests will provide the stiffness determination of oocytes for different loads.…”

Section: Discussionmentioning

confidence: 99%

Experimental measurement of human oocyte mechanical properties on a micro and nanoforce sensing platform based on magnetic springs

Abadie

Roux

Piat

et al. 2014

Sensors and Actuators B: Chemical

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This article presents a new micro and nanoforce sensor used to perform a mechanical characterisation of human oocytes. This device is based on the use of low-stiffness magnetic springs. The oocytes to be characterised are placed on a force-sensitive platform. A manipulator equipped with a standard micropipette is used to mechanically compress the oocyte. Some complete "force-compression length" curves associated with mechanical load-unload cycles are given. These curves show the linear, the non-linear and also the plastic mechanical behaviour of the oocytes. These characterisations must be considered as a preliminary result which illustrates that the mechanical variability and the mechanical evolution of human oocytes during their maturation process can be observed with a force sensor based on magnetic springs.

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“…Because of the high cytoplasmic lipid content in porcine embryos, the light transmission is very low, especially in the near infrared wavelength range. 24 Therefore, the fluence threshold for fusion of two-cell porcine embryos was 3-to 4-fold higher (∼ 180 to 280 mJ/cm 2 resp. 330 to 520 mW).…”

Section: Fusion Of Parthenogenetic Two-cell Porcine Embryosmentioning

confidence: 93%

Femtosecond laser-induced fusion of nonadherent cells and two-cell porcine embryos

et al. 2011

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Abstract. Cell fusion is a fundamental biological process that can be artificially induced by different methods. Although femtosecond (fs) lasers have been successfully employed for cell fusion over the past few years, the underlying mechanisms are still unknown. In our experimental study, we investigated the correlation between fs laser-induced cell fusion and membrane perforation, and the influence of laser parameters on the fusion efficiency of nonadherent HL-60 cells. We found that shorter exposure times resulted in higher fusion efficiencies with a maximum of 21% at 10 ms and 100 mJ/cm 2 (190 mW). Successful cell fusion was indicated by the formation of a long-lasting vapor bubble in the irradiated area with an average diameter much larger than in cell perforation experiments. With this knowledge, we demonstrated, for the first time, the fusion of very large parthenogenetic two-cell porcine embryos with high efficiencies of 55% at 20 ms and 360 mJ/cm 2 (670 mW). Long-term viability of fused embryos was proven by successful development up to the blastocyst stage in 70% of cases with no significant difference to controls. In contrast to previous studies, our results indicate that fs laser-induced cell fusion occurs when the membrane pore size exceeds a critical value, preventing immediate membrane resealing.C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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A full micro-fluidic system for single oocyte manipulation including an optical sensor for cell maturity estimation and fertilisation indication

Cited by 31 publications

References 14 publications

Measuring the mechanical behaviour of human oocytes with a very simple SU-8 micro-tool

Measuring the mechanical behaviour of human oocytes with a very simple SU-8 micro-tool

Experimental measurement of human oocyte mechanical properties on a micro and nanoforce sensing platform based on magnetic springs

Femtosecond laser-induced fusion of nonadherent cells and two-cell porcine embryos

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