A fruitful alliance: the synergy between<i>Atopobium vaginae</i>and<i>Gardnerella vaginalis</i>in bacterial vaginosis-associated biofilm

Hardy, Liselotte; Jespers, Vicky; Abdellati, Saïd; Baetselier, Irith De; Mwambarangwe, Lambert; Musengamana, Viateur; Wijgert, Janneke van de; Vaneechoutte, Mario; Crucitti, Tania

doi:10.1136/sextrans-2015-052475

Cited by 86 publications

(79 citation statements)

References 25 publications

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“…BV is characterized by a change in the microbial composition of the vaginal ecosystem where the prevailing Lactobacillus spp., associated with an optimal vaginal microbiota, are outnumbered by other microorganisms, including species of the genus Gardnerella and Atopobium vaginae (Ferris et al, 2004;Verhelst et al, 2004;dos Santos Santiago et al, 2012;Jung et al, 2017;Muzny et al, 2019). Noteworthy, the involvement of A. vaginae in BV rarely occurs in the absence of Gardnerella (Bradshaw et al, 2006;Hardy et al, 2015Hardy et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

“…Evidence of a possible dependent relationship between Gardnerella spp. and A. vaginae has been demonstrated on BV-associated biofilms (Swidsinski et al, 2005;Hardy et al, 2015Hardy et al, , 2016. Nevertheless, as biological interactions in BV-associated biofilms are still poorly understood, we aimed to analyze the interactions between G. vaginalis and A. vaginae, using a previously described in vitro dual-species biofilm model .…”

Section: Introductionmentioning

confidence: 99%

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Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model

Castro¹,

Roșca²,

Cools

et al. 2020

Front. Cell. Infect. Microbiol.

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Bacterial vaginosis (BV) is the most common vaginal infection among women of reproductive age. A hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by facultative anaerobes of the genus Gardnerella, which then becomes a scaffold for other species to adhere to. One of the species often found incorporated in Gardnerella mediated biofilms is Atopobium vaginae. Interestingly, A. vaginae is very rarely found without the presence of Gardnerella. However, not much is known regarding the interactions between A. vaginae and Gardnerella species. This study assessed biological interactions between Gardnerella vaginalis and A. vaginae. In our in vitro model, by using specific Gardnerella and A. vaginae Peptide Nucleic Acid (PNA)-Fluorescence In Situ Hybridization (FISH) probes, we confirmed that A. vaginae was able to incorporate a pre-formed G. vaginalis biofilm, accounting for up to 20% of the total number of biofilm cells. However, our findings showed that almost 92% of A. vaginae cells lost viability after 48 h of mono-species planktonic growth, but were able to maintain viability when co-cultured with Gardnerella or after pre-conditioning with cell-free supernatant of Gardnerella cultures. While the in vitro conditions are very different from the in vivo microenvironment, this study contributes to a better understanding of why A. vaginae vaginal colonization rarely occurs in the absence of Gardnerella. Overall, this highlights the importance of microbial interactions between BV-associated bacteria and demands more studies focused on the polymicrobial bacterial communities found in BV.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model

Castro¹,

Roșca²,

Cools

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Furthermore, while L. iners has a lower protective value than the other Lactobacillus [36], the negative edge between L. iners and Atopobium suggests a relationship related to environment protection. Also, the positive edge from Atopobium to Gardnerella is supported by the synergy observed between these two taxa in bacterial vaginosis [37]. Finally, it is important to note that the shifts and composition of the vaginal microbiome vary considerably between each subject.…”

Section: Discussionmentioning

confidence: 99%

Dynamic interaction network inference from longitudinal microbiome data

Lugo-Martinez

Ruiz-Perez

Narasimhan

et al. 2018

Preprint

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Background Several studies have focused on the microbiota living in environmental niches including human body sites. In many of these studies researchers collect longitudinal data with the goal of understanding not just the composition of the microbiome but also the interactions between the different taxa. However, analysis of such data is challenging and very few methods have been developed to reconstruct dynamic models from time series microbiome data. Results Here we present a computational pipeline that enables the integration of data across individuals for the reconstruction of such models. Our pipeline starts by aligning the data collected for all individuals. The aligned profiles are then used to learn a dynamic Bayesian network which represents causal relationships between taxa and clinical variables. Testing our methods on three longitudinal microbiome data sets we show that our pipeline improve upon prior methods developed for this task. We also discuss the biological insights provided by the models which include several known and novel interactions. Conclusions We propose a computational pipeline for analyzing longitudinal microbiome data. Our results provide evidence that microbiome alignments coupled with dynamic Bayesian networks improve predictive performance over previous methods and enhance our ability to infer biological $ These authors have contributed equally to this work. relationships within the microbiome and between taxa and clinical factors.

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“…Later studies of Swidsinski et al [20] confirmed that 1-40% of the mass of biofilm in the clinical material were made up of Atopobium vaginae. Recent studies of the bacterial vaginosis etiology have shown that Atopobium vaginae is found in microbial associations in 80 % to 95 % of cases [21,22].…”

Section: The Aimmentioning

confidence: 99%

Etiological and pathogenetic aspects of non-specific bacterial vaginosis

Ananieva

2018

ZMJ

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The aim of this review was the biological properties analysis of microorganisms known as the main contributors to the non-specific bacterial vaginosis development.The mechanisms of anaerobic vaginal microflora pathogenic action have been found out to be the main cause of non-specific inflammatory processes. Special attention was given to the genera of Mobilunkus spp., Atopobium spp., Prevotella sp. representatives, which were studied as vaginal flora due to the modern diagnostic methods and techniques development.The homeostasis of the vaginal ecosystem results from complex interactions and synergies among the host and different microorganisms that colonize the vaginal mucosa and healthy vaginal microbiota is generally dominated by Lactobacillus spp. Lactobacilli which have been known to be antagonistic toward a variety of microorganisms.In cases of bacterial vaginoses, the proportion of Lactobacilus spp sharply decreases, while the share of anaerobic microorganisms increases: Gardnerella vaginalis, Atopobium sp., Mobiluncus sp., Prevotella sp. The virulence factors of Gardnerella vaginalis pathogenicity are: its ability to form biofilms; the presence of genes responsible for cytotoxicity.The ability of Gardnerella vaginalis, Atopobium sp., Mobiluncus sp., Prevotella sp. to produce short chain volatile fatty acids isomers results in the vaginal mucosa irritation (itching or burning) and discharge with unpleasant odour in BV associated with these microorganisms.Atopobium vaginae, together with Mobiluncus spp. and Prevotella spp., provoke the accumulation of pro-inflammatory cytokines. The whole mechanism leads to an inflammation worsening in BV and stimulates the chronic process development.Both microorganisms Mobiluncus spp. and A. vaginae are competitive lactobacilli in relation to the glycogen consumption.Conclusions. Analysis of literature shows that pathogens of non-specific bacterial vaginosis have wide range of pathogenicity factors. This provides pathogenesis and clinical signs of the disease.Етіологічні та патогенетичні аспекти неспецифічного бактеріального вагінозу М. М. АнаньєваМета роботи -на основі відомостей фахової літератури виконати аналіз даних щодо біологічних властивостей мікроор-ганізмів, які є основними учасниками неспецифічного бактеріального вагінозу.У результаті розкрито механізми патогенної дії анаеробної вагінальної мікрофлори як основної причини неспецифічних запальних процесів. Особливу увагу приділили представникам родів Mobilunkus spp., Atopobium spp., Prevotella sp., що вивчені як флора піхви завдяки розвитку сучасних методів дослідження.Гомеостаз серед представників бактеріальної мікрофлори в піхві підтримується завдяки антагонізму лактобацил щодо решти представників. У нормі лактобацили становлять 90 % вагінальної мікрофлори. Завдяки виділенню великої кількості кислот, підтримці рН на рівні 3,5-4,4, виділенню бактеріоцинів лактобацили стримують патогенну мікрофлору.При неспецифічних бактеріальних вагінозах різко зменшується частка Lactobacilus spp., збільшується кількість анаероб...

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A fruitful alliance: the synergy betweenAtopobium vaginaeandGardnerella vaginalisin bacterial vaginosis-associated biofilm

Cited by 86 publications

References 25 publications

Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model

Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model

Dynamic interaction network inference from longitudinal microbiome data

Etiological and pathogenetic aspects of non-specific bacterial vaginosis

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