A FRET-based detection of N-acetylneuraminic acid using CdSe/ZnS quantum dot and exonuclease III-assisted recycling amplification strategy

Chen, Jinri; Sun, Nan; Chen, Haohan; Zhang, Yuting; Wang, Xiaoli; Zhou, Nandi

doi:10.1016/j.foodchem.2021.130754

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…Fluorescent nanorods were self-assembled by π - π stacking and hydrophobic effect using 4-(4-(pyren-1-yl) butyramido) phenylboronic acid, and two-photon imaging of sialic acid on the surface of living cells was achieved [ 49 ]. Photoelectrochemical biosensing platform modified by Ag 2 S/AuNP composites on the ITO electrode could nondestructively detect sialic acid on the surface of MCF-7 cells [ 50 ]. Electrochemical cytosensor analyzed sialic acid on the cell surface by boric acid polythiophene with the detection limit of 10 cells/mL [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage

et al. 2022

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Evaluating tumor development is of great importance for clinic treatment and therapy. It has been known that the amounts of sialic acids on tumor cell membrane surface are closely associated with the degree of cancerization of the cell. So, in this work, cellular interface supported CRISPR/Cas trans-cleavage has been explored for electrochemical simultaneous detection of two types of sialic acids, i.e., N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac). Specifically, PbS quantum dot-labeled DNA modified by Neu5Gc antibody is prepared to specifically recognize Neu5Gc on the cell surface, followed by the binding of Neu5Ac through our fabricated CdS quantum dot-labeled DNA modified by Sambucus nigra agglutinin. Subsequently, the activated Cas12a indiscriminately cleaves DNA, resulting in the release of PbS and CdS quantum dots, both of which can be simultaneously detected by anodic stripping voltammetry. Consequently, Neu5Gc and Neu5Ac on cell surface can be quantitatively analyzed with the lowest detection limits of 1.12 cells/mL and 1.25 cells/mL, respectively. Therefore, a ratiometric electrochemical method can be constructed for kinetic study of the expression and hydrolysis of Neu5Gc and Neu5Ac on cell surface, which can be further used as a tool to identify bladder cancer cells at different development stages. Our method to evaluate tumor development is simple and easy to be operated, so it can be potentially applied for the detection of tumor occurrence and development in the future.

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Section: Discussionmentioning

confidence: 99%

Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage

et al. 2022

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“…To date, there is still much biomedical research being conducted using Cd‐based QDs and achieved great performances. [ 19,32,90 ]…”

Section: Challenges For Biomedical Applicationsmentioning

confidence: 99%

“…To date, there is still much biomedical research being conducted using Cd-based QDs and achieved great performances. [19,32,90] In many studies, Cd-and Pb-free QDs have shown much less toxicity and seem to be safe enough for living organisms. [36,91] However, Cd-and Pb-free QDs also have toxicity concerns.…”

Section: Toxicitymentioning

confidence: 99%

“…Chen et al provided a FRET-based probe using CdSe/ZnS QDs quencher dye black hole quencher 2 (BHQ2) for detecting N-acetylneuraminic acid, a potential biomarker for cardiovascular disease and cancer. [90] In this study, an exonuclease III-assisted recycling amplification strategy was designed, and an ultra-low detection limit of 24 fm was realized. While some other biosensors can still yield low detection limits without the use of specific amplification strategies.…”

Section: Detection Of Biomarkersmentioning

confidence: 99%

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Recent Advances in Metal Chalcogenide Quantum Dots: From Material Design to Biomedical Applications

Piao

Yang

Cui

et al. 2022

Adv Funct Materials

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With the rapid progress in nanomaterials and biochemistry, there has been an explosion of interest in biomolecule-modified quantum dots (QDs) for biomedical applications. Metal chalcogenide quantum dots (MCQDs), as the most widely studied QDs, have attracted tremendous attention in the biomedical field on account of their unique and excellent optical properties and the ease of biomolecular modifications. Herein, important advances in MCQDs over recent years are reviewed, from materials design to biomedical applications. Especially, this review focuses on the challenges encountered in the applications of MCQDs in biomedical fields and how these problems can be solved by rational design of synthesis methods and modifications, which have opened a universal route to develop the functionalized MCQDs. Moreover, recent processes in bioimaging, biosensing, and cancer therapy based on MCQDs are examined, including the rapid detection and diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This review provides broad insights into MCQDs in the biomedical field and will inspire material researchers to develop MCQDs in the future.

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“…[11][12][13][14] Nowadays, the QD-FRET system has become a powerful tool for the detection of ions, organic small molecules or biological macromolecules. [15][16][17][18][19] Some important studies on the application of FRET in QD-based GSH sensing have also been reported in the last few years. Relevant energy donors for QDs include CdSe/ZnS, 20 MoS 2 , 21 Ag 2 S, 22 Cu: Zn-In-S/ZnS, 23 CdTe, 24 and so on.…”

Section: Introductionmentioning

confidence: 99%

Self-assembled nanoplatforms with ZIF-8 as a framework for FRET-based glutathione sensing in biological samples

Ding

Zhao

et al. 2022

Analyst

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A FRET-based detection of N-acetylneuraminic acid using CdSe/ZnS quantum dot and exonuclease III-assisted recycling amplification strategy

Cited by 12 publications

References 36 publications

Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage

Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage

Recent Advances in Metal Chalcogenide Quantum Dots: From Material Design to Biomedical Applications

Self-assembled nanoplatforms with ZIF-8 as a framework for FRET-based glutathione sensing in biological samples

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