A frequent HindIII RFLP on chromosome 3p21–25 detected by a genomic<i>erbA</i>β sequence

Gareau, J. L. P.; Houle, Benoit; Leduc, François; Bradley, W. E. C.; Dobrovic, Alexander

doi:10.1093/nar/16.3.1223

Cited by 22 publications

(12 citation statements)

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“…2). GAPDH, a "housekeeping enzyme," which is equally expressed in both proliferating and nonproliferating cells (28) (24)(25)(26). Genomic DNA from colon carcinomas and noninvolved colon mucosa were therefore digested separately with Hind III, Msp I, and Dra I, and compared after Southern hybridization to probe pBH302.…”

Section: Resultsmentioning

confidence: 99%

“…Northern probes constructed from human erbA-13 placental clone pheA12 (3) included: probe 1, a near full-length 1.3-kb Eco RI-Bgl II fragment spanning all but the carboxyl terminus of the coding region; probe 2, a 0.3-kb Eco RI-Sst I fragment spanning the NH2 terminus of the coding region; and probe 3, a 0.15-kb Bgl II-Eco RI fragment spanning the COOH terminus of the coding region. An additional erbA-,B probe, pBH302, detects several restriction fragment length polymorphisms (RFLPs)' at the 5' end of the erbA-3 gene (24)(25)(26). The erbA-a probe used was a Bgl I-Hinc II fragment of rat brain clone rbeA 12 (8) spanning all but the carboxyl terminus of the coding region.…”

Section: Methodsmentioning

confidence: 99%

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Expression of the ErbA-beta class of thyroid hormone receptors is selectively lost in human colon carcinoma.

Markowitz

Haut

Stellato³

et al. 1989

J. Clin. Invest.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Expression of the ErbA-beta class of thyroid hormone receptors is selectively lost in human colon carcinoma.

Markowitz

Haut

Stellato³

et al. 1989

J. Clin. Invest.

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“…DNA probes.-The plasmids pH3H2 and pBH302 were maintained in E. coli HB101 and carry, respectively, an anonymous 2-kb HindIII DNA fragment (Carritt et al 1986) and a 2-kb BamHI fragment from the 5' untranslated region of the ERBAP sequence (Gareau et al 1988 figure 1), the only exception being DMS114. This cell line was also informative at DNF1SS2, a locus in band 3p21 at which another HindIII polymorphism exists (Carritt et al 1986).…”

Section: Dna Isolation and Southern Blottingmentioning

confidence: 99%

“…This cell line was also informative at DNF1SS2, a locus in band 3p21 at which another HindIII polymorphism exists (Carritt et al 1986). Blot analysis using the DNF1SS2 probe (Carritt et al 1986) Southern blots of HindlIl-digested DNA from SCLC samples and matching normal tissue probed with BH302 (Gareau et al 1988). DNA was extracted and digested with HindlIl according to the instructions of the supplier, Boehringer-Mannheim.…”

Section: Dna Isolation and Southern Blottingmentioning

confidence: 99%

“…A preliminary survey using densitometric analysis has suggested the loss of at least one copy of this gene in all six SCLC tumors studied . We now report the results of a comprehensive analysis of many lung tumors, using an ERBAOI genomic fragment which detects a HindIII polymorphism for which more than 40% of individuals are constitutionally heterozygous (Gareau et al 1988). …”

Section: Introductionmentioning

confidence: 99%

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Loss of heterozygosity in a gene coding for a thyroid hormone receptor in lung cancers

1989

Lung Cancer

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SummaryThe ERBAB gene codes for a DNA-binding thyroid hormone receptor (THR) and maps to chromosome 3p21-p25, overlapping a 3p deletion characterizing small-cell lung carcinoma (SCLC). A DNA clone detecting an RFLP at the ERBADi locus has been used to probe a large number of lung tumors. Virtually all SCLC had lost heterozygosity, showing that the 3p deletion in SCLC includes this gene. A substantial but smaller proportion of non-small-cell carcinomas had lost heterozygosity at ERBAB. Among all non-smallcell tumors some had lost heterozygosity at the proximal locus DNF15S2 (band 3p2l) but not at ERBADi, whereas none were found where the reverse was true. Therefore, the locus which plays a role in non-smallcell tumorigenesis probably lies closer to DNF15S2 than to ERBAB and is almost certainly not the latter.

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Allelic methylation bias of the RARB2 tumor suppressor gene promoter in cancer

Pappas

Toulouse

Fetni

et al. 2008

Genes Chromosomes & Cancer

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Retinoic acid receptor B2 (RARB2) is frequently inactivated in cancer. Methylation in the 5'-untranslated region and first exon is known to play a role; however, few studies have analyzed the detailed methylation pattern of the promoter region. We show that hypo- and hypermethylated alleles coexist in 5/11 cell lines in which RARB2 is inactivated. We present evidence supporting the mitotic transmission of these divergent methylation patterns and find a correlation between methylation divergence and heterozygosity at the 3p24 loci, suggesting an allelic methylation bias in these lines. Using a newly devised strategy based on allelic identification via methylation-sensitive restriction enzyme digestion combined with the use of a single nucleotide polymorphism, rs755661, we demonstrate that such a bias exists in three cancer cell specimens heterozygous at rs755661 and therefore amenable to this study. This previously unreported phenomenon of allelic methylation bias suggests that a promoter methylation-independent mechanism may be responsible for inactivation at the hypomethylated allele and this inactivation is reminiscent of an aberrant form of de novo imprinting. Approaches to interpreting methylation data should incorporate the notion of allelic methylation bias.

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A frequent HindIII RFLP on chromosome 3p21–25 detected by a genomicerbAβ sequence

Cited by 22 publications

References 1 publication

Expression of the ErbA-beta class of thyroid hormone receptors is selectively lost in human colon carcinoma.

Expression of the ErbA-beta class of thyroid hormone receptors is selectively lost in human colon carcinoma.

Loss of heterozygosity in a gene coding for a thyroid hormone receptor in lung cancers

Allelic methylation bias of the RARB2 tumor suppressor gene promoter in cancer

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