A framework for accurate evaluation of the promise of aquaporin based biomimetic membranes

Grzelakowski, Mariusz; Cherenet, Meron F.; Shen, Yue; Kumar, Manish

doi:10.1016/j.memsci.2015.01.023

Cited by 60 publications

(76 citation statements)

References 37 publications

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“…Stopped flow light scattering measurements to determine vesicle permeability were made using a KinTek SF‐300X stopped flow spectrometer. To increase the signal to noise ratio, only samples with polydispersity 0.3 or less were used (determined from DLS). Because high osmolyte concentrations can lead us to underestimate the membrane permeability (most likely due to concentration polarization), we used dilute ((9–100) × 10 −3 m ) osmolyte solutions for accurate permeability measurements.…”

Section: Methodsmentioning

confidence: 99%

“…The dye we attempt to encapsulate is 5(6) carboxyfluorescein, which is water‐soluble at pH 7 or more; thus, we can test whether the dye is encapsulated in an aqueous vesicle core. Stopped flow light scattering is commonly used to determine membrane permeability and osmolyte rejection when lipid or polymer vesicles are mixed with a hypertonic solution, allowing us to test for the presence and determine the size of pores, or to examine the membrane's intrinsic permeability to solutes . Using these three techniques, we determined that the nanoscale aggregates formed by mixing L121 and F127 are vesicles, and that extrusion significantly reduces their osmotic permeability and molecular weight (MW) cutoff for solute rejection.…”

Section: Introductionmentioning

confidence: 99%

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Porous Vesicles with Extrusion‐Tunable Permeability and Pore Size from Mixed Solutions of PEO–PPO–PEO Triblock Copolymers

Schantz

Ren

Pachalla

et al. 2018

Macro Chemistry & Physics

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Block copolymer (BC) vesicles in aqueous solution can encapsulate hydrophilic molecules or nanoparticles for drug and gene delivery, enhanced imaging, microreactors, or sensors. Inexpensive, biocompatible poly(ethylene oxide)–poly(propylene oxide)–poly(ethylene oxide) (PEO–PPO–PEO) triblock copolymers (TBCs) would be ideal for these applications if they could form concentrated vesicle solutions to encapsulate such molecules with high efficiency. It is shown that solutions of two PEO–PPO–PEO TBCs (EO5–PO68–EO5 and EO100–PO65–EO100) form vesicles, that the vesicle membranes are permeable to low‐molecular weight (MW) solutes, and that extrusion reduces the membrane permeability and MW cutoff. Osmotic permeabilities before and after extrusion are ≈1000 and ≈10 µm s−1, respectively, while the MW cutoffs for solute rejection are ≈1000 and ≈400 g mol−1. Therefore, it is hypothesized that the vesicles contain pores, and that extrusion reduces the pores' size and the membrane's porosity. These selectively permeable vesicles can function as microreactors or sensors encapsulating large solutes without the need to add channel molecules or proteins.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Porous Vesicles with Extrusion‐Tunable Permeability and Pore Size from Mixed Solutions of PEO–PPO–PEO Triblock Copolymers

Schantz

Ren

Pachalla

et al. 2018

Macro Chemistry & Physics

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“…Once the content of incorporated AQPs is augmented, the overall shrinking rate will likewise begin to increase. Nonetheless, the SFLS approach is substantially inluenced by the quality, or size distribution potential, of the polymersomes, the concentration of the osmolytes, and the AQP concentration within the polymersomes [35]. In theory, a visually based measurement can be accomplished using the freeze-fracture transmission electron microscopy (FF-TEM), however, the FF-TEM will not be able to provide suicient data on the functional aspects.…”

Section: Evaluation Of Aqp Incorporation Characterization Methodologiesmentioning

confidence: 99%

Interactions between Aquaporin Proteins and Block Copolymer Matrixes

Abdelrasoul¹,

Doan²,

Lohi³

2017

Biomimetic and Bioinspired Membranes for New Frontiers in Sustainable Water Treatment Technology

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This chapter continues to further expand its focus on aquaporins (AQPs) by ofering a general outline on how the AQPs block copolymers, and polymer support structures can interrelate and such connections can be comprehensively classiied and deined. The irst section of the overview will consider the relationship between block copolymers and AQPs. It will also examine the general membrane protein integration into block copolymers, since this can cause AQP-block copolymer complexes in vesicular (proteopolymersomes) as well as in planar forms. The majority of considerations taken into account during AQP incorporation come from the research conducted in relation to the process of incorporating other types of membrane proteins. This chapter includes an overview of the various characterization methodologies needed for the study of proteopolymersomes, as well as freeze-fracture transmission electron microscopy (FF-TEM), luorescence correlation spectroscopy (FCS), small-angle X-ray scatering (SAXS), and stopped-low light scatering (SFLS). The research data presented in this chapter emphasizes the fact that a successful process of membrane fabrication requires the integration of reconstituted AQPs into a suitable supporting matrix formation.Keywords: aquaporin proteins, block copolymer, matrix, vesicular, membrane protein Assessing aquaporin proteins and block copolymer matrixes interactionsMost research performed on membrane protein inclusion has been conducted primarily with lipids as the host matrix components (original proteoliposomes publication on the subject came out in 1971) [1]. Since then, polymer-based incorporation process has received increased atention and the earliest proteopolymersomes publication emerged in 2000 [2]. The initial work stages in this research area concentrated on the inclusion of membranespanning proteins, such as membrane-bound ion channels (ATPases) and bacteriorhodopsin incorporation into polymethyloxazoline-polydimethylsiloxane-polymethyloxazoline (PMOXA-PDMS-PMOXA) triblock copolymer bilayers occurring in planar [3] or vesicular forms [4][5][6]. It is quite fascinating that the membrane proteins may be functionally incorporated into polymeric bilayers (e.g., based on PMOXA-PDMS-PMOXA) that occur up to 10 times thicker than their lipidic counterparts [7]. Moreover, researchers have observed proteopolymersomes with protein density values that drastically surpassed those of proteoliposomes [8]. Research on these phenomena has helped to establish a theoretical approach for generalized membrane protein incorporation into the amphiphilic structures. This methodology is based on the notion that the eiciency of the membrane protein incorporation process relies on its hydrophobicity potential and its coupling capacity to the host membrane is directly connected to the hydrophobic mismatch parameters. In order to reduce this mismatch dynamic, the method calls for the host membrane to be deformed in such a way that it matches the hydrophobic length parameter of the membrane protein's transm...

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“…Assuming only one SLB layer was formed on the support and all AqpZ were incorporated in the SLB, the water permeability of AqpZ in the biomimetic membrane (P AqpZ-SLB ) was calculated according to the following equation: 26 …”

Section: Fo and Nf Performancesmentioning

confidence: 99%

Fabrication of an aquaporin-based forward osmosis membrane through covalent bonding of a lipid bilayer to a microporous support

et al. 2015

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AquaporinZ (AqpZ)-containing, planar, biomimetic membranes hold great application potential in water purification and seawater desalination, due to the excellent permeability and selectivity of AqpZ. However, there remain many challenges to production of robust and defect-free supported lipid bilayer (SLB) biomimetic membranes. By forming amide bonds between the lipid bilayer and microporous substrate, we fabricated an AqpZ-incorporated SLB forward osmosis (FO) membrane, with a large area of 36 cm 2 . With deionized water and 2 mol/L MgCl 2 draw solution, the AqpZ-incorporated biomimetic membrane exhibited a water flux of ~19.2 L·m −2 ·h −1 (LMH) and a reverse solute flux of ~3.2 g·m −2 ·h −1 (gMH). When positively charged phospholipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) was blended in the 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) bilayer, a higher flux (~23.1 LMH) could be reached accompanied with a constant reverse salt flux of 3.1 gMH, due to more AqpZ being embedded in the mixed bilayer. From nanofiltration (NF) test, the water permeability (A) could reach 6.31 LMH/bar with a relative low solute permeability (B) of 1.7 LMH for AqpZ-DOPE/DOTAP SLB membrane. When rinsed by a 0.24 mmol/L TritonX-100 (TX-100) surfactant solution, water flux and reverse salt flux of the biomimetic membrane with covalent bond only slightly increased, whereas the membrane without covalent bonds showed significant increase in both water flux and reverse salt flux after TX-100 treatment.This paper presented an effective method for preparation of biomimetic FO membrane with good separation performance as well as excellent stability and durability. Graphical Abstract IntroductionIn recent decades, forward osmosis (FO) has gradually emerged as one of the most promising water purification and desalination technologies in water supply, energy generation, and food processing. 1-4 Unlike pressure-driven membrane processes, like reverse osmosis (RO) and nanofiltration (NF), FO is a naturallyoccurring osmosis-driven membrane process that takes advantage of the osmotic pressure gradient to impel water across a semipermeable membrane, from the feed solution side with high chemical potential,

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A framework for accurate evaluation of the promise of aquaporin based biomimetic membranes

Cited by 60 publications

References 37 publications

Porous Vesicles with Extrusion‐Tunable Permeability and Pore Size from Mixed Solutions of PEO–PPO–PEO Triblock Copolymers

Porous Vesicles with Extrusion‐Tunable Permeability and Pore Size from Mixed Solutions of PEO–PPO–PEO Triblock Copolymers

Interactions between Aquaporin Proteins and Block Copolymer Matrixes

Fabrication of an aquaporin-based forward osmosis membrane through covalent bonding of a lipid bilayer to a microporous support

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