A fragmentation-based method for the differentiation of glutathione conjugates by high-resolution mass spectrometry with electrospray ionization

Identification and characterization of reactive metabolites in myristicin-mediated mechanism-based inhibition of CYP1A2

“…(Fig. 4C), which is useful fragment information for metabolite identification and is also in agreement with previous reports [31]. The structural information of all of the representative fragment ions in this GSH-M1 adduct has been analyzed completely, and the detailed cleavage process of the GSH-M1 adduct for MS 2 is shown in Fig.…”

“…GSH acted as a trapping agent to identify the structure of RMs [29], and this classical approach is widely employed in the detection of RMs [30]. A comprehensive understanding of the fragmentation behavior of GSH [31] was helpful for analyzing the behavior of GSH-myristicin adducts. In this work, compared with the control group, incubating GSH with myristicin in HLMs resulted in one obvious peak of myristicin at 5.10 min in a positive-mode chromatogram (Fig.…”

Identification of glutathione adducts of α-chlorofatty aldehydes produced in activated neutrophils

“…To confi rm that ionization and fragmentation patterns of the deuterated product were comparable to the natural product during this LC/MS/ MS analysis, natural HDA-GSH levels were varied while ( Fig. 3B ) that directly correspond to the known fragmentation of GSH ( 29,30 ). The predominating ion, m/z 272.16, is the cleavage of HDA-GSH at the sulfhydryl as shown in the Fig.…”

Current trends in environmental analysis of human metabolite conjugates of pharmaceuticals

“…Using this phase, various antimicrobial compounds including triclosan were analyzed in rat plasma [37] and hoseradish extract [38]. Also analyzed were resveratrol [39] and 52 glutathione conjugates of various pharmaceuticals which were prepared by microbial incubation of the parent compounds [40]. The main advantage of the HSS T3 column was to maximise separation due to the stationary phase's resistance to degradation through extensive end-capping.…”