A fragment of theneurogenin1 gene confers regulated expression of a reporter gene in vitro and in vivo

Murray, Richard C.; Tapscott, Stephen J.; Petersen, Jean W.; Calof, Anne L.; McCormick, Mary

doi:10.1002/(sici)1097-0177(200005)218:1<189::aid-dvdy16>3.0.co;2-4

Cited by 15 publications

(12 citation statements)

References 26 publications

(61 reference statements)

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“…1, 2C,C′). This is consistent with early studies that showed this region worked only inefficiently at directing expression of a reporter to Neurog1 domains in transgenic mice (Murray et al, 2000; Nakada et al, 2004). …”

Section: Resultssupporting

confidence: 91%

“…As such, the temporal and spatial control of Neurog1 expression is complex and includes multiple discrete regulatory regions spread over more than 20 kb surrounding the coding exon. Over the past decade, work in zebrafish and mouse has identified multiple cis-regulatory sequences for Neurog1 using sufficiency assays in transgenic animals (Blader et al, 2004; Blader et al, 2003; Gowan et al, 2001; Murray et al, 2000; Nakada et al, 2004). Here, using BAC transgenic mice to test the requirement of these sequences in directing neural specific expression in vivo, we find additional complexities including an apparent redundancy in enhancer activity, new activities for a previously defined enhancer, and localization of an enhancer for ventral neural tube expression.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies in mouse that tested regions of Neurog1 flanking sequence across a 15 kb region identified multiple intergenic regions sufficient to direct expression of reporter genes to a subset of the Neurog1 expression domain (Blader et al, 2004; Gowan et al, 2001; Murray et al, 2000; Nakada et al, 2004). However, these regulatory regions were not sufficient to recapitulate the entire Neurog1 pattern.…”

Section: Introductionmentioning

confidence: 99%

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Neurogenin 1 (Neurog1) expression in the ventral neural tube is mediated by a distinct enhancer and preferentially marks ventral interneuron lineages

Quiñones

Savage

Battiste

et al. 2010

Developmental Biology

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The bHLH transcription factor Neurog1 (Ngn1, Neurod3, neurogenin 1) is involved in neuronal differentiation and cell-type specification in distinct regions of the developing nervous system. Here, transgenic mouse models were developed that use a Bacterial Artificial Chromosome (BAC) containing 208 kb flanking the Neurog1 gene to efficiently drive expression of GFP and Cre in all Neurog1 domains. Two characteristics of Neurog1 gene regulation were uncovered. First, a 4 kb region previously shown to be sufficient for driving expression of a reporter gene to a subset of the Neurog1 pattern in the developing midbrain, hindbrain, and spinal cord is required uniformly for high levels of expression in all Neurog1 domains, even those not originally identified as being regulated by this region. Second, a 0.8 kb enhancer was identified that is sufficient to drive Neurog1-like expression specifically in the ventral neural tube. Furthermore, Neurog1 progenitor cells in the ventral neural tube are largely fated to interneuron lineages rather than to motoneurons. These studies provide new tools for directing tissue specific expression in the developing neural tube, define Neurog1 lineages in the spinal cord, and further define the complex genomic structure required for obtaining the correct levels and spatial restriction of the neuronal differentiation gene Neurog1.

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Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Neurogenin 1 (Neurog1) expression in the ventral neural tube is mediated by a distinct enhancer and preferentially marks ventral interneuron lineages

Quiñones

Savage

Battiste

et al. 2010

Developmental Biology

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“…We found a consensus sequence for TCF binding (van de Wetering et al, 1997) located at nucleotide (nt) positions -1167 to -1160 relative to the transcription start site of the mouse Ngn1 gene. This region within the promoter has been shown to be responsible for expression of the gene in the dorsal neocortex during neurogenesis (Murray et al, 2000). To determine whether this TCF binding element is functional, we compared the activities of the Ngn1 gene promoter (nt -2670 to +74) containing either an intact or mutated version of this DNA sequence (Fig.…”

Section: Direct Regulation Of the Ngn1 Promoter By The β-Catenin/tcf mentioning

confidence: 99%

The Wnt/β-catenin pathway directs neuronal differentiation of cortical neural precursor cells

et al. 2004

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vitro and in the developing mouse neocortex. Furthermore, the β-catenin/TCF complex appears to directly regulate the promoter of neurogenin 1, a gene implicated in cortical neuronal differentiation. Importantly, stabilized β-catenin did not induce neuronal differentiation of cortical NPCs at earlier developmental stages, consistent with previous reports indicating self-renewal-promoting functions of Wnts in early NPCs. These findings may reveal broader and stage-specific physiological roles of Wnt signaling during neural development.

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“…These residues are indicated on the corresponding model (right part of figure). around 140 nucleotides upstream of the start of the transcription site of neurogenin1, a transcription factor essential for neuronal differentiation and subtype specification during embryogenesis (33,34). Interestingly, when NZF3, a member of the same protein family to which MyT1 belongs, and Neurog1 are co-expressed in cells, the rate of neuronal differentiation is significantly increased, suggesting that these proteins act in concert to stimulate these processes (35).…”

Section: Differences In the Dna Binding Mode Between Myt1mentioning

confidence: 99%

A Structural Analysis of DNA Binding by Myelin Transcription Factor 1 Double Zinc Fingers

Gamsjaeger

O’Connell

Cubeddu

et al. 2013

Journal of Biological Chemistry

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Background: Myelin transcription factor 1 (MyT1) contains seven similar zinc finger domains that bind DNA specifically. Results: A three-dimensional structural model explains how a double zinc finger unit is able to recognize DNA. Conclusion: DNA-binding residues are conserved among all MyT1 zinc fingers, suggesting an identical DNA binding mode. Significance: Determination of the molecular details of DNA interaction will be crucial in understanding MyT1 function.

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A fragment of theneurogenin1 gene confers regulated expression of a reporter gene in vitro and in vivo

Cited by 15 publications

References 26 publications

Neurogenin 1 (Neurog1) expression in the ventral neural tube is mediated by a distinct enhancer and preferentially marks ventral interneuron lineages

Neurogenin 1 (Neurog1) expression in the ventral neural tube is mediated by a distinct enhancer and preferentially marks ventral interneuron lineages

The Wnt/β-catenin pathway directs neuronal differentiation of cortical neural precursor cells

A Structural Analysis of DNA Binding by Myelin Transcription Factor 1 Double Zinc Fingers

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