A four-allele RFLP identified by an anaonymous single copy genomic clone at 13q21-13qter [HGM8 assignment no. D13S12]

Scheffer, Hans; Penninga, Dirk; Goor, Nicole; Pearson, P. L.; Buys, Charles H.C.M.

doi:10.1093/nar/14.10.4374

Cited by 6 publications

(2 citation statements)

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“…All other samples including several controls (lym phocyte DNA) displayed the regular pattern of av erage intensity. As a control of the amount of DNA loaded in each lane, all filters were washed and rchybridized with a single copy probe derived from chro mosome 13 and yielding a typical 2.4 EcoRI fragment [14]. All lanes were shown to contain comparable amounts of DNA ( fig.…”

Section: Resultsmentioning

confidence: 99%

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Gene Amplification and Expression of the <i>neu </i>(c-erbB-2) Sequence in Human Mammary Carcinoma

Fontaine

Tesseraux²,

Klein³

et al. 1988

Oncology

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Alterations in the structure and expression of the neu oncogene were investigated in 15 human mammary tumors. In 7 of the samples (46%), amplification by the factor up to 14 was detected, some samples displaying an additional EcoRI restriction fragment at 3.4 kb. In tumor tissue with amplified neu sequences from which intact RNA was available, enhanced expression was noted. Correlation of the molecular data to clinical parameters indicated a correlation of the neu oncogene amplification to tumor grading and thus poor prognosis.

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Section: Resultsmentioning

confidence: 99%

“…The neu oncogene recombinant (pneuct) was provided by R. Weinberg, Cambridge. Mass., USA [12], The controls were performed using a P-actin [13] and a chro mosome 13-specific probe (D13S12) [14], Quantitation of signals was obtained from densitométrie scanning of the autoradiograms (LKB Ultroscan).…”

Section: Tissuementioning

confidence: 99%

Gene Amplification and Expression of the <i>neu </i>(c-erbB-2) Sequence in Human Mammary Carcinoma

Fontaine

Tesseraux²,

Klein³

et al. 1988

Oncology

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Close linkage of the Wilsons's disease locus to D13S12 in the chromosomal region 13q21 and not to ESD in 13q14

et al. 1990

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Wilson's disease (WD) is an autosomal recessive disorder resulting in copper accumulation notably in liver and brain tissue. Linkage of the WD locus (WND) to ESD at 13q14 was first shown by studies in families of Middle Eastern origin using the isozymic polymorphism of esterase D. Using RFLPs detected by the ESD cDNA we could not confirm this reported close linkage in an analysis of 17 WD families of northwest European origin. A tight linkage was detected, however, to the marker D13S12, located more distally at 13q21. No obligate cross-overs were detected in 63 gametes informative for this marker. Our data confirm an assignment of WND to 13q14-21. Its localization, however, seems to be more distal to ESD than previously reported. Although genetic heterogeneity cannot be excluded, the observed differences between the two populations are probably due to random variation.

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Identification of crossovers in Wilson disease families as reference points for a genetic localization of the gene

et al. 1992

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Wilson disease (WD) is an autosomal recessive disorder of copper metabolism. A minimum recombinant analysis using D13S22, ESD, RB1, D13S31, D13S55, D13S26, D13S39, and D13S12, all localized at 13q14-q22, has been carried out in 20 WD families of Northwest-European origin. No inconsistencies have been observed with respect to locus order or location of the WD locus (WND) compared with previous linkage studies. D13S31 was mapped as the closest marker proximal to WND, whereas D13S55 and D13S26 were mapped as the closest markers distal to WND. We have identified a crossover between WND and D13S31 in one family and a crossover between WND and D13S55 in another. These crossover sites can be used as reference points for new chromosome 13q14-q21 markers, and are therefore important for a more accurate mapping of the WD locus.

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A four-allele RFLP identified by an anaonymous single copy genomic clone at 13q21-13qter [HGM8 assignment no. D13S12]

Cited by 6 publications

References 0 publications

Gene Amplification and Expression of the <i>neu </i>(c-erbB-2) Sequence in Human Mammary Carcinoma

Gene Amplification and Expression of the <i>neu </i>(c-erbB-2) Sequence in Human Mammary Carcinoma

Close linkage of the Wilsons's disease locus to D13S12 in the chromosomal region 13q21 and not to ESD in 13q14

Identification of crossovers in Wilson disease families as reference points for a genetic localization of the gene

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