A Formiminotransferase Cyclodeaminase Isoform Is Localized to the Golgi Complex and Can Mediate Interaction of Trans-Golgi Network-derived Vesicles with Microtubules

Hennig, Dagmar; Scales, Suzie J.; Moreau, Anne; Murley, Laura Lea; Mey, J. De; Kreis, Thomas E.

doi:10.1074/jbc.273.31.19602

Cited by 40 publications

(39 citation statements)

References 54 publications

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“…Although the hypothesis that 58K (FTCD) links Golgi membranes to MTs in vivo must now be regarded with skepticism, an unexpected association of FTCD with the Golgi apparatus has been revealed. It is noteworthy that analogous results were obtained independently in another laboratory for a chicken liver version of FTCD, and are reported in Henning et al (2).…”

supporting

confidence: 70%

“…Curiously, 58K was localized by immunofluorescence to the Golgi apparatus in cultured hepatoma cells and was present on purified liver Golgi membranes as a peripheral membrane protein (1). 58K was also detected by both Western blotting 2 and immunofluorescence microscopy (18,19) in a wide variety of cultured mammalian cell types. As was found for hepatoma cells, immunofluorescence labeling of other cell types with anti-58K antibodies yielded bright staining of the Golgi complex.…”

mentioning

confidence: 99%

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58K, a Microtubule-binding Golgi Protein, Is a Formiminotransferase Cyclodeaminase

Bashour¹,

Bloom

1998

Journal of Biological Chemistry

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supporting

confidence: 70%

mentioning

confidence: 99%

58K, a Microtubule-binding Golgi Protein, Is a Formiminotransferase Cyclodeaminase

Bashour¹,

Bloom

1998

Journal of Biological Chemistry

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“…p115 is a peripherally associated Golgi protein that is found in lesser amounts in the ER (Waters et al, 1992). FTCD is a 58 kDa Golgi-associated protein that can bind to MTs and IFs (Bashour and Bloom, 1998;Gao et al, 1998;Hennig et al, 1998). MACF1b was found to be present chiefly in the Golgi-and not the ERenriched fractions, suggesting that MACF1b associated with the Golgi complex in H460 cells (Fig.…”

Section: Macf1b Localizes To the Golgi Complexmentioning

confidence: 99%

“…Hook 3 participates in defining the structure and localization of the Golgi complex (Walenta et al, 2001). Association of IFs with the Golgi complex mediated through FTCD has been described (Bashour and Bloom, 1998;Gao et al, 1998;Hennig et al, 1998). FTCD was originally identified as a novel MT-binding protein that can associate with both the Golgi complex and MTs (Bloom and Brashear, 1989), but was later found to bind vimentin and stimulate vimentin filaments formation.…”

Section: Genomic Organization Of Macf1mentioning

confidence: 99%

Microtubule actin crosslinking factor 1b: a novel plakin that localizes to the Golgi complex

Lin

Chen

Leung

et al. 2005

Journal of Cell Science

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MACF1 (microtubule actin crosslinking factor), also called ACF7 (actin crosslinking family 7) is a cytoskeletal linker protein that can associate with both actin filaments and microtubules. We have identified a novel alternatively spliced isoform of MACF1. We named this isoform MACF1b and renamed the original isoform MACF1a. MACF1b is identical to MACF1a, except that it has a region containing plakin (or plectin) repeats in the middle of the molecule. MACF1b is ubiquitously expressed in adult tissues with especially high levels in the lung. We studied the subcellular localization of MACF1b proteins in mammalian cell lines. In two lung cell lines, MACF1b was chiefly localized to the Golgi complex. Upon treatments that disrupt the Golgi complex, MACF1b redistributed into the cytosol, but remained co-localized with the dispersed Golgi ministacks. MACF1b proteins can be detected in the enriched Golgi fraction by western blotting. The domain of MACF1b that targets it to the Golgi was found at the N-terminal part of the region that contains the plakin repeats. Reducing the level of MACF1 proteins by small-interfering RNA resulted in the dispersal of the Golgi complex.

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“…8 FTCD is a soluble cytosolic protein, but in cells appears to be preferentially associated with the cytosolic side of Golgi membranes. [9][10][11] In addition, FTCD appears to be a dynamic component of the Golgi, and cycles between the Golgi and earlier compartments of secretory pathways. 9 In this report, we show that a human liver cytosol protein immunoprecipitated with a pool of LC1-positive autoimmune sera is FTCD, and that LC1 sera recognize distinct epitopes on FTCD.…”

mentioning

confidence: 99%

Distinct epitopes on formiminotransferase cyclodeaminase induce autoimmune liver cytosol antibody type 1

Muratori

2001

Hepatology

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Liver cytosol antibody type 1 (LC1) is regarded as a serologic marker of type 2 autoimmune hepatitis, in addition to liver kidney microsomal antibody type 1. Among 38 patients with type 2 autoimmune hepatitis, 23 were positive for LC1 antibodies. The antigen recognized by LC1 has been identified as a liver-specific 58-kd metabolic enzyme named formiminotransferase cyclodeaminase (FTCD). All 23 LC1-positive sera immunoprecipitated rat FTCD, and 22 gave an identity reaction with rat FTCD by immunodiffusion. No reaction was observed with sera from 10 patients with type 1 autoimmune hepatitis, 10 with primary biliary cirrhosis, 10 with chronic hepatitis C, and 10 healthy controls. By Western immunoblotting all 23 LC1-positive sera and all the controls tested negative, suggesting that all the antigenic epitopes were destroyed by denaturation. Liver cytosol antibody type 1 (LC1) has been first described, alone or in association with liver kidney microsomal antibody type 1 (LKM1), in patients with type 2 autoimmune hepatitis. 1,2 Only sporadically is it detected in patients with chronic hepatitis C virus infection, usually in association with LKM1. 3,4 Among the different autoantibodies deemed as serologic markers of autoimmune hepatitis, 5 LC1 is particularly intriguing because it targets a liver-specific antigen. 1,2 In addition, serum LC1 concentrations appear to fluctuate in parallel with aminotransferase levels, an observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanisms leading to hepatocyte injury. 6 In a recent report, Lapierre et al. after screening a complementary DNA (cDNA) library of HepG2 cells with an LC1-positive serum isolated a clone with high sequence homology to pig formiminotransferase cyclodeaminase (FTCD), and showed that a construct encoding the C-terminal portion of FTCD can be recognized by LC1 antibodies. 7 FTCD is a mammalian metabolic enzyme involved in the conversion of histidine to glutamic acid, 8 and is most highly expressed in the liver. FTCD is bifunctional and is composed of distinct FT and CD domains connected by a short linker. The FT activity transfers a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the CD activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. Native FTCD is an octamer with 8 identical subunits arranged in a planar ring. 8 FTCD is a soluble cytosolic protein, but in cells appears to be preferentially associated with the cytosolic side of Golgi membranes. [9][10][11] In addition, FTCD appears to be a dynamic component of the Golgi, and cycles between the Golgi and earlier compartments of secretory pathways. 9 In this report, we show that a human liver cytosol protein immunoprecipitated with a pool of LC1-positive autoimmune sera is FTCD, and that LC1 sera recognize distinct epitopes on FTCD. We used full-length recombinant rat FTCD as antigenic substratum for immunodiffusion, immunoblotting, and immu...

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A Formiminotransferase Cyclodeaminase Isoform Is Localized to the Golgi Complex and Can Mediate Interaction of Trans-Golgi Network-derived Vesicles with Microtubules

Cited by 40 publications

References 54 publications

58K, a Microtubule-binding Golgi Protein, Is a Formiminotransferase Cyclodeaminase

58K, a Microtubule-binding Golgi Protein, Is a Formiminotransferase Cyclodeaminase

Microtubule actin crosslinking factor 1b: a novel plakin that localizes to the Golgi complex

Distinct epitopes on formiminotransferase cyclodeaminase induce autoimmune liver cytosol antibody type 1

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