A food ingredient containing phytoecdysteroids and polyphenols from quinoa grain: technology and physiological and biochemical evaluation in vivo

Петров, Н. А.; Biryulina, Nadezhda; Sidorova, Yuliya S.; Мазо, В К

doi:10.1051/e3sconf/202128505014

Cited by 2 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each experiment was repeated three times to obtain an average. Relative conductivity was measured using the method of Petrov et al [12]. The proline content was determined using the sulfosalicylic acid method, the MDA content by the thiobarbituric acid-spectrophotometric method, and POD activity using the guaiacol method [13].…”

Section: Determination Of Physiological and Biochemical Indicatorsmentioning

confidence: 99%

Development of a New Cold-Tolerant Maize (Zea mays L.) Germplasm Using the ICE1 Gene from Arabidopsis thaliana

Qu¹,

Liu²,

Jiao³

et al. 2022

Phyton

View full text Add to dashboard Cite

To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION (ICE1) expression in response to cold stress, RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar. Immature maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated transformations. From the regenerated plantlets, three T1 lines were screened and identified by PCR. A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize (Zea mays L.) genomes of the three T1 generations. Under low temperature-stress conditions (4°C), the relative conductivity levels decreased by 27.51%-31.44%, the proline concentrations increased by 12.50%-17.50%, the malondialdehyde concentrations decreased by 16.78%-18.37%, and the peroxidase activities increased by 19.60%-22.89% in the T1 lines compared with those of the control. A real-time quantitative PCR analysis showed that the ICE1 gene was ectopically expressed in the roots, stems, and leaves of the T1 lines. ICE1 positively regulates the expression of the CBF genes in response to cold stress. Thus, this study showed the successful transformation of maize with the ICE1 gene, resulting in the generation of a new maize germplasm that had increased tolerance to cold stress.

show abstract

Section: Determination Of Physiological and Biochemical Indicatorsmentioning

confidence: 99%

Development of a New Cold-Tolerant Maize (Zea mays L.) Germplasm Using the ICE1 Gene from Arabidopsis thaliana

Qu¹,

Liu²,

Jiao³

et al. 2022

Phyton

View full text Add to dashboard Cite

show abstract

Physiological and Biochemical Evaluation of the Effectiveness of a New Food Ingredient, a Source of Phytoecdysteroids and Flavonoids from Quinoa Grain

Петров

Зорин

Biryulina

et al. 2021

Biiotehnologiâ (Mosk.)

View full text Add to dashboard Cite

Abstract- One of the promising food sources of biologically active substances is quinoa grain, which is valued for its high content of protein, sulfur-containing amino acids, lysine, fiber, and minerals. In addition, quinoa grain can be a valuable food source of polyphenolic compounds and phytoecdysteroids. The method for production of a concentrate of flavonoids and 20-hydroxyecdysone from quinoa grains sorbed on coagulated egg protein has been developed. The in vivo evaluation of efficacy of the developed food ingredient was conducted using male Wistar rats under immobilization stress and after exhausting physical exertion. The consumption of the food ingredient prevented an increase in the level of the main stress markers, catecholamines, in animals subjected to immobilization stress. The opposite effect was observed in animals that received the food ingredient after exhausting physical exertion: their levels of catecholamines were significantly higher than in the rest comparison groups. Using the Elevated Plus Maze and Open Field tests, it was shown that the consumption of the developed concentrate neutralized the negative effect of immobilization stress and treadmill exercise on anxiety in Wistar rats. The results obtained require additional study under conditions of preventive introduction of the food ingredient into the diet of intact animals, as well as a toxicological safety assessment. Key words: quinoa, 20-hydroxyecdysone, flavonoids, stress, immobilization, exhaustive physical exercise, catecholamines, corticosterone This work was supported by the Russian Scientific Foundation, grant no. 19-16-00107.

show abstract

A food ingredient containing phytoecdysteroids and polyphenols from quinoa grain: technology and physiological and biochemical evaluation in vivo

Cited by 2 publications

References 11 publications

Development of a New Cold-Tolerant Maize (Zea mays L.) Germplasm Using the ICE1 Gene from Arabidopsis thaliana

Development of a New Cold-Tolerant Maize (Zea mays L.) Germplasm Using the ICE1 Gene from Arabidopsis thaliana

Physiological and Biochemical Evaluation of the Effectiveness of a New Food Ingredient, a Source of Phytoecdysteroids and Flavonoids from Quinoa Grain

Contact Info

Product

Resources

About