A focus on the discovery of potent and selective cyclic peptide scaffolds for drug development

Abstract

Library-based screening methods such as mRNA display are paving the way for the discovery of cyclic peptides towards previously undruggable space.

Identification and engineering of potent cyclic peptides with selective or promiscuous binding through biochemical profiling and bioinformatic data analysis

“…Modern display technologies such as phage, ribosome or mRNA display enable the rapid identification of potent and selective CP ligands, for subsequent use in biological experiments or drug discovery efforts. [1][2][3] In a previous study, we demonstrated the use of an efficient, high throughput strategy from next generation sequencing (NGS) hits to identifying high affinity CP binders. 4 In this study, we aimed to investigate different avenues to develop CP binders against a protein family, with differing target binding profiles which either interact with a target selectively or provide a specific interaction profile (binding to only a subset of targets).…”