A focus on high‐content cytometry

Automated quantification of DNA demethylation effects in cells via 3D mapping of nuclear signatures and population homogeneity assessment

“…Topological analysis of the distribution of proteinaceous and nucleic acid components of the cell, in particular mammalian cell nuclei, is helpful in understanding cellular functions in the state of health versus disease (1–10). Correlations between the distribution of cellular proteins and/or fractions of nuclear DNA and certain diseases has allowed mammalian cells to be utilized as useful models in the search for appropriate disease treatment, in the context of systems biology (11, 12). With the availability of today's more advanced imaging approaches (including confocal laser scanning microscopy, two‐photon excitation microscopy, high content cell imaging, and automated tissue scanning), high resolution optical imaging has evolved into an essential tool for moving new chemical entities through the pharmaceutical discovery pipeline utilizing cell‐based assays.…”

Going into lengths and widths, and depths—Microscopic cytomics quantifying cell function and cell communication

“…This background raises the hope that these assays will make their way to clinical applications. This development gets more and more visible for the last two years; during that time several special issues of Cytometry Part A have focused on high‐content cytometry and systems biology (22, 23) to which this issue serves as an update.…”

Patch bandits

“…Multiplexing quantitative single cell analysis is a continuing issue in cytometry and was the focus of numerous recent publications (13,14) using quantitative microscopy of flow cytometry technologies. Recently, Paar et al (15) combined FRET with total internal reflectance microscopy (TIRFM) for high throughput screening.…”