A fluorospectrophotometric study on the binding of acridine orange with DNA and its bases

Yamabe, Shigeru

doi:10.1016/0003-9861(69)90020-4

Cited by 29 publications

(4 citation statements)

References 20 publications

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“…It is interesting to note that depurination greatly reduces the number of such interactions. This observation is consistent with previous reports which indicate that acridine dyes form complexes with purine-containing nucleic acid derivatives which are stronger and lead to more dramatic spectral changes than complexes with pyrimidine-containing derivatives (Peacocke and Skerrett, 1956;Hruska and Danyluk, 1968;Yamabe, 1969).…”

Section: Fluorescence Colorsupporting

confidence: 93%

The Interaction of Acridine Dyes with the Densely Packed DNA of Bacteriophage

Dusenbery¹,

Uretz²

1972

Biophysical Journal

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The interactions of acridine dyes with intact phage DNA differ from those with extracted DNA in the following respects. Strong binding (intercalation) is greatly reduced in intact phage but probably not eliminated. The cooperative, weak binding is stronger and the stacking tendency is increased. In gels of DNA the stacking tendency is seen to increase with decreasing hydration. These influences of the dense packing of DNA must be taken into account when using basic dyes to study chromosome structure.

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Section: Fluorescence Colorsupporting

confidence: 93%

The Interaction of Acridine Dyes with the Densely Packed DNA of Bacteriophage

Dusenbery¹,

Uretz²

1972

Biophysical Journal

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“…Before freezing Extinction Area (UA)* (Ww* staining or Feulgen-DNA cytophotometric study. Acridine-orange staining is a wellknown method for studying the cytochemical characterization of desoxyribonucleoproteins and DNA [Rigler et al, 1969;Yamabe, 1969;Ichimura, 19751. Green fluorescence indicates intercalated A 0 monomers between parallel bases in an expanded double-stranded DNA helix.…”

Section: Table 1 Freezing-thawing Effects On Feulgen-dna Content Andmentioning

confidence: 99%

Freezing and thawing alter chromatin stability of ejaculated human spermatozoa: Fluorescence acridine orange staining and Feulgen‐DNA cytophotometric studies

et al. 1988

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Cryopreservation and freezing-thawing effects on the fertilizing ability of human spermatozoa commonly are evaluated by post-thaw motility. Various studies have depicted the ultrastructural changes caused by freezing-thawing, yet the chromatin alterations have been studied very limitedly. Our aim was to determine the extent to which freezing-thawing alters the chromatin of human spermatozoa, using two analytical methods: acridine orange staining and Feulgen-DNA cytophotometric studies. Both methods revealed a dramatic effect of freezing-thawing on sperm chromatin: the native DNA content decreased as did the Feulgen-DNA content, and sperm surface area was reduced. These results indicate an effect on DNA, diminished accessibility for Feulgen, and a decrease in nuclear surface area and prompt us to hypothesize a relationship between an "overcondensation" state for sperm chromatin after freezing-thawing and a lower conception rate for human semen after cryostorage.

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“…Subsequently, it became conventional to consider aminoacridine/DNA complexes of low dye/DNA ratios to be intercalation complexes (3). On the basis of optical studies, intercalation binding of acridine orange has been discussed or inferred by several authors (34,39).…”

Section: Resultsmentioning

confidence: 99%

Changes in Superhelical Density of Closed Circular Deoxyribonucleic Acid by Intercalation of Anti-R-Plasmid Drugs and Primaquine

Allison

Hahn

1977

Antimicrob Agents Chemother

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The following compounds, which possess anti-R-plasmid activity (Hahn and Ciak, 1976) in decreasing order, were shown by viscometric titration to change systematically the superhelical density of closed circular PM2 deoxyribonucleic acid in the manner of intercalators: ethidium bromide, quinacrine, acridine orange, quinine, chlorpromazine, chloroquine, and methylene blue. The same effect was caused by the antimalarial drug primaquine, which has not been tested for antiplasmid activity.

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A fluorospectrophotometric study on the binding of acridine orange with DNA and its bases

Cited by 29 publications

References 20 publications

The Interaction of Acridine Dyes with the Densely Packed DNA of Bacteriophage

The Interaction of Acridine Dyes with the Densely Packed DNA of Bacteriophage

Freezing and thawing alter chromatin stability of ejaculated human spermatozoa: Fluorescence acridine orange staining and Feulgen‐DNA cytophotometric studies

Changes in Superhelical Density of Closed Circular Deoxyribonucleic Acid by Intercalation of Anti-R-Plasmid Drugs and Primaquine

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