A fluorometric assay for quantification of fucoidan, a sulfated polysaccharide from brown algae

Abstract

Brown algae contain a fucose-rich sulfated polysaccharide called fucoidan which is a constituent of the extracellular matrix. In recent years, fucoidan has attracted much attention for its pharmaceutically important biological activities. To develop a superior method for fucoidan quantification, we investigated several cationic dyes with potential interaction with the negatively charged sulfate groups of fucoidan and found that a commercially available fluorescent dye, SYBR Gold nucleic acid gel stain, showed a clear fluorescence enhancement upon mixing with authentic fucoidan. A standard curve generated with the dye and authentic fucoidan showed a linear dynamic range of 0.05-2.5 ng µl −1 , and the limits of detection and quantification of fucoidan were 0.025 and 0.075 ng µl −1 , respectively. Neither nucleic acids nor alginates, anionic polymers present in brown algae, interfered with the quantification of fucoidan, which is simply extracted with diluted HCl from sporophytes of the brown alga Saccharina japonica. The fluorometric microplate method in this study is simple, sensitive, and widely applicable to fucoidan quantification.

Stimulation of the Biofilms of a marine bacterium Pseudoalteromonas agarivorans by Fucoidan extracted from macroalgae

“…Fucoidan Puri cation Method 2 -In the second method, puri cation of freeze-dried fucoidan fractions from Cystoseira sp. and Padina pavonica were carried out according to Yamazaki et al (2016) with some modi cations. Dried fucoidan pellets of Cystoseira sp.…”

Optimization and Prevalidation of TLC-Densitometry Method for Fucoidan Analysis in Sargassum sp. Aqueous Extract

“…Therefore, an analytical method for fucoidan analysis must be developed. Several analytical methods for fucoidan analysis have been developed, including fluorometric assays and UV spectrophotometry (Venkatesan et al, 2018;Yamazaki et al, 2016). However, because herbal extracts are composed of several substances, they are not likely to be simple to apply, and the process requires the isolation of fucoidan from the other components.…”

Injectable Thermosensitive Chitosan-Collagen Hydrogel as A Delivery System for Marine Polysaccharide Fucoidan

“…Consequently, it is also challenging to determine the fucoidan release from a hydrogel over time. To prove the incorporation of fucoidan into the hydrogel, a protocol published by Yamazaki and colleagues [34] was adapted. Yamazaki and colleagues demonstrated that the nucleic acid stain SYBR Gold can be used to quantify the concentration of fucoidan solutions.…”

“…Figure 5. Fucoidan detection inside the hydrogel and fucoidan release tocol from Yamazaki and colleagues [34] was adapted to detect fucoid SYBR Gold nucleic acid stain allowed detecting different fucoidan amo Hydrogels with and without fucoidan were incubated in PBS for 6 days the hydrogel was detected on day 0, 2, and 6 using SYBR Gold. Expe times (n = 3).…”

“…Fucoidan incorporation into the hydrogel was proven using the SYBR Gold Nucleic Acid Gel Stain (Invitrogen, Eugene, Oregon, USA) using an adapted protocol from Yamazaki and colleagues [34]. One-hundred µL sol was pipetted into a 96-well plate and gelated at 37 • C for 30 min.…”