A Fluorescent Sensor-Assisted Paper-Based Competitive Lateral Flow Immunoassay for the Rapid and Sensitive Detection of Ampicillin in Hospital Wastewater

Lin, Hung‐Mo; Fang, Feixiang; Zang, Jiahui; Su, Jianlong; Tian, Qingyuan; Kankala, Ranjith Kumar; Lin, Xuexia

doi:10.3390/mi11040431

Cited by 14 publications

(9 citation statements)

References 30 publications

(18 reference statements)

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“…e assay had a limit of detection of 3.71 U/ml and correlated with ELISA. A DNA aptamer for residual penicillin antibiotic ampicillin was prepared by Lin and coworkers [51]. e researchers used hexachlorofluorescein for oligonucleotide labeling and were able to detect ampicillin in a range of 10 to 200 ng/l and a limit of quantification of 2.71 ng/l when water sample was analyzed.…”

Section: Current Trends In Lft Construction In Point-of-care Diagnosismentioning

confidence: 99%

Point-of-Care Diagnoses and Assays Based on Lateral Flow Test

Pohanka

2021

International Journal of Analytical Chemistry

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Analytical devices for point-of-care diagnoses are highly desired and would improve quality of life when first diagnoses are made early and pathologies are recognized soon. Lateral flow tests (LFTs) are such tools that can be easily performed without specific equipment, skills, or experiences. This review is focused on the use of LFT in point-of-care diagnoses. The principle of the assay is explained, and new materials like nanoparticles for labeling, new recognition molecules for interaction with an analyte, and new additional instrumentation like signal scaling by a smartphone camera are described and discussed. Advantages of the LFT devices as well as their limitations are described and discussed here considering actual papers that are properly cited.

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Section: Current Trends In Lft Construction In Point-of-care Diagnosismentioning

confidence: 99%

Point-of-Care Diagnoses and Assays Based on Lateral Flow Test

Pohanka

2021

International Journal of Analytical Chemistry

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“…Thus, the next experiment was taken with PBS buffer (pH = 7.4). Furth the concentration of detection probes was another key factor in the accuracy of th In our previous work [27], it was found that 500 nM was the most suitable, so nM of detection probe was chosen in this work.…”

Section: Optimization Of Lfa Assay Parametersmentioning

confidence: 99%

“…Furthermore, the concentration of detection probes was another key factor in the accuracy of the assay. In our previous work [27], it was found that 500 nM was the most suitable, so then 500 nM of detection probe was chosen in this work. To achieve the optimal conditions for the quantitative determination of AMP and KAM, the type of NC membrane, the distances between each test line, the distances between the internal line and the conjugate pad, and the detection time were investigated The capture DNAs were immobilized on their corresponding lines.…”

Section: Optimization Of Lfa Assay Parametersmentioning

confidence: 99%

“…For the determination of antibiotic residues, a 21-mer nucleotide kanamycin (KAM) aptamer (Kd ≈ 78.8 nM) [24] and three kinds of nucleotide ampicillin (AMP) aptamers (Kd ≈ 10 nM) have been used for the analysis of AMP [25]. In our previous work, a fluorescent-based LFA strip was fabricated for the detection of AMP residue, and a KAM aptasensor was made based on the use of luminescent carbon nanodots [26,27]. In another case, Rozlosnik and colleagues utilized the combination of an aptamer-assisted electrochemical method and microfluidic technology to develop a method for the determination of AMP and KAM [28].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Quantification of Ampicillin and Kanamycin in Water Samples Based on Lateral Flow Aptasensor Strip with an Internal Line

et al. 2021

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In this work, a simple and rapid method based on the lateral flow assay (LFA) has been developed for the detection of dual antibiotics. To achieve the quantitative assay and to reduce the non-specific adsorption, an internal system has been developed. A non-specific DNA was exploited as an internal standard and could be recognized by the DNA marker that was coated at the internal line. Two different kinds of aptamers were applied to recognize ampicillin (AMP) and kanamycin (KAM), and the distance between the detection line and conjugate pad was then optimized. Under the optimum conditions, the quantitative assays of AMP (R2 = 0.984) and KAM (R2 = 0.990) were achieved with dynamic ranges of 0.50 to 500.0 ng/L, and of 0.50 to 1000.0 ng/L, respectively. The LOQs of AMP and KAM were 0.06 ng/L and 0.015 ng/L, respectively. Finally, the proposed method has been successfully applied to analyze aquaculture water, tap water, and lake water, and hospital wastewater, indicating the established method could be used to monitor the environment.

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“…More than 40 pharmaceuticals were authenticated in 96-well plates using a seven-sensor colorimetric array [34]. A penicillin antibiotic ampicillin was detected using colorimetric [35][36][37] and fluorimetric [38][39][40] techniques. Aggregational, enzymatic, and immune methods with colorimet-ric detection were developed for another penicillin compound, benzylpenicillin [32,41].…”

Section: Introductionmentioning

confidence: 99%

Carbocyanine-Based Fluorescent and Colorimetric Sensor Array for the Discrimination of Medicinal Compounds

et al. 2022

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Array-based optical sensing is an efficient technique for the determination and discrimination of small organic molecules. This study is aimed at the development of a simple and rapid strategy for obtaining an optical response from a wide range of low-molecular-weight organic compounds. We have suggested a colorimetric and fluorimetric sensing platform based on the combination of two response mechanisms using carbocyanine dyes: aggregation and oxidation. In the first one, the analyte forms ternary aggregates with an oppositely charged surfactant wherein the dye is solubilized in the hydrophobic domains of the surfactant accompanied with fluorescent enhancement. The second mechanism is based on the effect of the analyte on the catalytic reaction rate of dye oxidation with H2O2 in the presence of a metal ion (Cu2+, Pd2+), which entails fluorescence waning and color change. The reaction mixture in a 96-well plate is photographed in visible light (colorimetry) and the near-IR region under red light excitation (fluorimetry). In this proof-of-concept study, we demonstrated the feasibility of discrimination of nine medicinal compounds using principal component analysis: four cephalosporins (ceftriaxone, cefazolin, ceftazidime, cefotaxime), three phenothiazines (promethazine, promazine, chlorpromazine), and two penicillins (benzylpenicillin, ampicillin) in an aqueous solution and in the presence of turkey meat extract. The suggested platform allows simple and rapid recognition of analytes of various nature without using spectral equipment, except for a photo camera.

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A Fluorescent Sensor-Assisted Paper-Based Competitive Lateral Flow Immunoassay for the Rapid and Sensitive Detection of Ampicillin in Hospital Wastewater

Cited by 14 publications

References 30 publications

Point-of-Care Diagnoses and Assays Based on Lateral Flow Test

Point-of-Care Diagnoses and Assays Based on Lateral Flow Test

Simultaneous Quantification of Ampicillin and Kanamycin in Water Samples Based on Lateral Flow Aptasensor Strip with an Internal Line

Carbocyanine-Based Fluorescent and Colorimetric Sensor Array for the Discrimination of Medicinal Compounds

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