A fluorescent probe for rapid aqueous fluoride detection and cell imaging

Abstract

A stable and highly selective fluorescent probe has been designed and synthesized for the rapid detection of fluoride ions (F−) in aqueous solution and living cells. The design was based on the high reactivity of F− toward a silyl group.

Novel 2,1,3-benzothiadiazole derivatives used as selective fluorescent and colorimetric sensors for fluoride ion

“…In this investigation, two compounds, 7-(9-octyl-9H-carbazol-3-yl)- [1,2,5]thiadiazol-4-amine (CBT-NH) and 7-(10-octyl-10H-phenothiazin-3-yl)-N-(4-nitrophenyl)-benzo[c] [1,2,5]thiadiazol-4-amine (PBT-NH) (Scheme 1), were designed and synthesized, and used as fluorescent sensors for fluoride with the detection limits 0.86uM and 4.25uM, respectively. And their quantum yields were 0.034 and 0.015, respectively.…”

Visualizing Fluoride Ion in Mitochondria and Lysosome of Living Cells and in Living Mice with Positively Charged Ratiometric Probes

“…Recently, several water soluble fluorescence probes for fluoride detection have been developed by attachment of sugar moiety 28,31 . We also reported fluorescent fluoride chemosensors with attachment of quaternary ammonium which greatly increase the solubility of probes in water, and quaternary ammonium itself could sequester fluoride anion due to charge attraction and promote the desilylation of probes 40 .…”

“…In the past decade, several fluorescent probes for detection of fluoride ion were developed in solution or cell imaging [15][16][17] . They are mainly based on fluoridehydrogen bonding 18-20 , boron-fluoride or stibium-fluoride complexation [21][22][23][24] , and desilylation [25][26][27][28][29][30][31][32][33][34][35][36][37][38][39] . However, most fluorescent probes for fluoride ion still have some limitations in detection and bioimaging.…”

Selective recognition and discrimination of H2PO4− and F− based on a cleft-shaped anion receptor incorporating bisamide and bispyrrole groups

“…Phosphate and its derivatives played an important part in information processing, signal transduction and energy storage of biological system (10). Many elaborate anion receptors or sensors capable of selective recognition of one of the above two anions had been reported (11)(12)(13)(14)(15)(16)(17)(18)(19). However, the examples of the use of a single receptor for the simultaneous discrimination of F 2 and H 2 PO 4 2 were still very lacking (20)(21)(22)(23)(24), due to the subtle difference in the basicity and surface charge density between them (25,26).…”