A fluorescent probe for alkaline phosphatase via excited state intramolecular proton transfer

Hu, Qinghua; Zeng, Fang; Wu, Shuizhu

doi:10.1016/j.snb.2015.05.111

Cited by 33 publications

(23 citation statements)

References 74 publications

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“…Probe 65 could be used to detect endogenous ALP levels in human serum samples and to be internalised into HeLa cells where it could be used to image endogenous ALP activity. 188 Han et al have developed two HBT-based fluorescent probes 66-AMP functionalised with the nucleotides adenosine monophosphate (AMP) and 66-GMP guanosine monophosphate (GMP), with the aim of achieving greater selectivity for phosphatase activity (Scheme 63). Initially, both probes displayed enol emission due to the ESIPT process being blocked.…”

Section: Phosphatasesmentioning

confidence: 99%

Excited-state intramolecular proton-transfer (ESIPT) based fluorescence sensors and imaging agents

et al. 2018

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Section: Phosphatasesmentioning

confidence: 99%

Excited-state intramolecular proton-transfer (ESIPT) based fluorescence sensors and imaging agents

et al. 2018

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“…Fluorescent probes based on the intramolecular proton transfer (ESIPT) mechanism have valuable advantages such as strong luminescence, a large Stokes shift, good light stability, and good sensing performance. Recently, numerous theoretical and experimental studies on ESIPT have emerged 17,38‐41 . Generally speaking, molecules that contain hydrogen bond donors (OH or NH 2 ) and hydrogen bond receptors (N and CO) can generate ESIPT fluorescence 17 …”

Section: Molecular Fluorescent Probesmentioning

confidence: 99%

Applications of fluorescent materials in the detection of alkaline phosphatase activity

Guo

Cui

2020

J Biomed Mater Res

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Alkaline phosphatase (ALP) is important in the diagnosis of many diseases. Because ALP is used to detect biomarkers for many diseases, many researchers conduct investigations to develop ALP detection strategies. The use of fluorescent material has attracted attention because of the technique's high sensitivity and the low sample volume required. Herein, we review and discuss the working mechanisms and advantages of four main categories:DNA fluorescent probes, molecular fluorescent probes, chemical coordination-based probes, and nanoparticle probes. Development prospects and trends are also discussed.

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“…Several turn‐on ( 11 , 12 , 13 , and 14 , Figure ) as well as ratiometric ( 15 , 16 , 17 , and 18 , Figure ) probes for ALP have been described. For example, 11 , was used to monitor endogenous ALP activity in real‐time in HeLa cells.…”

Section: Small‐molecule Reportersmentioning

confidence: 99%

Interrogating Protein Phosphatases with Chemical Activity Probes

Casey

Stains

2018

Chemistry A European J

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Protein phosphatases, while long overlooked, have recently become appreciated as drivers of both normal- and disease-associated signaling events. As a result, the spotlight is now turning torwards this enzyme family and efforts geared towards the development of modern chemical tools for studying these enzymes are well underway. This Minireview focuses on the evolution of chemical activity probes, both optical and covalent, for the study of protein phosphatases. Small-molecule probes, global monitoring of phosphatase activity through the use of covalent modifiers, and targeted fluorescence-based activity probes are discussed. We conclude with an overview of open questions in the field and highlight the potential impact of chemical tools for studying protein phosphatases.

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A fluorescent probe for alkaline phosphatase via excited state intramolecular proton transfer

Cited by 33 publications

References 74 publications

Excited-state intramolecular proton-transfer (ESIPT) based fluorescence sensors and imaging agents

Excited-state intramolecular proton-transfer (ESIPT) based fluorescence sensors and imaging agents

Applications of fluorescent materials in the detection of alkaline phosphatase activity

Interrogating Protein Phosphatases with Chemical Activity Probes

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