A Fluorescent Probe Based on Polyethyleneimine Protected Copper Nanoclusters for the Assay of Tetracycline Hydrochloride and Vitamin B12

Abstract

A water-soluble and polyethyleneimine protected copper nanoclusters (CuNCs@PEI) was prepared by one-step ultraviolet radiation (UVI) combined with microwave heating (MWH) method, and the influence of different energy suppliers on the CuNCs@PEI luminescence performance was investigated in detail. The as-prepared CuNCs@PEI could be used as a viable fluorescent probe for sensitive and selective mensurating TCH (tetracycline hydrochloride)/VB12 (vitamin B12) with a linear response in the range of 0.33-66.67/0.33-53.33 μmol L À 1 , and corresponding limit of detection (LOD) and limit of quantifica-tion (LOQ) were calculated to be 55.50/56.34 and 184.82/ 187.61 nmol L À 1 . The fluorescence (FL) quenching of CuNCs@PEI by TCH/VB12 came from the combination of FRET (Förster resonance energy transfer) and IFE (inner filter effect). Moreover, the probe was successfully extended to the assays of TCH in veterinary powder or TCH tablets and VB12 in oral liquid or VB12 tablets with satisfactory results compared to the standard HPLC method. Besides, the CuNCs@PEI probe exhibited a good temperature sensing.

Detection of Amaranth and Iron in Foods by Modified Carbon Quantum Dots as a Ratiometric Fluorescence Probe

“…In Eq. (1), the meanings represented by each parameter were given in the literature [42] . Immediately afterwards, the quenching rates of G‐CDs observations ( E obsd ) and corrected ( E cor ) fluorescence intensities for the addition of different concentrations of AM were observed (Figure 8b).…”

“…( 1), the meanings represented by each parameter were given in the literature. [42] Immediately afterwards, the quenching rates of G-CDs observations (E obsd ) and corrected (E cor ) fluorescence intensities for the addition of different concentrations of AM were observed (Figure 8b). The IFE inhibited the G-CDs-AM system with an efficiency of 81 %, indicating that the fluorescence quenching of G-CDs by AM was mainly attributed to the IFE.…”

Recent Advances in Dietary Sources, Health Benefits, Emerging Encapsulation Methods, Food Fortification, and New Sensor-Based Monitoring of Vitamin B12: A Critical Review

“…Additionally, our probe was successfully applied to assess TCH in veterinary powder or TCH tablets, as well as VB 12 in oral liquid or VB 12 tablets, yielding satisfactory results when compared to the standard HPLC method. The CuNCs@PEI probe also demonstrated reliable temperature-sensing capabilities [168]. Shanmugaraj et al successfully prepared histidine-stabilized copper nanoclusters (His-CuNCs) through a straightforward and eco-friendly method, avoiding the use of toxic organic solvents.…”

Ratiometric Detection of Al Based on the Mixing of D‐penicillamine‐Functionalized Copper Nanoclusters with Pyridoxal 5’‐phosphate

“…In addition, in order to study the FL quenching mechanism of the DPA-CuNCs-PLP system from the IFE, the IFE was corrected through Eq. ( 1), [63] and corresponding data were calculated and listed in Table S1.…”

“…In addition, in order to study the FL quenching mechanism of the DPA‐CuNCs‐PLP system from the IFE, the IFE was corrected through Eq. (1), [63] and corresponding data were calculated and listed in Table S1. $$$\vcenter{\openup.5em\halign{$\displaystyle{#}$\cr {\rm{CF}} = {{F_{{\rm{cor}}} } \over {F_{{\rm{obsd}}} }} = {{2.3{\rm{d}}A_{{\rm{ex}}} } \over {1 - 10^{ - {\rm{d}}A_{{\rm{ex}}} } }}10^{{\rm{g}}A_{{\rm{em}}} } {{2.3{\rm{s}}A_{{\rm{em}}} } \over {1 - 10^{ - {\rm{s}}A_{{\rm{em}}} } }}\hfill\cr}}$$$ …”