A fluorescent chemodosimeter specific for cysteine: effective discrimination of cysteine from homocysteine

Li, Honglin; Fan, Jiangli; Wang, Jingyun; Tian, Mingliang; Du, Jianjun; Sun, Shiguo; Sun, Pingping; Peng, Xiaojun

doi:10.1039/b907511a

Cited by 330 publications

(109 citation statements)

References 52 publications

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“…So the probe may serve as a powerful molecular tool useful for the estimation of the thiols concentration in biological and clinical studies in living cells ( Figure 6). The different chemodosimeter which can effectively discriminate Cys from Hcy and GSH in living cells with notable fluorescence enhancement was reported by Peng et al [128]. They synthesized the colorless and weakly fluorescent Rhodamine-6G-conjugate including a spirolactam-ring with an unsaturated aldehyde.…”

Section: Fluorescent Dyes As "Molecular Photoswiches" and Molecular Cmentioning

confidence: 99%

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Miksa¹

2016

Med chem (Los Angeles)

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This review highlights the role of fluorescent dyes as active "molecular photoswiches" focusing on the application in bioimaging and anticancer therapies in the field of therapeutic delivery. The author describes the development of prodrugs targeted to specific cell types and polymeric nanocarriers (capsules, micelles and silica nanoparticles) used as fluorescent probes which offer advantages in the integration of "smart" features of fluorescent dyes into synthetic materials. The incorporation of biologically responsive components that cleave upon changes in pH or light irradiation is fundamental to the successful design of such carriers with capabilities to load and release therapeutics specifically at a target site.

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Section: Fluorescent Dyes As "Molecular Photoswiches" and Molecular Cmentioning

confidence: 99%

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Miksa¹

2016

Med chem (Los Angeles)

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“…Based on 3δ blank /k (where δ blank is the standard deviation of the blank solution and k is the slope of the calibration plot), the limit of detection (LOD) was attained of 12.5 nM. A comparison of the analytical performances with that previously reported methods [4][5][6]8,11,13,15,[30][31][32][33][34][35] for the determination of L-Cys is summarized in Table 2. As can be seen from the table, the present method is an ideal candidate for L-Cys analysis.…”

Section: Fluorescent Detection Of L-cys Via Rb1mentioning

confidence: 99%

“…Recent research have focused on the development of direct fluorescent methods for the detection of LCys and other biological thiols, aiming at high sensitivity, remote operation, and suitability as a fluorescent tag for labeling [11,12]. Among them, the fluorescence turn-on mode, either increase in fluorescence intensity or a new photoluminescence at a different wavelength, has been increasingly developed for mercapto biomolecules [13][14][15]. For example, Hao et al [13] demonstrated a fluorescence turn-on molecular probe for direct and highly selective detection of Cys using a zwitterionic dye.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Hao et al [13] demonstrated a fluorescence turn-on molecular probe for direct and highly selective detection of Cys using a zwitterionic dye. Li et al [15] devised and synthesized a chemodosimeter (also named chemosensor [16,17]) which could effectively discriminate Cys from homocysteine and On the other hand, it is imperative to highly efficiently extract and separate trace L-Cys in diverse complex matrices. For this purpose, a promising material, molecularly imprinted polymers (MIPs) can be employed based on its selective recognition and high adsorption capabilities [18].…”

Section: Introductionmentioning

confidence: 99%

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Chemodosimeter-based fluorescent detection of l-cysteine after extracted by molecularly imprinted polymers

Cai

Zhong

et al. 2014

Talanta

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Chemodosimeter Rhodamine B a b s t r a c tA chemodosimeter-based fluorescent detection method coupled with molecularly imprinted polymers (MIPs) extraction was developed for determination of L-cysteine (L-Cys) by combining molecular imprinting technique with fluorescent chemodosimeter. The MIPs prepared by precipitation polymerization with L-Cys as template, possessed high specific surface area of 145 m 2 /g and good thermal stability without decomposition lower than 300 1C, and were successfully applied as an adsorbent with excellent selectivity for L-Cys over other amino acids, and enantioselectivity was also demonstrated. A novel chemodosimeter, rhodamine B1, was synthesized for discriminating L-Cys from its structurally similar homocysteine and glutathione as well as various possibly co-existing biospecies in aqueous solutions with notable fluorescence enhancement when adding L-Cys. As L-Cys was added with increasing concentrations, an emission band peaked at 580 nm occurred and significantly increased in fluorescence intensity, by which the L-Cys could be sensed optically. High detectability up to 12.5 nM was obtained. An excellent linearity was found within the wide range of 0.05-50 μM (r¼ 0.9996), and reasonable relative standard deviations ranging from 0.3% to 3.5% were attained. Such typical features as high selectivity, high sensitivity, easy operation and low cost enabled this MIPs-fluorometry to be potentially applicable for routine detection of trace L-Cys.

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“…As the fluorometric method can reach a very low detection limit, considerable affords have been devoted to develop fluorescent probe for mercapto biomolecules in recent years [15]- [20]. However, owing to the similarity in structure and reactivity of Cys, Hcy, and GSH, only few fluorescent sensors with ability to discriminate these biomolecules have been reported so far [21]- [25].…”

Section: Introductionmentioning

confidence: 99%

Development of Fluorescent Phycocyanin-Cu2+ Chemosensor for Detection of Homocysteine

Puangploy¹,

Oaew²,

Surareungchai³

2015

IJBBB

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Abstract:A simple optical chemosensor from phycocyanin (L1), a protein-pigment complex extracted from cyanobacterium Spirulina platensis, has been developed. The L1 shows high selectivity toward Cu 2+ by exhibiting spectral shift and quenching of fluorescence intensity. This L1-Cu 2+ complex, so called ensemble probe, can be used to detect mercapto biomolecules, and has superior affinity toward homocysteine (Hcy). Upon binding to mercapto biomolecules, the color change from colorless to blue and the fluorescence emission change from colorless to pink were observed. This chemosensor could detect Hcy at concentration as low as 4.71 µM (S/N = 3) with a linear range of 10-100 µM (R 2 = 0.9903). This sensing approach may have found applications in clinical diagnostics.

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A fluorescent chemodosimeter specific for cysteine: effective discrimination of cysteine from homocysteine

Cited by 330 publications

References 52 publications

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Chemodosimeter-based fluorescent detection of l-cysteine after extracted by molecularly imprinted polymers

Development of Fluorescent Phycocyanin-Cu2+ Chemosensor for Detection of Homocysteine

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