A fluorescent amplification strategy for high-sensitive detection of 17 β-estradiol based on EXPAR and HCR

Wang, Yu; Zhao, Xudong; Zhang, Man; Sun, Xuan; Bai, Jintao; Peng, Yuan; Li, Shuang; Han, Dianpeng; Ren, Shuyue; Wang, Jiang; Han, Tao; Gao, Yifei; Ning, Baoan; Gao, Zhixian

doi:10.1016/j.aca.2020.04.010

Cited by 30 publications

(29 citation statements)

References 33 publications

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“…Sequence information for the nucleic acids used in this study. Table S2 52 1 × 10 3 to 1 × 10 −3 1 × 10 −3 water photocatalytic fuel cell (PFC) platform 53 1−500 0.12 lake water, serum fluorescent amplification 13 0.00147−2.94 1.36 × 10 −3 water, milk LC−MS/MS 12 to 8.8 × 10 −3 1.34 × 10 −3 water electrochemical immunosensor 10 to 12 4.41 × 10 −3 water aptamer-based optical biosensor 54 5−400 0.2 water label-free fluorescent aptasensor 15 5 × 10 4 to 1 × 10 6 37 fetal bovine serum label-free electrochemical aptasensor 57 1 × 10 −6 to 1 × 10 3 1 × 10 −6 human urine ultrasensitive colorimetric detection 55 5−400 0.2 water, urine lateral flow assay test strip (LFA) 56 50−1000…”

Section: ■ Associated Contentmentioning

confidence: 99%

“…Electrochemical sensors and liquid chromatography-tandem mass spectrometry (LC–MS/MS) have high sensitivity, accuracy, and robustness but require a complex sample preparation process and high costs. Aptamer-based detection , and quantum dots methods do not require special equipment, and their operations are relatively simple, while they need a long time and have poor repeatability. Therefore, an ultrasensitive detection method is urgently needed to be proposed.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with the conventional polymerase chain reaction (PCR) technology, nucleic acids isothermal amplification is a simple process in which nucleic acid sequences are rapidly and efficiently accumulated at a constant temperature . It is widely used in the detection of tumor markers, DNA or RNA virus toxins, and small-molecule targets. − At present, isothermal amplification technology has been very mature, including loop-mediated nucleic acid isothermal amplification technology (LAMP), , strand replacement isothermal amplification technology (SDA), , rolling circle amplification (RCA), − hybrid chain reaction amplification (HCR), , exponential amplification (EXPAR), , and other nucleic acid amplification technologies. Different isothermal amplification techniques have their own characteristics in primer design, reaction conditions, and products.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrasensitive Detection of 17β-Estradiol (E2) Based on Multistep Isothermal Amplification

Wang

Peng²,

Wu³

et al. 2021

Anal. Chem.

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17β-Estradiol (E2) can cause an adverse effect on the human endocrine system even at the nanomolar level. Measurements of very low levels of E2 remain a critical challenge due to insufficient sensitivity. In this study, a multistep isothermal amplification fluorescence strategy was constructed, which could realize the exponential amplification of target E2. Specifically, strand displacement reaction (SDA), rolling circle amplification (RCA), and multiprimed rolling circle amplification (MRCA) were combined in a series to quantify trace complementary strand of E2 (cDNA). The E2 aptamer and cDNA were hybridized and modified on the magnetic beads. E2 could bind to its aptamer and cause the release of the cDNA. Then, cDNA would combine with the template DNA, initiating the SDA−RCA−MRCA. The molecular beacons, possessing low background signal, whose fluorescence was quenched in the state of chain folding, could be specifically recognized by the long single-stranded DNA (L-ssDNA) generated by the multistep isothermal amplification triggered by cDNA, and then the fluorescence of the molecular beacons could be restored. Therefore, the E2 could be quantitatively detected by the recovery fluorescence intensity. The fluorescence value showed a good linear relationship with the concentration of E2 in the range of 0.001836−183.6 nM, and the limit of detection (LOD) was as low as 63.09 fM. In addition, the recovery rates of this method spiked in milk and water were 80.8−107.0%, respectively. This method has the advantage of multistep isothermal amplification to obtain abundant fluorescence signals, which may provide a new possibility for highly sensitive detection of other small-molecule targets.

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Section: ■ Associated Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrasensitive Detection of 17β-Estradiol (E2) Based on Multistep Isothermal Amplification

Wang

Peng²,

Wu³

et al. 2021

Anal. Chem.

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“…You et al developed a colorimetric and fluorescent dual-mode immunochromatographic strip for PSA in whole blood, and the cut-off value of this immunoassay was 1.07 pg/mL, which is at least two orders of magnitude lower than the conventional fluorescence immunoassay [ 25 ]. Noble metal nanoparticles [ 26 , 27 , 28 ], quantum dots (QDs) [ 29 , 30 ], and up-conversion nanoparticles [ 31 , 32 ], etc., are often used as sensitive signal probes. QDs, also known as semiconductor nanocrystals, can provide high fluorescence quantum yields.…”

Section: Introductionmentioning

confidence: 99%

A Polydopamine-Coated Gold Nanoparticles Quenching Quantum Dots-Based Dual-Readout Lateral Flow Immunoassay for Sensitive Detection of Carbendazim in Agriproducts

et al. 2022

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In this study, a fluorometric and colorimetric dual-readout lateral flow immunoassay (LFIA) using antibody functionalized polydopamine-coated gold nanoparticles (Au@PDAs) as a probe was developed for the detection of carbendazim (CBD). Colloidal gold nanoparticles (AuNPs) were coated with polydopamines (PDA) by the oxidation of dopamine to synthesize Au@PDA nanoparticles. The Au@PDA nanoparticles mediated ZnCdSe/ZnS quantum dots (QDs) fluorescence quenching and recovery, resulting in a reverse fluorescence enhancement detection format of CBD. The CBD detection was obtained by the competition between the CBD and the immobilized antigen for Au@PDAs labelled antibody binding, resulting in a significant fluorescence increase and colorimetry decrease corresponded to the concentration of CBD. Dual readout modes were incorporated into the LFIA using the colorimetry signal under natural light and the fluorescence signal under UV light. The cut-off value in the mode of the colorimetric signal and fluorometric signal for CBD detection was 0.5 μg/mL and 0.0156 μg/mL, respectively. The sensitivity of LFIA of the fluorescence mode was 32 times higher than that of the colorimetry mode. There was negligible cross reactivity obtained by using LFIA for the determination of thiabendazole, benomyl, thiophanate-methyl, and thiophanate-ethyl. Consistent and satisfactory results have been achieved by comparing the results of Au@PDAs-QDs-LFIA and liquid chromatography-tandem mass spectrometry (LC—MS/MS) testing spiked cucumber and strawberry samples, indicating good reliability of the Au@PDAs-QDs-LFIA.

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“…In view of these limitations, DNA-based signal amplification methods have been developed to enhance the sensitivity of protein-specific glycosylation analysis , and include rolling circle amplification, − proximity ligation assays, , and exonuclease III-aided recycling hybridization. , These amplification methods require the involvement of enzymes, which may not be suitable for living conditions. Therefore, a nonenzymatic amplification technique, the hybridization chain reaction (HCR), which can provide multiple, isothermal, and enzyme-free molecular signals, is increasingly being used to study live-cell glycoconjugates. − According to reports, a proximity-induced HCR strategy has been developed to visualize protein-specific glycosylation in living cells and living organisms, such as zebrafish . Nevertheless, fluorescence-based methods are limited to low tissue penetration, thereby impeding further extension to imaging techniques. − …”

mentioning

confidence: 99%

Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice

et al. 2021

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Glycosylation is a key cellular mechanism that regulates several physiological and pathological functions. Therefore, identification and characterization of specific-protein glycosylation in vivo are highly desirable for studying glycosylation-related pathology and developing personalized theranostic modalities. Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo. Two kinds of functional DNA probes were designed. A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition. Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain. Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals. Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment. This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.

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A fluorescent amplification strategy for high-sensitive detection of 17 β-estradiol based on EXPAR and HCR

Cited by 30 publications

References 33 publications

Ultrasensitive Detection of 17β-Estradiol (E2) Based on Multistep Isothermal Amplification

Ultrasensitive Detection of 17β-Estradiol (E2) Based on Multistep Isothermal Amplification

A Polydopamine-Coated Gold Nanoparticles Quenching Quantum Dots-Based Dual-Readout Lateral Flow Immunoassay for Sensitive Detection of Carbendazim in Agriproducts

Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice

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