A fluorescence study of the loading and time stability of doxorubicin in sodium cholate/PEO-PPO-PEO triblock copolymer mixed micelles

Tasca, Elisamaria; Giudice, Alessandra Del; Galantini, Luciano; Schillén, Karin; Giuliani, Anna Maria; Giustini, Mauro

doi:10.1016/j.jcis.2019.01.075

Cited by 25 publications

(24 citation statements)

References 48 publications

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“…DOX is one of the widely used powerful antineoplastic agents in clinical practice for the treatment of various cancers, hematological malignancies, soft tissue sarcomas, and solid tumors [ 19 ]. In order to increase its solubility in water, it is generally used in its hydrochloride form [ 20 ]. Unfortunately, the use of DOX revealed significant tissue toxicities, such as acute and chronic cardiotoxicity, which frequently contribute to fatal congestive heart failure.…”

Section: Introductionmentioning

confidence: 99%

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Gagliardi

Cosco

Udongo³

et al. 2020

Pharmaceutics

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Glyceryl monooleate (GMO) is one of the most popular amphiphilic lipids, which, in the presence of different amounts of water and a proper amount of stabilizer, can promote the development of well defined, thermodynamically stable nanostructures, called lyotropic liquid crystal dispersions. The aim of this study is based on the design, characterization, and evaluation of the cytotoxicity of lyotropic liquid crystal nanostructures containing a model anticancer drug such as doxorubicin hydrochloride. The drug is efficiently retained by the GMO nanosystems by a remote loading approach. The nanostructures prepared with different non-ionic surfactants (poloxamers and polysorbates) are characterized by different physico-chemical features as a function of several parameters, i.e., serum stability, temperature, and different pH values, as well as the amount of cryoprotectants used to obtain suitable freeze-dried systems. The nanostructures prepared with poloxamer 407 used as a stabilizer show an increased toxicity of the entrapped drug on breast cancer cell lines (MCF-7 and MDA-MB-231) due to their ability to sensitize multidrug-resistant (MDR) tumor cells through the inhibition of specific drug efflux transporters. Moreover, the interaction between the nanostructures and the cells occurs after just a few hours, evidencing a huge cellular uptake of the nanosystems.

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Section: Introductionmentioning

confidence: 99%

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Gagliardi

Cosco

Udongo³

et al. 2020

Pharmaceutics

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“…[64][65][66] Our studies were driven by the fact that these copolymers could have a potential use as BS sequestrants in the therapy of bile-acid related diseases including hypercholesterolemia and as smart nanocarriers of active molecules. 67 In ref. [64][65][66] we demonstrated that NaGDC associates to PEO-PPO-PEO micelles to form mixed complexes through hydrophobic interactions.…”

Section: Introductionmentioning

confidence: 99%

Block copolymers as bile salt sequestrants: intriguing structures formed in a mixture of an oppositely charged amphiphilic block copolymer and bile salt

Schillén

Galantini

et al. 2019

Phys. Chem. Chem. Phys.

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“…[2] Very active research started very soon to try to overcome these drawbacks, and it is still alive, trying to find new formulations or new administration strategies. [1][3] [4] A C C E P T E D M A N U S C R I P T From a physico-chemical point of view, DX is an extremely intriguing molecule. Despite its relatively low molar mass (580 g/mol), it exhibits features typical of high molecular weight species, such as the capability to act as a water gelator.…”

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confidence: 99%

The self-association equilibria of doxorubicin at high concentration and ionic strength characterized by fluorescence spectroscopy and molecular dynamics simulations

Tasca

Alba

Galantini

et al. 2019

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Graphical abstract ABSTRACTThe self-association equilibria of doxorubicin hydrochloride (DX), at high drug and NaCl concentrations, are studied by temperature scan fluorescence spectroscopy, with the support of molecular dynamics (MD) calculations. Even though all anthracyclines show dimerization equilibria, DX only can further associate into long polymeric chains according to DXmon ⇄ DXdim ⇄ DXpol. This is reflected not only in the mechanical properties of DXpol solutions (behaving as thixotropic gels) but also in their spectroscopic behaviour. Fluorescence, in particular, is the technique of election to study this complex set of equilibria. Upon increasing the temperature, DXpol melts into DXdim, which in turn is in equilibrium with DXmon. Since DXdim is non fluorescent, with a fluorescence temperature scan experiment the DXpol⇄ DXmon equilibrium is probed. However, also information on the DX dimerization equilibrium can be derived together with the relevant thermodynamic parameters ruling the dimerization process (ΔH dim°= −56 kJ mol −1 ; ΔS dim°= −97 J mol −1 K −1 ). The residence time of DX molecules in the dimer (74.7 μs), as well as the monomers mutual orientation in the dimer, are characterized by means of theoretical and computational modelling.

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A fluorescence study of the loading and time stability of doxorubicin in sodium cholate/PEO-PPO-PEO triblock copolymer mixed micelles

Cited by 25 publications

References 48 publications

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Block copolymers as bile salt sequestrants: intriguing structures formed in a mixture of an oppositely charged amphiphilic block copolymer and bile salt

The self-association equilibria of doxorubicin at high concentration and ionic strength characterized by fluorescence spectroscopy and molecular dynamics simulations

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