A fluorescence strategy for monitoring α-glucosidase activity and screening its inhibitors from Chinese herbal medicines based on Cu nanoclusters with aggregation-induced emission

Li, Cong; Zi, Yuqiu; Xu, Dawei; Jiang, Dafeng; Qü, Fei

doi:10.1007/s00216-021-03214-w

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…Li et al [157] recently developed a novel fluorimetric screening assay in which 1-dodecanethiol-capped Cu nanoclusters were used to monitor AG activity and measure its inhibition by test samples. A visual device for high-throughput screening was described, involving the immobilization of the nanoclusters on agarose hydrogels in polyethylene tubes.…”

Section: Recent Developments In Agi Screeningmentioning

confidence: 99%

“…Although the biological type of AG was not stated, the strategy was more sensitive for screening AGIs than the common colorimetric method using PNPG. The fluorescence-based assay allows for the elimination of potentially confounding background absorbance from phyto-extract constituents, which is a notable advantage over the colorimetric approach [157]. Lankatillake et al [158] recommended the routine use of sample and substrate blanks in spectroscopic AG inhibition assays to account for interference from background absorbance/ fluorescence.…”

Section: Recent Developments In Agi Screeningmentioning

confidence: 99%

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Critical Assessment of In Vitro Screening of α-Glucosidase Inhibitors from Plants with Acarbose as a Reference Standard

Miller

2021

Planta Med

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Postprandial hyperglycemia is treated with the oral antidiabetic drug acarbose, an intestinal α-glucosidase inhibitor. Side effects of acarbose motivated a growing number of screening studies to identify novel α-glucosidase inhibitors derived from plant extracts and other natural sources. As “gold standard”, acarbose is frequently included as the reference standard to assess the potency of these candidate α-glucosidase inhibitors, with many outperforming acarbose by several orders of magnitude. The results are subsequently used to identify suitable compounds/products with strong potential for in vivo efficacy. However, most α-glucosidase inhibitor screening studies use enzyme preparations obtained from nonmammalian sources (typically Saccharomyces cerevisiae), despite strong evidence that inhibition data obtained using nonmammalian α-glucosidase may hold limited value in terms of identifying α-glucosidase inhibitors with actual in vivo hypoglycemic potential. The aim was to critically discuss the screening of novel α-glucosidase inhibitors from plant sources, emphasizing inconsistencies and pitfalls, specifically where acarbose was included as the reference standard. An assessment of the available literature emphasized the cruciality of stating the biological source of α-glucosidase in such screening studies to allow for unambiguous and rational interpretation of the data. The review also highlights the lack of a universally adopted screening assay for novel α-glucosidase inhibitors and the commercial availability of a standardized preparation of mammalian α-glucosidase.

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Section: Recent Developments In Agi Screeningmentioning

confidence: 99%

Section: Recent Developments In Agi Screeningmentioning

confidence: 99%

Critical Assessment of In Vitro Screening of α-Glucosidase Inhibitors from Plants with Acarbose as a Reference Standard

Miller

2021

Planta Med

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“…Fluorescence analysis has been widely explored for α-glucosidase activity and diverse α-glucosidase inhibitor sensing, thanks to its superior sensitivity and simplicity. For example, fluorescent CDs, , Cu nanoclusters with aggregation-induced emission, and Boe@GSH-CuNCs have been applied to detect α-glucosidase activity by interacting with the generated absorber/quencher. Obviously, ratiometric fluorescence methods, which require building a device for light excitation, exhibit favorable accuracy attributed to their built-in self-calibration which corrects a variety of analyte-independent factors .…”

Section: Introductionmentioning

confidence: 99%

Lumi-HOF@Tb as Probes for Multiple Ratiometric Fluorescence and Chemiluminescence Sensing of α-Glucosidase

Zhang

Chen

et al. 2022

Anal. Chem.

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The innovative assembly of luminescent hydrogen-bonded organic frameworks (HOFs) into multifunctional optical sensors is of great significance for developing advanced materials. Herein, we report a facile room-temperature synthesis strategy for the luminol HOF modified by Tb3+ (Lumi-HOF@Tb) and featuring sensitive chemiluminescence and fluorescence characteristics. Lumi-HOF@Tb is further pioneered as a dual-signal sensor for selective detection of α-glucosidase, a type of enzyme that plays a crucial role in the digestion of carbohydrates, and screening of its inhibitors. The sensor is constructed by combining the dual optical characteristics of luminol from the HOF and lanthanide ion assistance. From the hydrolysis of α-glucosidase and the 4-nitrophenyl-α-d-glucopyranoside (pNGP) substrate emerges the fluorescent luminol–p-nitrophenol (pNP) complex at 466 nm and changes the inner filter absorption to recover Tb3+ characteristic fluorescence at 546 nm; luminol also produces a chemiluminescence signal driven by H2O2 from additional glucose oxidase-catalyzed hydrolysis of α-d-glucose. Fluorescence and chemiluminescence assays for α-glucosidase activity have therefore been established and exhibit detection limits as low as 0.04 and 0.005 U L–1, respectively. This study not only presents the possibility of Ln3+-HOF-based sensors as intelligent optical materials by integration of fluorescence and chemiluminescence techniques but also demonstrates great potential for future applications in biosensing.

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Fluorescent metal nanoclusters: From luminescence mechanism to applications in enzyme activity assays

Wang,

Tan,

Wang

et al. 2024

Biosensors and Bioelectronics

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A fluorescence strategy for monitoring α-glucosidase activity and screening its inhibitors from Chinese herbal medicines based on Cu nanoclusters with aggregation-induced emission

Cited by 6 publications

References 34 publications

Critical Assessment of In Vitro Screening of α-Glucosidase Inhibitors from Plants with Acarbose as a Reference Standard

Critical Assessment of In Vitro Screening of α-Glucosidase Inhibitors from Plants with Acarbose as a Reference Standard

Lumi-HOF@Tb as Probes for Multiple Ratiometric Fluorescence and Chemiluminescence Sensing of α-Glucosidase

Fluorescent metal nanoclusters: From luminescence mechanism to applications in enzyme activity assays

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