A fluorescence and CD study on the interaction of synthetic lipophilic hepatitis B virus preS(120-145) peptide analogues with phospholipid vesicles

Cajal, Yolanda; Rabanal, Francesc; Alsina, M. A.; Reig, F.

doi:10.1002/(sici)1097-0282(199605)38:5<607::aid-bip6>3.0.co;2-w

Cited by 30 publications

(18 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Size of liposomes was measured in Malvern Autosizer and was lower than 80 nm in diameter as described earlier. 16,38 Circular Dichroism CD spectra were recorded using a Jobin-Yvon Mark VI dichrograph in quartz cells of optical path 0.02 cm at RT, under constant nitrogen flush. The dichrograph was calibrated with D-(Ϫ)-pantoyl-lactone at ϭ 220 nm and with epiandrosterone at ϭ 304 nm.…”

Section: Preparation Of Liposomesmentioning

confidence: 93%

Effect of phospholipid bilayers on solution conformation of branched polymeric polypeptides and peptide-polymer conjugates

Nagy¹,

Májer

Hudecz

2000

Biopolymers

View full text Add to dashboard Cite

Section: Preparation Of Liposomesmentioning

confidence: 93%

Effect of phospholipid bilayers on solution conformation of branched polymeric polypeptides and peptide-polymer conjugates

Nagy¹,

Májer

Hudecz

2000

Biopolymers

View full text Add to dashboard Cite

“…Fluorescence spectra were corrected for: (a) light scattering due to liposomes, (b) dilution effect, and (c) inner filter effect. Details are given in [9]. …”

Section: Vesicle Preparationmentioning

confidence: 95%

Physicochemical characterisation of four peptide sequences related to thrombospondin-1B

Reig

Ortiz²,

Alsina³

2011

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

“…Fluorescence studies were carried out with liposomes of different compositions prepared by the film method 16. Briefly, chloroform solutions of lipids were mixed in different volumes in order to have pure DPPC, DPPC/5%PG, or DPPC/20%PG.…”

Section: Methodsmentioning

confidence: 99%

Physicochemical characterization of branched chain polymeric polypeptide carriers based on a poly‐lysine backbone

Nagy¹,

Hudecz²,

Alsina³

et al. 2003

Biopolymers

View full text Add to dashboard Cite

A systematic study is reported on the physicochemical characteristics of two branched chain polymers (based on a poly-L-lysine backbone) with a general formula poly[Lys-(DL-Alam-Xi)], where X = Orn (OAK) or N-acetyl-Glu (Ac-EAK) and m approximately equal to 3, using surface pressure and fluorescence polarization methods. These data are compared with those of the linear poly(L-Lys) from which OAK and Ac-EAK are derived. These two polymers show a moderate surface activity, able to form stable monomolecular layers at the air-water interface. Poly(L-Lys), the most hydrophilic, has the lowest surface activity. The interaction of these polymers with phospholipid bilayers either neutral or negatively charged was studied with vesicles labeled with two fluorescent probes: ANS and DPH. Results indicate that these polymers are able to accommodate in their internal structure, mainly through electrostatic interactions, a certain amount of ANS marker molecules, but fluorescence increases of the ANS-polypeptide complexes were so low that its influence in further polarization measurements could be discarded. After interaction with liposomes, these polymers induce an increase in the polarization of the probes, thus indicating a rigidification of the bilayers. Electrostatic forces seem to be very important in this interaction; cationic polymers are clearly more active, with PG-containing liposomes, than Ac-EAK. Moreover, in these assays poly(L-Lys) behaves as the more active compound. This fact is probably due to its major ability to form alpha-helical structures that could insert easily in the bilayers. These results indicate that the polymeric structures studied can be used as carriers for biologically active molecules, because their interactions with bilayers remain soft and have a positive effect on the stability of the membranes.

show abstract

A fluorescence and CD study on the interaction of synthetic lipophilic hepatitis B virus preS(120-145) peptide analogues with phospholipid vesicles

Cited by 30 publications

References 37 publications

Effect of phospholipid bilayers on solution conformation of branched polymeric polypeptides and peptide-polymer conjugates

Effect of phospholipid bilayers on solution conformation of branched polymeric polypeptides and peptide-polymer conjugates

Physicochemical characterisation of four peptide sequences related to thrombospondin-1B

Physicochemical characterization of branched chain polymeric polypeptide carriers based on a poly‐lysine backbone

Contact Info

Product

Resources

About