A fluorescein-based fluorescence probe for the fast detection of thiol

A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application

“…Furthermore, the emission intensity at 450 nm was linearly related to the concentration of Cys from 0 to 50 μM (R 2 = 0.9925) ( Figure 6 ), which provided useful conditions for the quantitative analysis of Cys. And the detection limit of Cys was calculated to be 47.7 nM based on the 3σ/slope method [ 45 ]. The results indicated that the probe remained very sensitive to Cys.…”

Fluorescent “AND” logic gates for simultaneous detection of thiols and protons: photophysical properties, mechanism and bioimaging of living cells

“…[12][13][14][15][16][17] Abnormal levels of biological thiols could cause some diseases such as cardiovascular and neurodegenerative diseases, liver damage and cancer. [18][19][20][21][22] For several decades, the uorescent probe technique has been widely used in many scientic areas [23][24][25] taking advantage of ease of operation, high sensitivity, real-time detection and in vivo imaging. [26][27][28] Fluorescent probes for imaging reactive molecules or events in living organisms could be divided into two categories: organic small molecules and nanomaterials.…”

“…12–17 Abnormal levels of biological thiols could cause some diseases such as cardiovascular and neurodegenerative diseases, liver damage and cancer. 18–22…”

A 7-Hydroxybenzoxazinone-Containing Fluorescence Turn-On Probe for Biothiols and Its Bioimaging Applications

“…Great efforts have been directed to the development of fluorescent probes toward biothiols (Cys, Hcy, and GSH) [17,18,19,20,21,22,23]. Many probes have been constructed based on traditional fluorescent dyes, such as fluorescein [24], naphthalimide [25], coumarin [26], cyanin [27], BODIPY (boron-dipyrromethene) [23], xanthene [28], 2-(2′-hydroxyphenyl)benzothiazole [29], and dicyanomethylene-4 H -pyran derivatives [30]. In spite of some sensors with good sensing performance, many of these probes still suffer from poor solubility, low sensitivity, low quantum yield, and a time-consuming detection process.…”

“…We envisioned that masking the hydroxyl group in PBOH with a 2,4-dinitrophenylsulfonyl group (DNBS) could generate a new fluorescent probe ( PBD ) for the selective detection of biothiols (Scheme 1). As they are well-known, arenesulfonates and arenesulphonamides can be readily cleaved by thiolate anions, and, therefore, the 2,4-dintrobenzenesulfonyl (DNBS) group has often been chosen as the trigger group for thiols [24,34,35,36,37,38]. More importantly, the DNBS group can totally quench fluorescence, thus reducing background interference, because of its strong electron-withdrawing ability.…”