A flow-through nanoporous alumina trypsin bioreactor for mass spectrometry peptide fingerprinting

Kjellander, Marcus; Billinger, Erika; Ramachandraiah, Harisha; Boman, Mats; Lind, Sara Bergström; Johansson, Gunnar

doi:10.1016/j.jprot.2017.09.008

Cited by 4 publications

(4 citation statements)

References 23 publications

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Section: Other Supportsmentioning

confidence: 99%

“…The use of porous membranes fixed in membrane holders is also a popular choice [77,79]. Trypsin was immobilized on commercially available nanoporous anodized alumina membranes, creating a flow-through IMER that can be on-line coupled to ESI-TOF-MS [79]. The developed IMER was characterized by a high trypsin load due to the nanopores (pore size: 200 nm), as well as enhanced stability and straightforward assembly.…”

Section: Other Supportsmentioning

confidence: 99%

“…A method for ricin detection was developed using castor seeds [66]. Clinical samples like plasma [79], serum [16], tear [48,74], saliva [73] or dried blood spot samples [65] are popular due to their potential medical significance (e.g., biomarker discovery). Different types of cancer cells, namely hepatocarcinoma [67,68], breast cancer (MCF-7) [50], and HeLa cells [47,76] were analyzed.…”

Section: Proteomic Applicationsmentioning

confidence: 99%

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Microfluidic Immobilized Enzymatic Reactors for Proteomic Analyses—Recent Developments and Trends (2017–2021)

2022

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Given the strong interdisciplinary nature of microfluidic immobilized enzyme reactor (μ-IMER) technology, several branches of science contribute to its successful implementation. A combination of physical, chemical knowledge and engineering skills is often required. The development and application of μ-IMERs in the proteomic community are experiencing increasing importance due to their attractive features of enzyme reusability, shorter digestion times, the ability to handle minute volumes of sample and the prospect of on-line integration into analytical workflows. The aim of this review is to give an account of the current (2017–2021) trends regarding the preparation of microdevices, immobilization strategies, and IMER configurations. The different aspects of microfabrication (designs, fabrication technologies and detectors) and enzyme immobilization (empty and packed channels, and monolithic supports) are surveyed focusing on μ-IMERs developed for proteomic analysis. Based on the advantages and limitations of the published approaches and the different applications, a probable perspective is given.

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Section: Other Supportsmentioning

confidence: 99%

Section: Other Supportsmentioning

confidence: 99%

Section: Proteomic Applicationsmentioning

confidence: 99%

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Microfluidic Immobilized Enzymatic Reactors for Proteomic Analyses—Recent Developments and Trends (2017–2021)

2022

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“…The top-most advantage of using NAA templates is that the pore-properties of NAA such as pore diameter, pore-to-pore distance, porosity, pore-density (number of pores per unit area), and pore lengths and so on can be controlled in an easier way by adjusting the process parameters such as current, voltage, temperature, pH and so on. 2 The potential of NAA membranes have been successfully demonstrated in the synthesis of one-dimensional nanomaterials, [3][4][5] metallic nanowires, 6,7 photonic crystals, 8 and for optical biosensing [9][10][11][12] etc.…”

mentioning

confidence: 99%

Influence of Electrolyte Composition on the Photoluminescence and Pore Arrangement of Nanoporous Anodic Alumina

Choudhari

Kulkarni

Chidangil

et al. 2018

ECS J. Solid State Sci. Technol.

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Influence of oxalic and sulfuric acid electrolyte-mixture on the morphological and photoluminescence (PL) properties of Nanoporous Anodic Alumina (NAA) is reported. Two-dimensional fast Fourier transform of the FESEM images was employed to investigate the pore-arrangement of mixed electrolyte NAA. Pore-properties increased with sulfuric ion concentration and pore-regularity was highest with 1.4M sulfuric acid. Pore regularity is a direct consequence of sulfuric acid concentration at a fixed oxalic acid concentration (0.3M). No obvious PL was observed for NAA prepared in sulfuric acid while those prepared using mixed electrolytes showed strong blue emission. This broad PL comprised of four Gaussian curves (273, 316, 360 and 460nm). First two bands could be ascribed to luminescent centers transformed from sulfuric and oxalic impurities, respectively while the latter two are attributable to oxygen vacancies (F + and F-centers). PL bands blue-shifted with increasing intensities at higher sulfuric acid concentration. Emission intensity and wavelength could be tailored by varying the electrolyte composition.

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Immobilized enzyme-based analytical tools in the -omics era: Recent advances

Naldi

Tramarin

Bartolini

2018

Journal of Pharmaceutical and Biomedical Analysis

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A flow-through nanoporous alumina trypsin bioreactor for mass spectrometry peptide fingerprinting

Cited by 4 publications

References 23 publications

Microfluidic Immobilized Enzymatic Reactors for Proteomic Analyses—Recent Developments and Trends (2017–2021)

Microfluidic Immobilized Enzymatic Reactors for Proteomic Analyses—Recent Developments and Trends (2017–2021)

Influence of Electrolyte Composition on the Photoluminescence and Pore Arrangement of Nanoporous Anodic Alumina

Immobilized enzyme-based analytical tools in the -omics era: Recent advances

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