A flow–batch luminometer

Andrade, Renato Allan Navarro; Andrade, Stéfani Iury E.; Martins, Valdomiro Lacerda; Moreira, Pablo N.T.; Costa, Danielly Albuquerque da; Lyra, Wellington da Silva; Araújo, Mário César Ugulino de

doi:10.1016/j.microc.2012.10.009

Cited by 11 publications

(8 citation statements)

References 29 publications

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“…This difference is based on the cost of the equipment and the employed reagents in each case. It worth mentioning that the detection system for the proposed method can be constructed with low-cost electronic components like the luminometers shown in published works . In further works, the chemiluminescence detection needed for this method will be tested with low-cost electronics and smartphone cameras.…”

Section: Results and Discussionmentioning

confidence: 99%

“…It worth mentioning that the detection system for the proposed method can be constructed with low-cost electronic components like the luminometers shown in published works. 31 In further works, the chemiluminescence detection needed for this method will be tested with low-cost electronics and smartphone cameras. On the other hand, several methods have been developed to quantify FGL in biodiesel samples, as can be seen in Table 4.…”

Section: Variables Screening Study By Plackett-burman Designmentioning

confidence: 99%

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Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

et al. 2019

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The transesterification reaction to produce biodiesel generates a residual content of free glycerol, which may cause several problems to the engines. According to the international specification, the free glycerol determination involves a derivatization step followed by chromatographic analysis, which is done by advanced laboratories. Argentina is one of the largest biodiesel producers in the world, and part of this production is carried out by small and medium-sized producers. For those producers, this analysis is expensive and requires a considerable amount of time because there are few laboratories and they are localized far from the production site. This work proposes an alternative method for free glycerol determination in biodiesel samples taking advantage of automation, the use of an economic and available kit of enzymes, and a rapid and simple detection which was improved by the use of a fit-for-purpose chemiluminescence detection chamber designed in our laboratory. Quantification of free glycerol was performed by chemiluminescence at 425 nm after the reaction of hydrogen peroxide and luminol in the presence of Co2+. The hydrogen peroxide was generated by sequential enzymatic reactions based on the catalytic effect of glycerol kinase and glycerol phosphate oxidase. The calibration curve was linear in the 0.0045–0.0200% (w/w) range (r = 0.9910), and the sample throughput of the automatic system was 8 h–1. The geometrical configuration of the designed chemiluminescence detection chamber allowed an appropriate mixture of the reagents, due to a coupled efficient mixing system, and a highly sensitive detection (LOD value: 0.0013% (w/w)). The enzymatic method was applied for the determination of glycerol in commercial (soybean) and laboratory syntethized biodiesel samples (corn and sunflower). The trueness of the method was evaluated by comparison with the reference method (EN 14105), and a nonsignificative difference between them was observed (n = 6; P = 0.05).

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Section: Results and Discussionmentioning

confidence: 99%

Section: Variables Screening Study By Plackett-burman Designmentioning

confidence: 99%

Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

et al. 2019

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“…CL flow cells are made of silica, glass or transparent/translucent polymeric materials, and they are commercially available with limited designs. Many researchers have therefore designed and fabricated their own flow cells [ 2 , 11 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. Some flow cells have one flow path for the detection of CL light, with one inlet and one outlet [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the maximal level of light is not detected. Other flow cell designs have inlet ports located inside the detection chamber for the introduction of both samples and reagents [ 21 , 22 , 23 , 24 , 25 , 26 ]. There are mixing designs for rapid mixing, such as (i) magnetic stirring [ 21 ], (ii) vortex mixing [ 22 ] and (iii) swirling mixing [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Transparent Cross-Flow Platform as Chemiluminescence Detection Cell in Cross Injection Analysis

et al. 2023

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This work presents the use of a transparent ‘Cross Injection Analysis’ (CIA) platform as a flow system for chemiluminescence (CL) measurements. The CL-CIA flow device incorporates introduction channels for samples and reagents, and the reaction and detection channels are in one acrylic unit. A photomultiplier tube placed above the reaction channel detects the emitted luminescence. The system was applied to the analysis of (i) Co(II) via the Co(II)-catalyzed H2O2-luminol reaction and (ii) paracetamol via its inhibitory effect on the catalytic activity of Fe(CN)63− on the H2O2-luminol reaction. A linear calibration was obtained for Co(II) in the range of 0.002 to 0.025 mg L−1 Co(II) (r2 = 0.9977) for the determination of Co(II) in water samples. The linear calibration obtained for the paracetamol was 10 to 200 mg L−1 (r2 = 0.9906) for the determination of pharmaceutical products. The sample throughput was 60 samples h−1. The precision was ≤4.2% RSD. The consumption of the samples and reagents was ca. 170 µL per analysis cycle.

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“…As técnicas de detecção acopladas (hifenadas) aos sistemas de fluxobatelada são as mais variadas possíveis, entre elas destaca-se a espectrofotometria UV-vis [41,42], a quimiluminescência [43,44], espectrofluorimetria [45,46] e imagens digitais [47]. Este último tem ganhado bastante atenção nos últimos anos, por apresentar baixo custo e versatilidade instrumental [48].…”

Section: Sistemas Com Multicomutação Em Análises Em Fluxo (Mcfa)unclassified

Estratégias em fluxo para a determinação de acidez, sulfato e cloreto em etanol hidratado combustível

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A flow–batch luminometer

Cited by 11 publications

References 29 publications

Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

Transparent Cross-Flow Platform as Chemiluminescence Detection Cell in Cross Injection Analysis

Estratégias em fluxo para a determinação de acidez, sulfato e cloreto em etanol hidratado combustível

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