A flounder (Paralichthys olivaceus) gill cell line as in vitro acute assay system of nonylphenol cytotoxicity

Xiao, Qin; Li, Daizong; Liu, Hongying

doi:10.1007/s10661-010-1533-1

Cited by 11 publications

(3 citation statements)

References 22 publications

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“…In addition, the cytotoxicity of carbaryl on FG cells was closely correlated in all these assays, and was independent of the different cytotoxic endpoints. The results support the views of Ekwall (1995), Li and Zhang (2001) and Xiao et al (2011) that most of the cell lines have a similar response to toxicants when toxicity is measured with various endpoints relating to basal functions and structures. The results also suggest that the endpoints employed in the present study are useful to predict acute cytotoxicity.…”

Section: Discussionsupporting

confidence: 88%

“…The continuous marine flatfish cell line FG derived from flounder gill was maintained in our laboratory since its establishment by Tong et al (1997). It has already been successfully used to studying the toxic effects and mechanisms of environmental pollutants on fish species (Guo and Zhang, 2002;Li and Zhang, 2001;Na et al, 2009;Xiao et al, 2011;Xiao et al, 2007;Yang et al, 2010;Yin et al, 2007). However, the toxic effects of carbaryl on FG cells have not yet been evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos

Pandey

Guo

2015

J. Ocean Univ. China

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In this study, we determined the cytotoxicity and genotoxicity of carbamate insecticide carbaryl to flounder gill (FG) cells and its teratogenicity to zebrafish embryos. The cytotoxicity of carbaryl to FG cells was determined with methods including MTT and neutral red uptaking (NRU), lactate dehydrogenase (LDH) releasing and Hoechst 33342 and propidium idodide (PI) double staining. Moderate cytotoxicity in a concentration-dependent manner was observed. The 24 h-IC 50 value of 53.48 ± 1.21, 59.13 ± 1.19 and 46.21 ± 1.24 mg L −1 carbaryl was obtained through MTT, NRU and LDH assays, respectively. Double fluorescence staining demonstrated that carbaryl induced the death of FG cells mainly through necrosis. There was no significant genotoxicity found in the FG cells exposed to the highest testing concentration of carbaryl (20 mg L −1 , P > 0.05) as was demonstrated by Comet assay. Zebrafish embryos exposed to carbaryl at concentrations ≥10 mg L −1 displayed moderate toxic effects on the survival, spontaneous movement, hatching, heart rates of the embryos and their development, which were evidenced by yolk and pericardial sac edemas, body length reduction and tail flexure in time-and concentration-dependent manners at specific stages. The 24 h-, 48 h-and 96 h-LC 50 values of carbaryl to zebrafish embryos were 41.80 ± 1.10, 17.80 ± 1.04 and 14.46 ± 1.05 mg L −1 , respectively. These results suggested that carbaryl is moderately toxic to FG cells cultured in vitro and zebrafish embryos, and the FG cells were similar to zebrafish embryos in their sensitivity to carbaryl as 24 h-IC 50 and LC 50 indicated.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos

Pandey

Guo

2015

J. Ocean Univ. China

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“…Over the past few decades, artificial breeding (Rothbard et al ., ), aquaculture (Li et al ., ), immunity (Zhang et al ., ; Zhou et al ., ) and the biocontrol of molluscs and trematode on black carp have been investigated successively (Hung et al ., ), while cell lines derived from this species are rarely available except for the kidney (Xue et al ., ). As of today, the black carp aquaculture industry faces difficulties due to the infection of many pathogenic microorganisms including the spring viremia of carp virus (SVCV; Xiao et al ., ), which require a cell line susceptible to the virus as a convenient in vitro platform for pathogen‐host interaction studies in black carp.…”

Section: Introductionmentioning

confidence: 99%

Virus susceptibility of a new cell line derived from the fin of black carp Mylopharyngodon piceus

Wang

Xue

Wang

et al. 2019

Journal of Fish Biology

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A continuous cell line MPF derived from the fin of black carp Mylopharyngodon piceus was established and characterised in this study. Mylopharyngodon piceus fin (MPF) cells were subcultured for more than 80 passages with high viability recovery after long-term storage. The karyotyping analysis revealed that MPF had a modal diploid chromosome number (2n = 48) and identical ribosomal RNA sequence with black carp. In addition, the expression of pluripotency-associated markers including nanog, oct4 and vasa, were detected in MPF. The transient transfection efficiency of MPF reached 23% with a fluorescent reporter by modified electroporation and stable expression of red fluorescent MPF was established by the baculovirus system, indicating that MPF is an ideal platform for studying gene functions in vitro. Lastly, cytopathic effects were also observed and RNA transcripts of a viral gene increased after infection by spring viremia of carp virus (SVCV), suggesting that MPF could be an alternative tool for investigating pathogen-host interactions in black carp. In conclusion, a fin cell line that is susceptible to SVCV was established as a potential adult stem-cell line, providing a suitable tool for future genetic analyses and pathogenhost studies in black carp. K E Y W O R D S black carp, cell line, fin, Mylopharyngodon piceus, pluripotency, SVCV

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Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala

Zhu

Yang

Wang

et al. 2013

Fish Physiol Biochem

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This study established and characterized a new cell line (MAF) from the fin of blunt snout bream (Megalobrama amblycephala), a freshwater fish cultivated in China. MAF cells proliferated well in medium 199 supplemented with 10 % fetal bovine serum at 28 °C and have been subcultured more than 95 times in almost a year. MAF cells were revived at 90-95 % viability after 3-6 months of storage in liquid nitrogen. Karyotyping indicated that the modal chromosome number of MAF cells was 48. The MAF cell line consisted predominantly of fibroblastic and epithelial-like cells from M. amblycephala, which was confirmed by immunofluorescence and mitochondrial 12s rRNA sequencing. Viral susceptibility tests showed that MAF cells were susceptible to infection by snakehead rhabdovirus, spring viremia carp virus, and channel catfish virus, which was demonstrated by the presence of cytopathic effect, high viral titers, and PCR products. Bacterial cytotoxicity studies showed that extracellular products from Aeromonas hydrophila were toxic to MAF cells. Cu²⁺ was also cytotoxic to MAF cells, and the 24-h IC₅₀ value was 144.48 μmol/l. When MAF cells were transfected with pEGFP-N1 plasmid, bright fluorescent signals were observed, and the transfection efficiency reached up to 5 %. These results suggest that the MAF cell line may provide a valuable tool for studying virus pathogenesis, as well as cytotoxicity testing and genetic manipulation studies.

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A flounder (Paralichthys olivaceus) gill cell line as in vitro acute assay system of nonylphenol cytotoxicity

Cited by 11 publications

References 22 publications

Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos

Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos

Virus susceptibility of a new cell line derived from the fin of black carp Mylopharyngodon piceus

Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala

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