A Floating Mold Technique for the Programmed Assembly of Protocells into Protocellular Materials Capable of Non‐Equilibrium Biochemical Sensing

Galanti, Agostino; Moreno-Tortolero, Rafael O; Azad, Raihan; Cross, Stephen; Davis, Sean A.; Gobbo, Pierangelo

doi:10.1002/adma.202100340

Cited by 25 publications

(28 citation statements)

References 24 publications

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“…Other than protein pore-mediated direct signaling, there are few examples where proteinosomes have been cross-linked using covalent bonds to form synthetic tissues exhibiting internal signaling ( Figure 2B ). Gobbo et al used covalent biorthogonal chemistry to crosslink proteinosomes to construct artificial prototissues ( Gobbo et al, 2018 ) and recently showed direct signal exchange between covalently crosslinked GOx and HRP-loaded artificial cells to produce resorufin as final product ( Galanti et al, 2021 ). In this case, the high molecular cut-off of the proteinosome membrane allows fast internal signaling within the synthetic tissues.…”

Section: Distance-based Classification Of Artificial Cell Communicationmentioning

confidence: 99%

Chemical Communication in Artificial Cells: Basic Concepts, Design and Challenges

Karoui

Patwal

Kumar

et al. 2022

Front. Mol. Biosci.

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In the past decade, the focus of bottom-up synthetic biology has shifted from the design of complex artificial cell architectures to the design of interactions between artificial cells mediated by physical and chemical cues. Engineering communication between artificial cells is crucial for the realization of coordinated dynamic behaviours in artificial cell populations, which would have implications for biotechnology, advanced colloidal materials and regenerative medicine. In this review, we focus our discussion on molecular communication between artificial cells. We cover basic concepts such as the importance of compartmentalization, the metabolic machinery driving signaling across cell boundaries and the different modes of communication used. The various studies in artificial cell signaling have been classified based on the distance between sender and receiver cells, just like in biology into autocrine, juxtacrine, paracrine and endocrine signaling. Emerging tools available for the design of dynamic and adaptive signaling are highlighted and some recent advances of signaling-enabled collective behaviours, such as quorum sensing, travelling pulses and predator-prey behaviour, are also discussed.

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Section: Distance-based Classification Of Artificial Cell Communicationmentioning

confidence: 99%

Chemical Communication in Artificial Cells: Basic Concepts, Design and Challenges

Karoui

Patwal

Kumar

et al. 2022

Front. Mol. Biosci.

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“…In addition to micelles, vesicles can be incorporated into hydrogels as well (Figure 2d,e), e.g., liposomes based on lipids, [100,101] niosomes based on nonionic surfactants, [102,103] vesicles from cellular origin, [104] vesicles based on ionic surfactants, [105][106][107] proteinosomes based on protein-polymer conjugates [108,109] or polymersomes based on block copolymers. [110] A feature of vesicular structures is the membrane that separates the interior aqueous medium from the exterior aqueous medium, which contains a hydrophobic barrier.…”

Section: Vesiclesmentioning

confidence: 99%

Multicompartment Hydrogels

Schmidt

2022

Macromol. Rapid Commun.

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Hydrogels belong to the most promising materials in polymer and materials science at the moment. As they feature soft and tissue-like character as well as high water-content, a broad range of applications are addressed with hydrogels, e.g., tissue engineering and wound dressings but also soft robotics, drug delivery, actuators, and catalysis. Ways to tailor hydrogel properties are crosslinking mechanisms, hydrogel shape, and reinforcement, but new features can be introduced by variation of hydrogel composition as well, e.g., via monomer choice, functionalization or compartmentalization. In particular, multicompartment hydrogels drive progress toward complex and highly functional soft materials. In the present review the latest developments in multicompartment hydrogels are highlighted with a focus on three types of compartments; micellar/vesicular, droplets, and multilayers including various subcategories. Furthermore, several morphologies of compartmentalized hydrogels and applications of multicompartment hydrogels will be discussed as well. Finally, an outlook toward future developments of the field will be given. The further development of multicompartment hydrogels is highly relevant for a broad range of applications and will have a significant impact on biomedicine and organic devices.

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“…(E) Schematic of chemical communication based on a GOx/HRP enzyme cascade reaction within a protocellular material. Details on the reactivity are reported in (d) (Galanti et al, 2021). (F) Mechanism of the chemical "translation" carried out by protocells for murine stem cells.…”

Section: Emergence Of Advanced Biomimetic Behavioursmentioning

confidence: 99%

“…PCMs are centimetre sized assemblies of covalently ligated azide-and strained alkyne-functionalised protocells that are robust, free standing, stable in water, and capable of chemical communication (Figure 2E). (Galanti et al, 2021) Most importantly, PCMs can be easily manipulated by hand making them ideal candidates for a variety of different applications from materials science to regenerative medicine.…”

Section: Interconnected Protocell Networkmentioning

confidence: 99%

Bioinspired Networks of Communicating Synthetic Protocells

Grimes

Galanti

Gobbo

2021

Front. Mol. Biosci.

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The bottom-up synthesis of cell-like entities or protocells from inanimate molecules and materials is one of the grand challenges of our time. In the past decade, researchers in the emerging field of bottom-up synthetic biology have developed different protocell models and engineered them to mimic one or more abilities of biological cells, such as information transcription and translation, adhesion, and enzyme-mediated metabolism. Whilst thus far efforts have focused on increasing the biochemical complexity of individual protocells, an emerging challenge in bottom-up synthetic biology is the development of networks of communicating synthetic protocells. The possibility of engineering multi-protocellular systems capable of sending and receiving chemical signals to trigger individual or collective programmed cell-like behaviours or for communicating with living cells and tissues would lead to major scientific breakthroughs with important applications in biotechnology, tissue engineering and regenerative medicine. This mini-review will discuss this new, emerging area of bottom-up synthetic biology and will introduce three types of bioinspired networks of communicating synthetic protocells that have recently emerged.

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A Floating Mold Technique for the Programmed Assembly of Protocells into Protocellular Materials Capable of Non‐Equilibrium Biochemical Sensing

Cited by 25 publications

References 24 publications

Chemical Communication in Artificial Cells: Basic Concepts, Design and Challenges

Chemical Communication in Artificial Cells: Basic Concepts, Design and Challenges

Multicompartment Hydrogels

Bioinspired Networks of Communicating Synthetic Protocells

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