A flexible, efficient, checkerboard immunoblot system for the detection and semiquantitation of specific antinuclear antibodies

Ritchie, Robert F.; Collins, Marilyn F.; Ledue, Thomas B.

doi:10.1002/jcla.1860060610

Cited by 9 publications

(3 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was subsequently used for qualitative and semiquantitative clinical applications in human sera. Ritchie et al (9) have applied the method to specific antinuclear antibodies determination and Craig et al (10) to antiviral IgG measurements (10). As a complement to these interesting possibilities, we present a rather original application of CBIB to assess specific IgE in allergic patients sera.…”

Section: Introduction: Testing For Allergen-specific Ige Antibodymentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous quantitation of specific IgE against 20 purified allergens in allergic patients sera by checkerboard immunoblotting (CBIB)

Musu

Rabillon

Pelletier

et al. 1997

J. Clin. Lab. Anal.

View full text Add to dashboard Cite

A simple technique, checkerboard immunoblotting (CBIB), is described for simultaneous quantitation of specific IgE antibodies against several allergens in human sera. Using as little as 50 μl of each of the 20 sera examined against 20 different allergens, it was possible in a single run to achieve 400 tests. To guarantee high specificity and sensitivity of the assay, this new application of CBIB employs purified allergens, cyanogen bromide‐activated nitrocellulose membrane, and Phosphorimager technology. Results are expressed both qualitatively (five classes) and quantitatively in kilo units per liter equilibrated against the World Health Organization (WHO) standard for IgE. There was excellent agreement between the results of CBIB and the results of Pharmacia Cap System, an alternative method widely used for measuring serum‐specific IgE. The CBIB method certainly could be useful in any laboratory interested in allergy clinical research for easy screening and relative quantitation of allergen‐specific human IgE. J. Clin. Lab. Anal. 11:357–362, 1997.© 1997 Wiley‐Liss, Inc.

show abstract

Section: Introduction: Testing For Allergen-specific Ige Antibodymentioning

confidence: 99%

“…The effect of coating a CNBr-activated nitro cellulose membrane with increasing concentrations of Der p1 (Z), Dac g1(9) and Fel d1(7). The respective specific IgE were detected with 125 I radioactive labelled monoclonal antibodies antihuman IgE and quantified by Phosphorimager analysis.…”

mentioning

confidence: 99%

Simultaneous quantitation of specific IgE against 20 purified allergens in allergic patients sera by checkerboard immunoblotting (CBIB)

Musu

Rabillon

Pelletier

et al. 1997

J. Clin. Lab. Anal.

View full text Add to dashboard Cite

show abstract

“…The effect of coating a CNBr-activated nitro cellulose membrane with increasing concentrations of Der p1 (Z), Dac g1(9) and Fel d1(7).The respective specific IgE were detected with 125 I radioactive labelled monoclonal antibodies antihuman IgE and quantified by Phosphorimager analysis. Linear correlation coefficient (r 2 ): Der p1, r 2 = 0.994; Dac g1, r 2 = 0.996 and Fel d1, r 2 = 0.991.…”

mentioning

confidence: 99%

Simultaneous quantitation of specific IgE against 20 purified allergens in allergic patients sera by checkerboard immunoblotting (CBIB)

Musu

Rabillon

Pelletier

et al. 1997

J. Clin. Lab. Anal.

View full text Add to dashboard Cite

A simple technique, checkerboard immunoblotting (CBIB), is described for simultaneous quantitation of specific IgE antibodies against several allergens in human sera. Using as little as 50 microliters of each of the 20 sera examined against 20 different allergens, it was possible in a single run to achieve 400 tests. To guarantee high specificity and sensitivity of the assay, this new application of CBIB employs purified allergens, cyanogen bromide-activated nitrocellulose membrane, and Phosphorimager technology. Results are expressed both qualitatively (five classes) and quantitatively in kilo units per liter equilibrated against the World Health Organization (WHO) standard for IgE. There was excellent agreement between the results of CBIB and the results of Pharmacia Cap System, an alternative method widely used for measuring serum-specific IgE. The CBIB method certainly could be useful in any laboratory interested in allergy clinical research for easy screening and relative quantitation of allergen-specific human IgE.

show abstract

Serum Proteins

Ritchie

Whicher

2000

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Plasma contains a large number of different proteins with varying structural characteristics. The “plasma proteins” have been defined on the basis of plasma concentration and amenability to measurement in certain types of measurement systems. In the past, they were proteins present with concentrations above 0.05 g L −1 , but more recently detection limits have been decreased to about 0.001 g L −1 . However, the group of proteins is not defined on a pathophysiological basis and the definition remains idiosyncratic and incomprehensible to the noncognoscenti. The function of an increasing proportion of them is known and the evidence suggests that they are present either with a specific role in the plasma or that they represent cellular proteins shed into the circulation as a result of degradative processes. Many plasma proteins show characteristic changes in concentration or structure either resulting from genetic dictates that may give rise to disease directly or as a result of changes reflecting pathological or physiological processes. All the transcellular fluids, such as cerebrospinal fluid (CSF), urine, synovial fluid and saliva, contain most or all of the plasma proteins, but there are some additional proteins secreted specifically into these fluids. This chapter reviews the many methods applied to proteins for clinical and research purposes. Also addressed are the pitfalls not often appreciated by the worker presented with a sample to be analyzed in an increasingly impenetrable “machine”.

show abstract

A flexible, efficient, checkerboard immunoblot system for the detection and semiquantitation of specific antinuclear antibodies

Cited by 9 publications

References 48 publications

Simultaneous quantitation of specific IgE against 20 purified allergens in allergic patients sera by checkerboard immunoblotting (CBIB)

Simultaneous quantitation of specific IgE against 20 purified allergens in allergic patients sera by checkerboard immunoblotting (CBIB)

Simultaneous quantitation of specific IgE against 20 purified allergens in allergic patients sera by checkerboard immunoblotting (CBIB)

Serum Proteins

Contact Info

Product

Resources

About