A flagellin-producing Lactococcus strain: interactions with mucin and enteropathogens

Sánchez, Borja; López, Patricia; González-Rodríguez, Irene; Suárez, Ana; Margollés, Abelardo; Urdaci, María C.

doi:10.1111/j.1574-6968.2011.02244.x

Cited by 24 publications

(15 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial flagella are complex organelles that improve cell motility, enable chemotaxis, contribute to biofilm structure 42 , and aid in mucin adhesion for a number of species 23,40,[43][44][45][46] .…”

Section: Resultsmentioning

confidence: 99%

Role of Flagella in Adhesion of Escherichia coli to Abiotic Surfaces

Friedlander

Vogel²,

Aizenberg³

2015

Langmuir

100

105

View full text Add to dashboard Cite

The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters. Abstract: Understanding the interfacial activity of bacteria is of critical importance due to the huge economic and public health implications associated with surface fouling and biofilm formation. The complexity of the process and difficulties of predicting microbial adhesion to novel materials demand study of the properties of specific bacterial surface features and their potential contribution to surface attachment. Here, we examine flagella, cell appendages primarily studied for their cell motility function, to elucidate their role in surface adhesion of Escherichia coli-a model organism and potential pathogen. We use self-assembled monolayers (SAMs) of thiol-bearing molecules on gold films to generate surfaces of varying hydrophobicity, and measure adhesion of purified flagella using quartz crystal microbalance. We show that flagella adhere more extensively and bind more tightly to hydrophobic SAMs than to hydrophilic ones, and propose a two-step vs a single-step adhesion mechanism that accounts for the observed dissipation and frequency changes for the two types of surfaces, respectively. Subsequently, study of the adhesion of wild-type and flagella knock-out cells confirms that flagella improve adhesion to hydrophobic substrates, whereas cells lacking flagella do not show preferred affinity to hydrophobic substrates. Together, these properties bring about an interesting ability of cells with flagella to stabilize emulsions of aqueous culture and dodecane, not observed for cells lacking flagella. This work contributes to our overall understanding of nonspecific bacterial adhesion and suggests that flagella, beyond motility, may play an important role in surface adhesion.Introduction:

show abstract

Section: Resultsmentioning

confidence: 99%

Role of Flagella in Adhesion of Escherichia coli to Abiotic Surfaces

Friedlander

Vogel²,

Aizenberg³

2015

Langmuir

100

105

View full text Add to dashboard Cite

show abstract

“…Lactobacilli can exert health benefits in several ways, for example by inhibiting growth of pathogenic bacteria as they produce lactic, propionic, and acetic acids lowering the pH that suppresses proliferation of pathogens in the GIT [ 20 , 30 , 31 ]. Additionally, they can competitively prevent attachment of pathogens to the epithelium [ 16 , 32 , 33 , 34 , 35 ]. Lactobacilli have also been connected with improvements of gut diseases or disease-associated symptoms.…”

Section: Gastrointestinal Tract Mucins and Lactobacillimentioning

confidence: 99%

Dietary Nutrients, Proteomes, and Adhesion of Probiotic Lactobacilli to Mucin and Host Epithelial Cells

Çelebioğlu

Svensson

2018

Microorganisms

View full text Add to dashboard Cite

The key role of diet and environment in human health receives increasing attention. Thus functional foods, probiotics, prebiotics, and synbiotics with beneficial effects on health and ability to prevent diseases are in focus. The efficacy of probiotic bacteria has been connected with their adherence to the host epithelium and residence in the gut. Several in vitro techniques are available for analyzing bacterial interactions with mucin and intestinal cells, simulating adhesion to the host in vivo. Proteomics has monitored and identified proteins of probiotic bacteria showing differential abundance elicited in vitro by exposure to food components, including potential prebiotics (e.g., certain carbohydrates, and plant polyphenols). While adhesion of probiotic bacteria influenced by various environmental factors relevant to the gastrointestinal tract has been measured previously, this was rarely correlated with changes in the bacterial proteome induced by dietary nutrients. The present mini-review deals with effects of selected emerging prebiotics, food components and ingredients on the adhesion of probiotic lactobacilli to mucin and gut epithelial cells and concomitant abundancy changes of specific bacterial proteins. Applying this in vitro synbiotics-like approach enabled identification of moonlighting and other surface-located proteins of Lactobacillus acidophilus NCFM that are possibly associated with the adhesive mechanism.

show abstract

“…Paton et al have engineered E. coli strains producing chimeric surface lipopolysaccharides that are capable of neutralizing, on contact, shiga-toxin secreted by pathogenic E. coli or cholera toxin secreted by V. cholerae in vivo [22,23]. Probiotic lactic acid bacteria (LAB) have been engineered to secrete either pathogenic adhesion proteins or flagellin in order to competitively inhibit adhesion of pathogens such as Listeria monocytogenes or Salmonella enterica [24,25]. These studies aimed to engineer commensal bacteria to outcompete for valuable surface space in the host s intestine, forcing pathogens to pass through the gastrointestinal tract without detrimental effects.…”

Section: Engineering Strategies Targeting Bacterial Infectionmentioning

confidence: 99%

Engineering commensal bacteria for prophylaxis against infection

Goh

March

2012

Current Opinion in Biotechnology

View full text Add to dashboard Cite

Infectious diseases are the leading causes of death worldwide. The development of efficient and low cost prophylactics to prevent pathogenic infection is given high priority in the twenty-first century. Commensal bacteria are largely seen as harmless and can survive symbiotically (in many cases) in niches throughout the human body. Advances in genetic engineering and understanding of pathogenesis have revealed many potential strategies to develop engineered bacteria for prophylaxis purposes: including live vaccines and anti-infective agents. In this review we discuss recent advances and potentialities of prophylaxis with engineered bacteria.

show abstract

A flagellin-producing Lactococcus strain: interactions with mucin and enteropathogens

Cited by 24 publications

References 39 publications

Role of Flagella in Adhesion of Escherichia coli to Abiotic Surfaces

Role of Flagella in Adhesion of Escherichia coli to Abiotic Surfaces

Dietary Nutrients, Proteomes, and Adhesion of Probiotic Lactobacilli to Mucin and Host Epithelial Cells

Engineering commensal bacteria for prophylaxis against infection

Contact Info

Product

Resources

About