A First in Human Trial Implanting Microalgae Shows Safety of Photosynthetic Therapy for the Effective Treatment of Full Thickness Skin Wounds

Obaíd, Miguel; Camacho, Juan Pablo; Brenet, Marianne; Corrales-Orovio, Rocío; Carvajal, Felipe; Martorell, Ximena; Werner, C. Scudieri Paes; Simón, Valeska; Varas, Juan; Calderón, Wilfredo; Egaña, José Tomás; Bono, Marı́a Rosa; Martín, Sebastián San; Eblén-Zajjur, Antonio

doi:10.3389/fmed.2021.772324

Cited by 31 publications

(45 citation statements)

References 38 publications

(51 reference statements)

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“…Although the culture conditions, 30°C and a simple mix medium of mammalian cell culture and algal medium (50:50 mixture), were a compromise for both species, the expression of the hypoxia-inducible factor HIF-1α in the fibroblasts could be reduced by the presence of microalgae. Follow-up studies of this team demonstrated the biocompatibility, safety and potential applicability of microalgae-laden commercial collagen scaffolds for photosynthetic therapies in a mouse full skin defect as well as in a first clinical trial comprising eight patients with full thickness skin wounds ( Schenck et al, 2015 ; Chávez et al, 2016 ; Obaíd et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Selection of a suitable photosynthetically active microalgae strain for the co-cultivation with mammalian cells

Dani

Windisch

Guerrero

et al. 2022

Front. Bioeng. Biotechnol.

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Preventing hypoxic zones in 3D bioprinted mammalian cell-laden constructs using an internal oxygen supply could enable a more successful cultivation both in vitro and in vivo. In this study, the suitability of green microalgae as photosynthetic oxygen generators within bioprinted constructs was evaluated by defining and investigating important parameters for a successful co-culture. First, we assessed the impact of light–necessary for photosynthesis–on two non-light adapted mammalian cell types and defined red-light illumination and a temperature of 37°C as essential factors in a co-culture. The four thermotolerant microalgae strains Chlorella sorokiniana, Coelastrella oocystiformis, Coelastrella striolata, and Scenedesmus sp. were cultured both in suspension culture and 3D bioprinted constructs to assess viability and photosynthetic activity under these defined co-culture conditions. Scenedesmus sp. proved to be performing best under red light and 37°C as well as immobilized in a bioprinted hydrogel based on alginate. Moreover, the presence of the antibiotic ampicillin and the organic carbon-source glucose, both required for mammalian cell cultures, had no impact on bioprinted Scenedesmus sp. cultures regarding growth, viability, and photosynthetic activity. This study is the first to investigate the influence of mammalian cell requirements on the metabolism and photosynthetic ability of different microalgal strains. In a co-culture, the strain Scenedesmus sp. could provide a stable oxygenation that ensures the functionality of the mammalian cells.

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Section: Introductionmentioning

confidence: 99%

Selection of a suitable photosynthetically active microalgae strain for the co-cultivation with mammalian cells

Dani

Windisch

Guerrero

et al. 2022

Front. Bioeng. Biotechnol.

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“…This approach has been described by our group in recent in vitro ( Hopfner et al, 2014 ; Centeno-Cerdas et al, 2018 ; Chávez et al, 2021 ) and in vivo ( Schenck et al, 2015 ; Chávez et al, 2016 ) studies, and further confirmed by other independent groups, highlighting its potential application in several medical fields, including tissue engineering and regeneration ( Yamaoka et al, 2012 ; Evron et al, 2015 ; Haraguchi et al, 2017 ; Chen et al, 2020 ), heart ischemia ( Cohen et al, 2017 ), and tumor treatment ( Huo et al, 2020 ; Liu et al, 2020 ; Qiao et al, 2020 ). Moreover, an ongoing clinical trial is confirming its safety for tissue regeneration in humans ( Obaíd et al (2021) accepted for publication. ClinicalTrials.gov Identifier: NCT03960164).…”

Section: Introductionmentioning

confidence: 93%

Development of a Novel Perfusable Solution for ex vivo Preservation: Towards Photosynthetic Oxygenation for Organ Transplantation

Veloso-Giménez

Escamilla

Necuñir

et al. 2021

Front. Bioeng. Biotechnol.

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Oxygen is the key molecule for aerobic metabolism, but no animal cells can produce it, creating an extreme dependency on external supply. In contrast, microalgae are photosynthetic microorganisms, therefore, they are able to produce oxygen as plant cells do. As hypoxia is one of the main issues in organ transplantation, especially during preservation, the main goal of this work was to develop the first generation of perfusable photosynthetic solutions, exploring its feasibility for ex vivo organ preservation. Here, the microalgae Chlamydomonas reinhardtii was incorporated in a standard preservation solution, and key aspects such as alterations in cell size, oxygen production and survival were studied. Osmolarity and rheological features of the photosynthetic solution were comparable to human blood. In terms of functionality, the photosynthetic solution proved to be not harmful and to provide sufficient oxygen to support the metabolic requirement of zebrafish larvae and rat kidney slices. Thereafter, isolated porcine kidneys were perfused, and microalgae reached all renal vasculature, without inducing damage. After perfusion and flushing, no signs of tissue damage were detected, and recovered microalgae survived the process. Altogether, this work proposes the use of photosynthetic microorganisms as vascular oxygen factories to generate and deliver oxygen in isolated organs, representing a novel and promising strategy for organ preservation.

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“…Together these results suggest the clinical translation potential of photosynthetic biomaterials. 105…”

Section: Oxygenation Strategies For Wound Healingmentioning

confidence: 99%

Oxygenation therapies for improved wound healing: current trends and technologies

et al. 2022

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A First in Human Trial Implanting Microalgae Shows Safety of Photosynthetic Therapy for the Effective Treatment of Full Thickness Skin Wounds

Cited by 31 publications

References 38 publications

Selection of a suitable photosynthetically active microalgae strain for the co-cultivation with mammalian cells

Selection of a suitable photosynthetically active microalgae strain for the co-cultivation with mammalian cells

Development of a Novel Perfusable Solution for ex vivo Preservation: Towards Photosynthetic Oxygenation for Organ Transplantation

Oxygenation therapies for improved wound healing: current trends and technologies

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